2021
Symmetrical arrangement of proteins under release-ready vesicles in presynaptic terminals
Radhakrishnan A, Li X, Grushin K, Krishnakumar SS, Liu J, Rothman JE. Symmetrical arrangement of proteins under release-ready vesicles in presynaptic terminals. Proceedings Of The National Academy Of Sciences Of The United States Of America 2021, 118: e2024029118. PMID: 33468631, PMCID: PMC7865176, DOI: 10.1073/pnas.2024029118.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedCryoelectron MicroscopyHippocampusImaging, Three-DimensionalMice, Inbred C57BLNerve Tissue ProteinsNeuronsPresynaptic TerminalsSynaptic VesiclesConceptsPlasma membraneSynaptic vesiclesSV fusionRelease-ready vesiclesFusion machinerySingle SNAREpinSV releaseExocytosis machineryMolecular eventsNative conditionsProtein componentsCultured hippocampal neuronsPriming reactionPresynaptic CaVesiclesFundamental processesProtein densityProtein massRelease of neurotransmittersNeurotransmitter releaseMachineryPresynaptic terminalsReleasable poolHippocampal neuronsVariable number
2020
Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System.
Chetrit D, Park D, Hu B, Liu J, Roy CR. Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System. Journal Of Visualized Experiments 2020 PMID: 32225141, DOI: 10.3791/60693.Peer-Reviewed Original ResearchConceptsDot/Icm secretion systemCryo-electron tomographySecretion systemCryo-ETDot/Icm systemDot/Icm apparatusDot/IcmSuperfolder green fluorescent proteinLive-cell imagingGreen fluorescent proteinIntact bacterial cellsPolar positioningSecretion complexPolar localizationQuantitative fluorescence microscopyBacterial poleATPase geneCytoplasmic complexDelivery of proteinsDNA substratesTiming of productionIcm systemFluorescent proteinLiving cellsBacterial cells
2011
Direct visualization of myosin-binding protein C bridging myosin and actin filaments in intact muscle
Luther PK, Winkler H, Taylor K, Zoghbi ME, Craig R, Padrón R, Squire JM, Liu J. Direct visualization of myosin-binding protein C bridging myosin and actin filaments in intact muscle. Proceedings Of The National Academy Of Sciences Of The United States Of America 2011, 108: 11423-11428. PMID: 21705660, PMCID: PMC3136262, DOI: 10.1073/pnas.1103216108.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsBiophysical PhenomenaCarrier ProteinsElectron Microscope TomographyFreeze SubstitutionHumansImaging, Three-DimensionalModels, MolecularMuscle, SkeletalMyosinsRanidae
2008
The 3D Structure of Villin as an Unusual F-Actin Crosslinker
Hampton CM, Liu J, Taylor DW, DeRosier DJ, Taylor KA. The 3D Structure of Villin as an Unusual F-Actin Crosslinker. Structure 2008, 16: 1882-1891. PMID: 19081064, PMCID: PMC2782859, DOI: 10.1016/j.str.2008.09.015.Peer-Reviewed Original ResearchMeSH KeywordsActin CytoskeletonActinsAmino Acid SequenceAnimalsBinding SitesChickensCross-Linking ReagentsImage Processing, Computer-AssistedImaging, Three-DimensionalIntestinesMicrofilament ProteinsModels, MolecularMolecular Sequence DataMolecular WeightMuscle, SkeletalProtein BindingProtein Structure, SecondaryProtein Structure, TertiaryRabbitsSequence Homology, Amino AcidTomographic subvolume alignment and subvolume classification applied to myosin V and SIV envelope spikes
Winkler H, Zhu P, Liu J, Ye F, Roux KH, Taylor KA. Tomographic subvolume alignment and subvolume classification applied to myosin V and SIV envelope spikes. Journal Of Structural Biology 2008, 165: 64-77. PMID: 19032983, PMCID: PMC2656979, DOI: 10.1016/j.jsb.2008.10.004.Peer-Reviewed Original Research