2004
Guide RNAs with 5′ Caps and Novel Box C/D snoRNA-like Domains for Modification of snRNAs in Metazoa
Tycowski KT, Aab A, Steitz JA. Guide RNAs with 5′ Caps and Novel Box C/D snoRNA-like Domains for Modification of snRNAs in Metazoa. Current Biology 2004, 14: 1985-1995. PMID: 15556860, DOI: 10.1016/j.cub.2004.11.003.Peer-Reviewed Original ResearchConceptsModification guide RNAsGuide RNABox C/D snoRNAsInvariant G residueKink-turn structureGuide RNA genesShort guide RNASmall ribonucleoprotein particlesMetazoan organismsAncestral metazoanGuanosine capMetazoan cellsSingle intronD snoRNAsRNA genesSpliceosomal snRNAsTelomerase RNARibosomal RNASuch RNAsRibonucleoprotein particleMetazoansUpstream promoterIntronsG residuesCommon modification
1993
Rare scleroderma autoantibodies to the U11 small nuclear ribonucleoprotein and to the trimethylguanosine cap of U small nuclear RNAs.
Gilliam AC, Steitz JA. Rare scleroderma autoantibodies to the U11 small nuclear ribonucleoprotein and to the trimethylguanosine cap of U small nuclear RNAs. Proceedings Of The National Academy Of Sciences Of The United States Of America 1993, 90: 6781-6785. PMID: 8341699, PMCID: PMC47016, DOI: 10.1073/pnas.90.14.6781.Peer-Reviewed Original ResearchConceptsSmall nuclear ribonucleoprotein particleTrimethylguanosine capLow-abundance membersSmall nuclear RNASmall nuclear ribonucleoproteinNuclear ribonucleoprotein particleGlycerol gradient fractionsU RNAU11 small nuclear ribonucleoproteinsSnRNP complexTargeted degradationNuclear RNANuclear ribonucleoproteinRibonucleoprotein particleNuclear extractsSm classProtein componentsHeLa cellsRNPGradient fractionsRNAProteinScleroderma seraRibonucleoproteinCosedimentsUncoupling Two Functions of the U1 Small Nuclear Ribonucleoprotein Particle During in Vitro Splicing
Seiwert S, Steitz J. Uncoupling Two Functions of the U1 Small Nuclear Ribonucleoprotein Particle During in Vitro Splicing. Molecular And Cellular Biology 1993, 13: 3135-3145. DOI: 10.1128/mcb.13.6.3135-3145.1993.Peer-Reviewed Original ResearchSmall nuclear ribonucleoprotein particleSplice site recognitionU1 snRNASL RNASplice site regionSplicing substrateSpliced leader RNA sequencesVitro splicingSplice siteU1 snRNP functionsU1 small nuclear ribonucleoprotein particleEssential splicing factorLeader RNA sequencesNative gel analysisNuclear ribonucleoprotein particleSnRNP functionCaenorhabditis elegansSplicing complexesSplicing factorsLeptomonas collosomaSpliceosome assemblyDifferent intronsRibonucleoprotein particleU1 RNAMethyl oligoribonucleotideUncoupling two functions of the U1 small nuclear ribonucleoprotein particle during in vitro splicing.
Seiwert SD, Steitz JA. Uncoupling two functions of the U1 small nuclear ribonucleoprotein particle during in vitro splicing. Molecular And Cellular Biology 1993, 13: 3135-3145. PMID: 7684489, PMCID: PMC359749, DOI: 10.1128/mcb.13.6.3135.Peer-Reviewed Original ResearchMeSH KeywordsAdenoviridaeAnimalsBase SequenceCaenorhabditis elegansCell NucleusExonsHeLa CellsHumansMolecular Sequence DataNucleic Acid ConformationOligodeoxyribonucleotidesPlasmidsRibonucleoprotein, U1 Small NuclearRNARNA SplicingRNA, Small NuclearSequence Homology, Nucleic AcidTranscription, GeneticTrypanosomatinaConceptsSmall nuclear ribonucleoprotein particleU1 small nuclear ribonucleoprotein particleNuclear ribonucleoprotein particleSplice site recognitionU1 snRNASL RNASplice site regionSplicing substrateRibonucleoprotein particleSpliced leader RNA sequencesVitro splicingSplice siteU1 snRNP functionsEssential splicing factorLeader RNA sequencesNative gel analysisSnRNP functionCaenorhabditis elegansSplicing complexesSplicing factorsLeptomonas collosomaSpliceosome assemblyDifferent intronsU1 RNAMethyl oligoribonucleotide
1992
Three novel functional variants of human U5 small nuclear RNA.
