2018
Immunoprecipitation of Tri-methylated Capped RNA.
Hayes KE, Barr JA, Xie M, Steitz JA, Martinez I. Immunoprecipitation of Tri-methylated Capped RNA. Bio-protocol 2018, 8 PMID: 29527542, PMCID: PMC5842925, DOI: 10.21769/bioprotoc.2717.Peer-Reviewed Original ResearchPri-miRNAsCellular quiescenceAlternative miRNA biogenesis pathwaysMiRNA biogenesis pathwayNon-coding RNAsBiogenesis pathwayDNA replicationTotal RNA extractsRNA immunoprecipitationOrgan regenerationRNA extractsFibroblast cellsImmunoprecipitationRNASpecific isolationProteinQuiescenceWound healingImportant roleTrimethylguanosineMicroRNAsAutophagyCytoplasmP27Pathway
1989
[31] Determination of RNA-protein and RNA-ribonucleoprotein interactions by nuclease probing
Parker K, Steitz J. [31] Determination of RNA-protein and RNA-ribonucleoprotein interactions by nuclease probing. Methods In Enzymology 1989, 180: 454-468. PMID: 2482428, DOI: 10.1016/0076-6879(89)80117-x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodiesCarrier ProteinsElectrophoresis, Polyacrylamide GelEndoribonucleasesHumansIndicators and ReagentsKineticsMiceMicrococcal NucleaseNucleotide MappingRibonuclease HRibonuclease T1RibonucleasesRibonucleoproteinsRNARNA PrecursorsRNA SplicingRNA-Binding ProteinsRNA, Ribosomal, 28STranscription, Genetic[32] Immunoprecipitation of ribonucleoproteins using autoantibodies
Steitz J. [32] Immunoprecipitation of ribonucleoproteins using autoantibodies. Methods In Enzymology 1989, 180: 468-481. PMID: 2693908, DOI: 10.1016/0076-6879(89)80118-1.Peer-Reviewed Original ResearchConceptsSmall ribonucleoproteinImmunoprecipitation procedureTissue culture cellsRNP complexesRNA degradationLaemmli gelsRibonucleoproteinCulture cellsImmunoprecipitationWestern blottingProteinMouse ascitesRNAsCell culture supernatantsCulture supernatantsPhosphoproteinMonoclonal antibodiesRNAImmunoprecipitatesTissueRibonuclease levelsHigh backgroundMouse monoclonal autoantibodyBlottingMonoclonal autoantibodies