2018
Asymmetric distribution and spatial switching of dynein activity generates ciliary motility
Lin J, Nicastro D. Asymmetric distribution and spatial switching of dynein activity generates ciliary motility. Science 2018, 360 PMID: 29700238, PMCID: PMC6640125, DOI: 10.1126/science.aar1968.Peer-Reviewed Original ResearchConceptsDynein activitySea urchin sperm cellsCryo-electron tomographyIndividual dyneinsDynein isoformsAsymmetric distributionMotile ciliaDyneinFlagellaHypothesis positsSperm cellsSmall populationCiliary motilityFlagellar bendingCoordinated activityActive stateMotilityActivity stateOrganellesIsoformsCiliaActivity
2014
Insights into the Structure and Function of Ciliary and Flagellar Doublet Microtubules TEKTINS, Ca2+-BINDING PROTEINS, AND STABLE PROTOFILAMENTS* ♦
Linck R, Fu X, Lin J, Ouch C, Schefter A, Steffen W, Warren P, Nicastro D. Insights into the Structure and Function of Ciliary and Flagellar Doublet Microtubules TEKTINS, Ca2+-BINDING PROTEINS, AND STABLE PROTOFILAMENTS* ♦. Journal Of Biological Chemistry 2014, 289: 17427-17444. PMID: 24794867, PMCID: PMC4067180, DOI: 10.1074/jbc.m114.568949.Peer-Reviewed Original ResearchConceptsDoublet microtubulesRibbon proteinNexin-dynein regulatory complexCiliary motilityA-tubuleIntraflagellar transportRegulatory complexAcetylated α-tubulinSignal transductionHuman homologueSpecialized functionsTektin ACalcium-binding proteinsCell organellesHuman diseasesBinding proteinΑ-tubulinΒ-tubulinSea urchin sperm flagellaSperm flagellaMicrotubule systemProteinStable protofilamentsFlagellaWide filamentsStructural mechanism of the dynein power stroke
Lin J, Okada K, Raytchev M, Smith MC, Nicastro D. Structural mechanism of the dynein power stroke. Nature Cell Biology 2014, 16: 479-485. PMID: 24727830, PMCID: PMC4102432, DOI: 10.1038/ncb2939.Peer-Reviewed Original Research
2012
The structural heterogeneity of radial spokes in cilia and flagella is conserved
Lin J, Heuser T, Carbajal‐González B, Song K, Nicastro D. The structural heterogeneity of radial spokes in cilia and flagella is conserved. Cytoskeleton 2012, 69: 88-100. PMID: 22170736, PMCID: PMC3307805, DOI: 10.1002/cm.21000.Peer-Reviewed Original ResearchConceptsFlagellar motilityRadial spokesNexin-dynein regulatory complexRegulatory complexFlagellar structureMost organismsThree-dimensional structureSubtomogram averagingCryoelectron tomographyDynein motorsDynein activityChlamydomonas flagellaMotile ciliaFlagellaSperm flagellaUbiquitous componentOrganismsFunctional differencesEssential roleCiliaRepeat unitsUnprecedented detailBasal partRS1Motility