Sontheimer EJ, Steitz JA. Three novel functional variants of human U5 small nuclear RNA. Molecular And Cellular Biology 1992, 12: 734-746. PMID: 1310151, PMCID: PMC364287, DOI: 10.1128/mcb.12.2.734.Peer-Reviewed Original ResearchConceptsU5 small nuclear RNASmall nuclear RNANuclear RNAHeLa cellsSmall nuclear ribonucleoprotein particleTri-snRNP complexOligonucleotide-directed RNase H cleavageNuclear ribonucleoprotein particleNovel functional variantsFull-length speciesAffinity-purified spliceosomesTrimethylguanosine capAlternative splicingShorter speciesRibonucleoprotein particleMinimal domainHeLa extractsPrimer extensionFunctional variantsHigh abundanceBase changesNorthern blottingAbundant formRNASpeciesThree Novel Functional Variants of Human U5 Small Nuclear RNA
Sontheimer E, Steitz J. Three Novel Functional Variants of Human U5 Small Nuclear RNA. Molecular And Cellular Biology 1992, 12: 734-746. DOI: 10.1128/mcb.12.2.734-746.1992.Peer-Reviewed Original ResearchU5 small nuclear RNASmall nuclear RNANuclear RNAHeLa cellsSmall nuclear ribonucleoprotein particleTri-snRNP complexOligonucleotide-directed RNase H cleavageNuclear ribonucleoprotein particleNovel functional variantsFull-length speciesAffinity-purified spliceosomesTrimethylguanosine capAlternative splicingShorter speciesRibonucleoprotein particleMinimal domainHeLa extractsPrimer extensionFunctional variantsHigh abundanceBase changesNorthern blottingAbundant formUS variantsRNA
1990
Herpesvirus saimiri U RNAs are expressed and assembled into ribonucleoprotein particles in the absence of other viral genes
Lee SI, Steitz JA. Herpesvirus saimiri U RNAs are expressed and assembled into ribonucleoprotein particles in the absence of other viral genes. Journal Of Virology 1990, 64: 3905-3915. PMID: 2164602, PMCID: PMC249686, DOI: 10.1128/jvi.64.8.3905-3915.1990.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCallitrichinaeCell NucleusCell Transformation, ViralGene ExpressionGenes, ViralHeLa CellsHerpesvirus 2, SaimiriineHumansMolecular Sequence DataNucleic Acid ConformationPlasmidsRestriction MappingRibonucleoproteinsRibonucleoproteins, Small NuclearRNA, Small NuclearT-LymphocytesTransfection
1989
The mammalian analogue of the yeast PRP8 splicing protein is present in the U4/5/6 small nuclear ribonucleoprotein particle and the spliceosome.
Pinto AL, Steitz JA. The mammalian analogue of the yeast PRP8 splicing protein is present in the U4/5/6 small nuclear ribonucleoprotein particle and the spliceosome. Proceedings Of The National Academy Of Sciences Of The United States Of America 1989, 86: 8742-8746. PMID: 2479028, PMCID: PMC298364, DOI: 10.1073/pnas.86.22.8742.Peer-Reviewed Original ResearchConceptsSmall nuclear ribonucleoproteinU5 small nuclear ribonucleoproteinHeLa cell nuclear extractsSmall nuclear ribonucleoprotein particleCell nuclear extractsAnti-trimethylguanosine antibodyNuclear ribonucleoprotein particleAffinity-purified spliceosomesSplicing proteinsMammalian proteinsYeast proteinsSnRNP complexMRNA splicingSplicing extractsRibonucleoprotein particleNuclear ribonucleoproteinMammalian analogueNuclear extractsSm classGradient fractionationSm epitopesProteinSpliceosomeProtein reactivePrp8
1987
Structural analysis of the human U3 ribonucleoprotein particle reveal a conserved sequence available for base pairing with pre-rRNA.
Parker KA, Steitz JA. Structural analysis of the human U3 ribonucleoprotein particle reveal a conserved sequence available for base pairing with pre-rRNA. Molecular And Cellular Biology 1987, 7: 2899-2913. PMID: 2959855, PMCID: PMC367909, DOI: 10.1128/mcb.7.8.2899.Peer-Reviewed Original ResearchConceptsProtein-RNA interactionsSecondary structureSubsequent reverse transcriptionRNA secondary structureHuman U3Phosphorylated proteinsRibonucleoprotein particleProcessing eventsAlternative functionsBase pairsNucleotides -159Specific nucleasesHeLa cellsProtein constituentsRNase ARNPProteinNonphosphorylated proteinsRRNAReverse transcriptionSequenceKilodaltonsNucleotides 65Specific reagentsTranscription
1984
U4 and U6 RNAs coexist in a single small nuclear ribonucleoprotein particle
Hashimoto C, Steitz J. U4 and U6 RNAs coexist in a single small nuclear ribonucleoprotein particle. Nucleic Acids Research 1984, 12: 3283-3293. PMID: 6201826, PMCID: PMC318745, DOI: 10.1093/nar/12.7.3283.Peer-Reviewed Original ResearchConceptsSmall nuclear ribonucleoproteinU6 RNARNA complexIntact small nuclear ribonucleoproteinRNA/RNA complexesHuman small nuclear ribonucleoproteinsSmall nuclear ribonucleoprotein particleRNA-RNA interactionsNuclear ribonucleoprotein particleSequence complementarityMammalian cellsNuclear ribonucleoproteinRibonucleoprotein particleSnRNP particlesRNABiological implicationsPolyacrylamide gelsProteinase KU4ComplexesSodium dodecyl sulfateRibonucleoproteinDodecyl sulfateU5U1The Ro small cytoplasmic ribonucleoproteins: identification of the antigenic protein and its binding site on the Ro RNAs.
Wolin SL, Steitz JA. The Ro small cytoplasmic ribonucleoproteins: identification of the antigenic protein and its binding site on the Ro RNAs. Proceedings Of The National Academy Of Sciences Of The United States Of America 1984, 81: 1996-2000. PMID: 6201849, PMCID: PMC345423, DOI: 10.1073/pnas.81.7.1996.Peer-Reviewed Original ResearchConceptsRibonucleoprotein particleHeLa cellsSmall cytoplasmic RNARibonuclease protection experimentsSmall ribonucleoprotein particlesSmall cytoplasmic ribonucleoproteinsHuman HeLa cellsCytoplasmic ribonucleoproteinMammalian speciesCytoplasmic RNARNA sequencesMajor proteinsRo RNAsProtection experimentsRo proteinPossible functionsBase pairsIdentical base pairsRNAProteinSystemic lupus erythematosusAntigenic proteinsAntigenic polypeptidesRo antibodiesLupus erythematosus