2013
RNA families in Epstein–Barr virus
Moss WN, Lee N, Pimienta G, Steitz JA. RNA families in Epstein–Barr virus. RNA Biology 2013, 11: 10-17. PMID: 24441309, PMCID: PMC3929418, DOI: 10.4161/rna.27488.Peer-Reviewed Original ResearchConceptsEpstein-Barr virusFunctional importanceSmall regulatory RNAsNovel Epstein-Barr virusSmall nucleolar RNAsLikely functional importanceInternal ribosomal entry siteRNA-seq studiesHuman γ-herpesvirusEvolutionary conservationNovel ncRNARegulatory RNAsShort intronsRecent bioinformaticsNucleolar RNAsOncogenic typesRNA familiesTumorigenic phenotypeStructured RNAsEBV genomeEBNA1 mRNARepetitive regionsViral latencyHigh abundanceLatency maintenance
2010
Poly(A) Tail Recognition by a Viral RNA Element Through Assembly of a Triple Helix
Mitton-Fry RM, DeGregorio SJ, Wang J, Steitz TA, Steitz JA. Poly(A) Tail Recognition by a Viral RNA Element Through Assembly of a Triple Helix. Science 2010, 330: 1244-1247. PMID: 21109672, PMCID: PMC3074936, DOI: 10.1126/science.1195858.Peer-Reviewed Original ResearchConceptsSarcoma-associated herpesvirusBox H/ACA small nucleolar RNAsMajor-groove triple helixNuclear noncoding RNANuclear retention elementSmall nucleolar RNAsViral RNA elementsRich internal loopTriple helixKaposi's sarcoma-associated herpesvirusPAN RNADeadenylation assaysRNA decayRNA clampNucleolar RNAsNoncoding RNAsNuclear RNATail recognitionRNA elementsFunctional importanceAngstrom resolutionRich loopSecondary structureRNAEne core
2003
Splicing-Dependent and -Independent Modes of Assembly for Intron-Encoded Box C/D snoRNPs in Mammalian Cells
Hirose T, Shu MD, Steitz JA. Splicing-Dependent and -Independent Modes of Assembly for Intron-Encoded Box C/D snoRNPs in Mammalian Cells. Molecular Cell 2003, 12: 113-123. PMID: 12887897, DOI: 10.1016/s1097-2765(03)00267-3.Peer-Reviewed Original ResearchConceptsBox C/D snoRNAsSplice siteSnoRNP proteinsD snoRNAsSnoRNP assemblyMammalian cellsHost intronBox C/D snoRNPsSmall nucleolar RNAsD snoRNPsRRNA modificationNucleolar RNAsHost genesActive splicingNts upstreamIntronsEfficient expressionSnoRNAsStable stemSplicingVivo analysisProteinAssemblyBlockage experimentsStem
2002
Site‐specific cross‐linking analyses reveal an asymmetric protein distribution for a box C/D snoRNP
Cahill NM, Friend K, Speckmann W, Li Z, Terns RM, Terns MP, Steitz JA. Site‐specific cross‐linking analyses reveal an asymmetric protein distribution for a box C/D snoRNP. The EMBO Journal 2002, 21: 3816-3828. PMID: 12110593, PMCID: PMC126121, DOI: 10.1093/emboj/cdf376.Peer-Reviewed Original ResearchConceptsBox C/D snoRNPsD snoRNPsC-boxBox C/D classSite-specific cross-linking analysisBox C/D small nucleolar ribonucleoproteinsBox C/DAsymmetric protein distributionSmall nucleolar ribonucleoproteinSmall nucleolar RNAsXenopus oocyte nucleusCross-linking analysisNucleolar RNAsNucleolar ribonucleoproteinD motifBox DMutational analysisOocyte nucleusGuide sequenceWidespread modificationProtein distributionFibrillarinFunctional rescueCore proteinSnoRNPs
2001
Internal Modification of U2 Small Nuclear (Snrna) Occurs in Nucleoli of Xenopus Oocytes
