2020
Comprehensive characterization of hepatocyte-derived extracellular vesicles identifies direct miRNA-based regulation of hepatic stellate cells and DAMP-based hepatic macrophage IL-1β and IL-17 upregulation in alcoholic hepatitis mice
Eguchi A, Yan R, Pan S, Wu R, Kim J, Chen Y, Ansong C, Smith R, Tempaku M, Ohno-Machado L, Takei Y, Feldstein A, Tsukamoto H. Comprehensive characterization of hepatocyte-derived extracellular vesicles identifies direct miRNA-based regulation of hepatic stellate cells and DAMP-based hepatic macrophage IL-1β and IL-17 upregulation in alcoholic hepatitis mice. Journal Of Molecular Medicine 2020, 98: 1021-1034. PMID: 32556367, PMCID: PMC7810220, DOI: 10.1007/s00109-020-01926-7.Peer-Reviewed Original ResearchConceptsHepatic stellate cellsAlcoholic liver diseaseAlcoholic hepatitisAH miceIL-1βHepatic macrophagesStellate cellsExtracellular vesiclesPrimary hepatic stellate cellsIL-17 upregulationIL-17 productionUpregulated IL-1βHepatocyte-derived extracellular vesiclesNovel murine modelTLR9-dependent mannerMacrophage IL-1βHepatitis miceIL-17Liver diseaseControl miceCytokine productionLiver pathologyLiver fibrogenesisMurine modelΑ-SMA
2019
Human induced pluripotent stem cell-derived extracellular vesicles reduce hepatic stellate cell activation and liver fibrosis
Povero D, Pinatel E, Leszczynska A, Goyal N, Nishio T, Kim J, Kneiber D, de Araujo Horcel L, Eguchi A, Ordonez P, Kisseleva T, Feldstein A. Human induced pluripotent stem cell-derived extracellular vesicles reduce hepatic stellate cell activation and liver fibrosis. JCI Insight 2019, 5 PMID: 31184999, PMCID: PMC6675559, DOI: 10.1172/jci.insight.125652.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisCell MovementCell ProliferationChemical and Drug Induced Liver InjuryChemotaxisDisease Models, AnimalExtracellular VesiclesHepatic Stellate CellsHumansInduced Pluripotent Stem CellsLiverLiver CirrhosisMaleMiceMice, Inbred C57BLMicroRNAsTranscriptomeTransforming Growth Factor betaConceptsHepatic stellate cell activationChronic liver diseaseAnti-fibrotic effectsStellate cell activationLiver fibrosisLiver diseaseExtracellular vesiclesInduced pluripotent stem cellsCell activationDuct ligation-induced liver fibrosisFlow cytometryAnti-fibrotic approachesDevelopment of cirrhosisPro-fibrogenic responseProgression of fibrosisStem cell-derived extracellular vesiclesAnti-fibrotic strategiesCharacterization of iPSCsGreatest unmet needCell-derived extracellular vesiclesMiR-92a-3pWound healing responseStem cellsIPSC-EVsLiver injury
2017
ADAM12 induction by Twist1 promotes tumor invasion and metastasis via regulation of invadopodia and focal adhesions
Eckert M, Santiago-Medina M, Lwin T, Kim J, Courtneidge S, Yang J. ADAM12 induction by Twist1 promotes tumor invasion and metastasis via regulation of invadopodia and focal adhesions. Journal Of Cell Science 2017, 130: 2036-2048. PMID: 28468988, PMCID: PMC5482979, DOI: 10.1242/jcs.198200.Peer-Reviewed Original ResearchMeSH KeywordsADAM12 ProteinAnimalsBreast NeoplasmsCell AdhesionCell LineCell Line, TumorCell MovementCell SizeCells, CulturedEpithelial-Mesenchymal TransitionExtracellular MatrixFemaleFocal AdhesionsGene Expression Regulation, NeoplasticHEK293 CellsHumansMammary Neoplasms, AnimalMiceMutagenesis, Site-DirectedNeoplasm InvasivenessNeoplasm MetastasisNeoplasm TransplantationNuclear ProteinsProtein DomainsSignal TransductionTwist-Related Protein 1ConceptsFocal adhesionsEpithelial-mesenchymal transitionMetalloproteinase domainOverall cell adhesionFocal adhesion turnoverRegulation of invadopodiaMammary organoid culturesLive-imaging analysisTwist1 transcription factorMatrix degradationTumor invasionAdhesion turnoverPrimary tumor formationExtracellular matrix degradationInvadopodia formationTranscription factorsTranscription targetsInvadopodiaCell invasionBreast cancer cellsCell adhesionExpression levelsTumor formationPromotes Tumor InvasionCancer cellsExtracellular vesicles released by hepatocytes from gastric infusion model of alcoholic liver disease contain a MicroRNA barcode that can be detected in blood
