1993
Mutations in the conserved loop of human U5 snRNA generate use of novel cryptic 5′ splice sites in vivo.
Cortes JJ, Sontheimer EJ, Seiwert SD, Steitz JA. Mutations in the conserved loop of human U5 snRNA generate use of novel cryptic 5′ splice sites in vivo. The EMBO Journal 1993, 12: 5181-5189. PMID: 8262061, PMCID: PMC413781, DOI: 10.1002/j.1460-2075.1993.tb06213.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceGlobinsHumansMolecular Sequence DataMutationNucleic Acid PrecursorsOligodeoxyribonucleotidesRabbitsRNA SplicingRNA, MessengerRNA, Small NuclearConceptsSplice siteRabbit beta-globin geneBeta-globin transcriptsBeta-globin geneU5 genesConserved loopMRNA splicingU5 snRNASecond intronFirst exonExon sequencesLoop mutantsSecond exonExpression vectorTransient transfectionCryptic sitesNovel siteHeLa cellsGT dinucleotideExonsExon 1U5 sequencesSnRNAMutationsVivo system
1987
Multiple interactions between the splicing substrate and small nuclear ribonucleoproteins in spliceosomes.
Chabot B, Steitz JA. Multiple interactions between the splicing substrate and small nuclear ribonucleoproteins in spliceosomes. Molecular And Cellular Biology 1987, 7: 281-293. PMID: 2951586, PMCID: PMC365068, DOI: 10.1128/mcb.7.1.281.Peer-Reviewed Original Research
1986
A protein associated with small nuclear ribonucleoprotein particles recognizes the 3′ splice site of premessenger RNA
Gerke V, Steitz J. A protein associated with small nuclear ribonucleoprotein particles recognizes the 3′ splice site of premessenger RNA. Cell 1986, 47: 973-984. PMID: 2946421, DOI: 10.1016/0092-8674(86)90812-3.Peer-Reviewed Original ResearchPre-mRNA splicing in vitro requires intact U4/U6 small nuclear ribonucleoprotein
Black D, Steitz J. Pre-mRNA splicing in vitro requires intact U4/U6 small nuclear ribonucleoprotein. Cell 1986, 46: 697-704. PMID: 2427202, DOI: 10.1016/0092-8674(86)90345-4.Peer-Reviewed Original ResearchMeSH KeywordsCell-Free SystemHeLa CellsHumansNucleic Acid PrecursorsRibonucleoproteinsRibonucleoproteins, Small NuclearRNARNA PrecursorsRNA Splicing
1985
The 3′ Splice Site of Pre-Messenger RNA Is Recognized by a Small Nuclear Ribonucleoprotein
Chabot B, Black D, LeMaster D, Steitz J. The 3′ Splice Site of Pre-Messenger RNA Is Recognized by a Small Nuclear Ribonucleoprotein. Science 1985, 230: 1344-1349. PMID: 2933810, DOI: 10.1126/science.2933810.Peer-Reviewed Original ResearchU2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing
Black D, Chabot B, Steitz J. U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing. Cell 1985, 42: 737-750. PMID: 2996775, DOI: 10.1016/0092-8674(85)90270-3.Peer-Reviewed Original Research
1983
Splicing of messenger RNA precursors is inhibited by antisera to small nuclear ribonucleoprotein
Padgett R, Mount S, Steitz J, Sharp P. Splicing of messenger RNA precursors is inhibited by antisera to small nuclear ribonucleoprotein. Cell 1983, 35: 101-107. PMID: 6194895, DOI: 10.1016/0092-8674(83)90212-x.Peer-Reviewed Original Research
1982
Precursor molecules of both human 5S ribosomal RNA and transfer RNAs are bound by a cellular protein reactive with anti-La Lupus antibodies
Rinke J, Steitz J. Precursor molecules of both human 5S ribosomal RNA and transfer RNAs are bound by a cellular protein reactive with anti-La Lupus antibodies. Cell 1982, 29: 149-159. PMID: 7105180, DOI: 10.1016/0092-8674(82)90099-x.Peer-Reviewed Original ResearchConceptsRRNA moleculesLa proteinLa RNAsPrecursor formNuclear transcription systemUninfected mammalian cellsPulse-chase experimentsSmall RNAsSmall ribonucleoproteinPrecursor moleculesTransfer RNAMammalian cellsRibosomal RNATRNA precursorsCertain tRNAsSpecific tRNAsRNA transcriptsTranscription systemU residuesCell extractsMature sizeHeLa cellsRNAEssential roleProtein
1980
The ribonuclease III site flanking 23S sequences in the 30S ribosomal precursor RNA of E. coli
Bram R, Young R, Steitz J. The ribonuclease III site flanking 23S sequences in the 30S ribosomal precursor RNA of E. coli. Cell 1980, 19: 393-401. PMID: 6153577, DOI: 10.1016/0092-8674(80)90513-9.Peer-Reviewed Original ResearchBase SequenceDNA, BacterialEscherichia coliNucleic Acid PrecursorsOperonRibonucleasesRNA, BacterialRNA, RibosomalAre snRNPs involved in splicing?
Lerner M, Boyle J, Mount S, Wolin S, Steitz J. Are snRNPs involved in splicing? Nature 1980, 283: 220-224. PMID: 7350545, DOI: 10.1038/283220a0.Peer-Reviewed Original ResearchConceptsSmall nuclear RNA speciesProminent nuclear proteinsRNA-protein complexesSmall RNA moleculesSmall nuclear ribonucleoproteinNuclear RNA speciesSnRNA speciesSnRNAs U1RNA speciesU6 RNASnRNA moleculesNuclear proteinsNuclear ribonucleoproteinNucleotide sequenceU1 RNARNA moleculesSnRNPsExtensive complementaritySplice junctionsNuclear locationLines of evidenceAnti-Sm seraCell nucleiHnRNA moleculesMouse Ehrlich