Yu Y, Shu M, Narayanan A, Terns R, Terns M, Steitz J. Internal Modification of U2 Small Nuclear (Snrna) Occurs in Nucleoli of Xenopus Oocytes. Journal Of Cell Biology 2001, 152: 1279-1288. PMID: 11257127, PMCID: PMC2199211, DOI: 10.1083/jcb.152.6.1279.Peer-Reviewed Original ResearchConceptsNucleolar localizationCajal bodiesU2 RNAInternal modificationSmall nuclearSm binding siteNucleolar localization signalSmall nucleolar RNAsXenopus oocytesCytoplasm of oocytesU2 small nuclearGuanosine capLocalization signalNucleolar RNAsRNAs showSubcellular sitesIntranuclear localizationIsolated nucleiRNABinding sitesNucleoliOocytesNucleotidesCytoplasmU2
1999
Guided tours: from precursor snoRNA to functional snoRNP
Weinstein L, Steitz J. Guided tours: from precursor snoRNA to functional snoRNP. Current Opinion In Cell Biology 1999, 11: 378-384. PMID: 10395551, DOI: 10.1016/s0955-0674(99)80053-2.Peer-Reviewed Original Research
1996
A mammalian gene with introns instead of exons generating stable RNA products
Tycowski K, Shu M, Steitz J. A mammalian gene with introns instead of exons generating stable RNA products. Nature 1996, 379: 464-466. PMID: 8559254, DOI: 10.1038/379464a0.Peer-Reviewed Original ResearchConceptsProtein-coding genesRibosomal subunit assemblyRibosomal RNA transcriptionSmall nucleolar RNAsMammalian genesSnoRNA genesEukaryotic cellsProtein codingMature rRNANucleolar RNAsHost genesSubunit assemblyDifferent intronsRNA transcriptionRNA productsExtensive complementarityIntronsGenesSnoRNAsExonsRNARRNATranscriptionPolysomesMaturation1
1994
Requirement for Intron-Encoded U22 Small Nucleolar RNA in 18S Ribosomal RNA Maturation
Tycowski K, Shu M, Steitz J. Requirement for Intron-Encoded U22 Small Nucleolar RNA in 18S Ribosomal RNA Maturation. Science 1994, 266: 1558-1561. PMID: 7985025, DOI: 10.1126/science.7985025.Peer-Reviewed Original ResearchConceptsRibosomal RNASmall RNAsProtein-coding gene transcriptsRibosomal RNA maturationSmall nucleolar RNAsRNA maturationVertebrate cellsCellular functionsNucleolar RNAsHost genesIntron fragmentGene transcriptsRNAXenopus oocytesU22IntronsGenesTranscriptsNucleoliOocytesMaturationTargetingCellsFragmentsDepletion
1993
A small nucleolar RNA is processed from an intron of the human gene encoding ribosomal protein S3.
Tycowski KT, Shu MD, Steitz JA. A small nucleolar RNA is processed from an intron of the human gene encoding ribosomal protein S3. Genes & Development 1993, 7: 1176-1190. PMID: 8319909, DOI: 10.1101/gad.7.7a.1176.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceCell NucleolusCell-Free SystemConserved SequenceElectrophoresis, Polyacrylamide GelHeLa CellsHumansIntronsMolecular Sequence DataNucleic Acid ConformationRestriction MappingRibosomal ProteinsRNA PrecursorsRNA Processing, Post-TranscriptionalRNA, Small NuclearSequence Analysis, RNAUracil NucleotidesConceptsSmall nucleolar RNAsNucleolar RNAsRibosomal protein S3 geneNuclear RNA polymerasesSingle-copy geneSingle primary transcriptRibosomal protein S3Secondary structure modelStem-loop structureVertebrate cellsNucleolar proteinsProtein S3Transcription signalsHuman genesRNA polymerasePrimary transcriptConserved sequencesS3 geneNucleolar snRNASame strandS3 mRNANucleotides downstreamMature endNucleolar componentsIntron 1
1989
U3, U8 and U13 comprise a new class of mammalian snRNPs localized in the cell nucleolus.
Tyc K, Steitz JA. U3, U8 and U13 comprise a new class of mammalian snRNPs localized in the cell nucleolus. The EMBO Journal 1989, 8: 3113-3119. PMID: 2531075, PMCID: PMC401391, DOI: 10.1002/j.1460-2075.1989.tb08463.x.Peer-Reviewed Original Research