2022
Predicting the locations of force-generating dyneins in beating cilia and flagella
Howard J, Chasteen A, Ouyang X, Geyer VF, Sartori P. Predicting the locations of force-generating dyneins in beating cilia and flagella. Frontiers In Cell And Developmental Biology 2022, 10: 995847. PMID: 36303602, PMCID: PMC9592896, DOI: 10.3389/fcell.2022.995847.Peer-Reviewed Original ResearchDynein motorsDynein motor proteinsCryogenic electron microscopyMotor proteinsCylindrical organellesDyneinSperm flagellaConformational statesFlagellaAxonemeMotile structuresCiliaThree-dimensional architectureSingle pairOrganellesATPasesIndividual motor activityProteinMolecular mechanics approachActivity changesLarge numberCellsKey questionsCounting fluorescently labeled proteins in tissues in the spinning–disk microscope using single–molecule calibrations
Liao M, Kuo Y, Howard J. Counting fluorescently labeled proteins in tissues in the spinning–disk microscope using single–molecule calibrations. Molecular Biology Of The Cell 2022, 33: ar48. PMID: 35323029, PMCID: PMC9265152, DOI: 10.1091/mbc.e21-12-0618.Peer-Reviewed Original ResearchConceptsEnd-binding protein 1Spinning-disk confocal microscopyConfocal microscopySingle-molecule imagingComplex biological phenomenaFly larvaeLiving cellsCell surfaceMicrotubule endsBiological phenomenaProtein 1Brightness of fluorophoresCytoplasmic concentrationEpifluorescence microscopeBiological systemsSensory neuronsCellsSingle moleculesAbsolute numberMolecular numberTissueLarvaeMicrotubulesEGFPProtein
2016
A force-generating machinery maintains the spindle at the cell center during mitosis
Garzon-Coral C, Fantana HA, Howard J. A force-generating machinery maintains the spindle at the cell center during mitosis. Science 2016, 352: 1124-1127. PMID: 27230381, PMCID: PMC6535051, DOI: 10.1126/science.aad9745.Peer-Reviewed Original ResearchConceptsForce-generating machineryMitotic spindleAsymmetric divisionDaughter cellsCell centerIntact cellsMagnetic tweezersCorrect localizationNumber of microtubulesMachinerySpindleForce generatorCellsCaenorhabditisAnaphaseLocalizationAccurate partitionsMitosisEmbryosMicrotubulesCytoplasmMetaphasePrecise localizationSmall cellsDivision
2006
Yeast kinesin-8 depolymerizes microtubules in a length-dependent manner
Varga V, Helenius J, Tanaka K, Hyman AA, Tanaka TU, Howard J. Yeast kinesin-8 depolymerizes microtubules in a length-dependent manner. Nature Cell Biology 2006, 8: 957-962. PMID: 16906145, DOI: 10.1038/ncb1462.Peer-Reviewed Original ResearchConceptsKinesin-8 proteinsLength-dependent depolymerizationYeast kinesin-8Length-dependent mannerMetazoan cellsMicrotubule cytoskeletonMitotic spindleDisassemble microtubulesSpindle lengthKinesin-8Kinesin-13Kip3pPlus endsMicrotubulesConstituent microtubulesLong microtubulesNew mechanismCytoskeletonFungiKinesinProteinSpindleCells
1988
Compliance of the hair bundle associated with gating of mechanoelectrical transduction channels in the Bullfrog's saccular hair cell
Howard J, Hudspeth AJ. Compliance of the hair bundle associated with gating of mechanoelectrical transduction channels in the Bullfrog's saccular hair cell. Neuron 1988, 1: 189-199. PMID: 2483095, DOI: 10.1016/0896-6273(88)90139-0.Peer-Reviewed Original ResearchConceptsTransduction channelsSaccular hair cellsHair bundlesHair cellsMechanoelectrical transduction channelsGating springMolecular basisBullfrog saccular hair cellsChannel gateGating complianceConformational changesMechanoelectrical transductionCellsMechanical stimuliTransductionSpringMechanical stimulationGating
1987
Mechanical relaxation of the hair bundle mediates adaptation in mechanoelectrical transduction by the bullfrog's saccular hair cell.
Howard J, Hudspeth AJ. Mechanical relaxation of the hair bundle mediates adaptation in mechanoelectrical transduction by the bullfrog's saccular hair cell. Proceedings Of The National Academy Of Sciences Of The United States Of America 1987, 84: 3064-3068. PMID: 3495007, PMCID: PMC304803, DOI: 10.1073/pnas.84.9.3064.Peer-Reviewed Original Research