Eguchi A, Lazaro R, Wang J, Kim J, Povero D, Willliams B, Ho S, Stärkel P, Schnabl B, Ohno‐Machado L, Tsukamoto H, Feldstein A. Extracellular vesicles released by hepatocytes from gastric infusion model of alcoholic liver disease contain a MicroRNA barcode that can be detected in blood. Hepatology 2017, 65: 475-490. PMID: 27639178, PMCID: PMC5407075, DOI: 10.1002/hep.28838.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAnalysis of VarianceAnimalsBiopsy, NeedleCells, CulturedDisease Models, AnimalExtracellular VesiclesFatty Liver, AlcoholicHepatocytesHumansImmunoblottingImmunohistochemistryMiceMicroRNAsMiddle AgedRandom AllocationReal-Time Polymerase Chain ReactionSampling StudiesSeverity of Illness IndexStatistics, NonparametricYoung AdultConceptsBlood extracellular vesiclesLiver injuryExtracellular vesiclesNuclear factor kappa BAlcoholic liver diseaseChronic liver injuryWeeks of infusionBile duct ligationB-cell lymphoma 2Levels of EVsFactor kappa BSteatohepatitis developmentNonalcoholic steatohepatitisLiver diseaseObese miceHepatic macrophagesDuct ligationKappa BMiR-340Cell originLymphoma 2Intragastric infusionMiceMiRNA signatureTime points
2011
Twist1-Induced Invadopodia Formation Promotes Tumor Metastasis
Eckert M, Lwin T, Chang A, Kim J, Danis E, Ohno-Machado L, Yang J. Twist1-Induced Invadopodia Formation Promotes Tumor Metastasis. Cancer Cell 2011, 19: 372-386. PMID: 21397860, PMCID: PMC3072410, DOI: 10.1016/j.ccr.2011.01.036.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Vesicular TransportAnimalsBase SequenceBreast NeoplasmsCell LineCell Line, TumorCell MovementCell Surface ExtensionsElectrophoresis, Polyacrylamide GelEpithelial-Mesenchymal TransitionFemaleHEK293 CellsHumansMiceMice, NudeNeoplasm MetastasisNeoplasmsNuclear ProteinsReceptor, Platelet-Derived Growth Factor alphaReverse Transcriptase Polymerase Chain ReactionRNA InterferenceSignal TransductionSrc Homology DomainsTwist-Related Protein 1ConceptsInvadopodia formationTumor metastasisFormation of invadopodiaDirect transcriptional targetTwist1 transcription factorEMT-inducing signalsMatrix degradationPromotes Tumor MetastasisMembrane protrusionsExtracellular matrix degradationTranscriptional targetsTranscription factorsInvadopodiaPDGFRα expressionMesenchymal transitionTwist1Central mediatorPDGFRαKey functionsSnail2 is an Essential Mediator of Twist1-Induced Epithelial Mesenchymal Transition and Metastasis
Casas E, Kim J, Bendesky A, Ohno-Machado L, Wolfe C, Yang J. Snail2 is an Essential Mediator of Twist1-Induced Epithelial Mesenchymal Transition and Metastasis. Cancer Research 2011, 71: 245-254. PMID: 21199805, PMCID: PMC3025803, DOI: 10.1158/0008-5472.can-10-2330.Peer-Reviewed Original Research
2010
DSGeo: Software tools for cross-platform analysis of gene expression data in GEO
Lacson R, Pitzer E, Kim J, Galante P, Hinske C, Ohno-Machado L. DSGeo: Software tools for cross-platform analysis of gene expression data in GEO. Journal Of Biomedical Informatics 2010, 43: 709-715. PMID: 20435161, PMCID: PMC2934864, DOI: 10.1016/j.jbi.2010.04.007.Peer-Reviewed Original ResearchConceptsAggregation of dataData loaderRelational databaseGene expression dataUser preferencesData browserData browsingCross-platform dataSoftware toolsSeamless integrationCross-platform analysisGroups of dataQueriesExpression dataPublic gene expression dataSpecific sample characteristicsLarge resourcesBrowserToolBrowsingAnnotatingUsersRetrievalApplicationsPlatform