2005
Dynamic Partitioning into Lipid Rafts Controls the Endo-Exocytic Cycle of the αL/β2 Integrin, LFA-1, during Leukocyte Chemotaxis
Fabbri M, Di Meglio S, Gagliani MC, Consonni E, Molteni R, Bender JR, Tacchetti C, Pardi R. Dynamic Partitioning into Lipid Rafts Controls the Endo-Exocytic Cycle of the αL/β2 Integrin, LFA-1, during Leukocyte Chemotaxis. Molecular Biology Of The Cell 2005, 16: 5793-5803. PMID: 16207819, PMCID: PMC1289422, DOI: 10.1091/mbc.e05-05-0413.Peer-Reviewed Original ResearchConceptsDetergent-resistant membranesInternalized receptorsLate endosomal pathwayCholesterol-sequestering agentRab11 mutantDRM associationPolarized recyclingCell rearCell frontPlasma membraneChemoattractant stimulationEndocytic compartmentsEndosomal pathwayAdhesion receptorsCell migrationNew protrusionsIntegrinsLFA-1Dynamic redistributionMutantsLamellipodiaAdhesive interactionsPrimary neutrophilsIntracellular accumulationΒ2 integrins
1999
A tyrosine‐based sorting signal in the β2 integrin cytoplasmic domain mediates its recycling to the plasma membrane and is required for ligand‐supported migration
Fabbri M, Fumagalli L, Bossi G, Bianchi E, Bender J, Pardi R. A tyrosine‐based sorting signal in the β2 integrin cytoplasmic domain mediates its recycling to the plasma membrane and is required for ligand‐supported migration. The EMBO Journal 1999, 18: 4915-4925. PMID: 10487744, PMCID: PMC1171563, DOI: 10.1093/emboj/18.18.4915.Peer-Reviewed Original ResearchConceptsIntegrin cytoplasmic domainCytoplasmic domainBeta-subunit cytoplasmic domainAdhesive functionTyrosine-based endocytic signalsSubunit cytoplasmic domainClathrin-dependent endocytosisSite-directed mutagenesisNon-conservative substitutionsChinese hamster ovary cellsEndocytic signalsRegulated internalizationMembrane rufflesUnrelated functionsRecycling compartmentHamster ovary cellsEctopic expressionPlasma membraneReceptor recyclingDegradative pathwayIntegrin dynamicsCell adhesionCell migrationCell surfaceOvary cells