2019
FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia
Lin J, Le TV, Augspurger K, Tritschler D, Bower R, Fu G, Perrone C, O’Toole E, Mills KV, Dymek E, Smith E, Nicastro D, Porter ME. FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia. Molecular Biology Of The Cell 2019, 30: 2659-2680. PMID: 31483737, PMCID: PMC6761771, DOI: 10.1091/mbc.e19-07-0367.Peer-Reviewed Original ResearchConceptsInner dynein armsRegulatory complexCryo-electron tomographyInner arm dyneinsCiliary motilityMultiple dynein motorsPrecise spatial organizationAxonemal repeatDocking factorUnique binding siteWD repeatsDynein assemblyAssembly factorsDomain proteinsRegulatory hubDynein complexDynein isoformsDynein subunitsInsertional mutagenesisNew lociRegulatory proteinsDynein motorsDifferent dyneinsDoublet microtubulesTransport factors
2018
Asymmetric distribution and spatial switching of dynein activity generates ciliary motility
Lin J, Nicastro D. Asymmetric distribution and spatial switching of dynein activity generates ciliary motility. Science 2018, 360 PMID: 29700238, PMCID: PMC6640125, DOI: 10.1126/science.aar1968.Peer-Reviewed Original ResearchConceptsDynein activitySea urchin sperm cellsCryo-electron tomographyIndividual dyneinsDynein isoformsAsymmetric distributionMotile ciliaDyneinFlagellaHypothesis positsSperm cellsSmall populationCiliary motilityFlagellar bendingCoordinated activityActive stateMotilityActivity stateOrganellesIsoformsCiliaActivityThe I1 dynein-associated tether and tether head complex is a conserved regulator of ciliary motility
Fu G, Wang Q, Phan N, Urbanska P, Joachimiak E, Lin J, Wloga D, Nicastro D. The I1 dynein-associated tether and tether head complex is a conserved regulator of ciliary motility. Molecular Biology Of The Cell 2018, 29: mbc.e18-02-0142. PMID: 29514928, PMCID: PMC5921572, DOI: 10.1091/mbc.e18-02-0142.Peer-Reviewed Original ResearchConceptsI1 dyneinCiliary motilityAxonemal dynein motorsDynein motor domainMotor domainCiliary doublet microtubulesCK1 kinasesProper phosphorylationNormal ciliary motilityBiochemical approachesCryoelectron tomographyDynein motorsRegulatory mechanismsMotility regulatorMotile ciliaDoublet microtubulesRegulatorDyneinStable anchoringMotilityCiliary beatingComplexesHead complexMechanical feedbackImportant role
2014
Structural mechanism of the dynein power stroke
Lin J, Okada K, Raytchev M, Smith MC, Nicastro D. Structural mechanism of the dynein power stroke. Nature Cell Biology 2014, 16: 479-485. PMID: 24727830, PMCID: PMC4102432, DOI: 10.1038/ncb2939.Peer-Reviewed Original Research
2012
One of the Nine Doublet Microtubules of Eukaryotic Flagella Exhibits Unique and Partially Conserved Structures
Lin J, Heuser T, Song K, Fu X, Nicastro D. One of the Nine Doublet Microtubules of Eukaryotic Flagella Exhibits Unique and Partially Conserved Structures. PLOS ONE 2012, 7: e46494. PMID: 23071579, PMCID: PMC3468612, DOI: 10.1371/journal.pone.0046494.Peer-Reviewed Original ResearchThe CSC connects three major axonemal complexes involved in dynein regulation
Heuser T, Dymek EE, Lin J, Smith EF, Nicastro D. The CSC connects three major axonemal complexes involved in dynein regulation. Molecular Biology Of The Cell 2012, 23: 3143-3155. PMID: 22740634, PMCID: PMC3418309, DOI: 10.1091/mbc.e12-05-0357.Peer-Reviewed Original ResearchConceptsNexin-dynein regulatory complexDynein regulationRadial spokesAxonemal complexCryo-electron tomographyWild-type motilityRegulatory complexFlagellar motilityDynein motorsChlamydomonas flagellaMotile ciliaStable assemblyFlagellaDifferent functionsRegulationRS1ComplexesMotilityStructural heterogeneityRS2Human healthImportant roleOrganellesHomologuesCSCCryoelectron tomography reveals doublet-specific structures and unique interactions in the I1 dynein
Heuser T, Barber CF, Lin J, Krell J, Rebesco M, Porter ME, Nicastro D. Cryoelectron tomography reveals doublet-specific structures and unique interactions in the I1 dynein. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: e2067-e2076. PMID: 22733763, PMCID: PMC3409752, DOI: 10.1073/pnas.1120690109.Peer-Reviewed Original ResearchConceptsI1 dyneinCryoelectron tomographyGel-based proteomicsInner arm dyneinsCiliary assemblySensory organellesSubtomogram averagingDynein activityCritical regulatorDyneinPolypeptide compositionDoublet microtubulesBiochemical comparisonMotor domainCiliary motilityFlagellar beatingUnique interactionsCiliary beatingMotilityEukaryotesAssemblyChlamydomonasSubcomplexMutantsCiliopathies
2011
Building Blocks of the Nexin-Dynein Regulatory Complex in Chlamydomonas Flagella*
Lin J, Tritschler D, Song K, Barber CF, Cobb JS, Porter ME, Nicastro D. Building Blocks of the Nexin-Dynein Regulatory Complex in Chlamydomonas Flagella*. Journal Of Biological Chemistry 2011, 286: 29175-29191. PMID: 21700706, PMCID: PMC3190725, DOI: 10.1074/jbc.m111.241760.Peer-Reviewed Original ResearchConceptsNexin-dynein regulatory complexRegulatory complexImportant regulatory nodeTwo-dimensional electrophoresisRegulatory nodesProtein phosphorylationSignal transductionPhosphorylation statusMALDI-TOF mass spectrometryDynein motorsDynein activityChlamydomonas flagellaMotile ciliaRegulatory functionsPhosphorylated isoformsCoordination of thousandsFlagellar axonemeBiochemical comparisonRadial spokesPCR analysisFlagellar bendingIsoform patternFlagellaMolecular compositionProtein