2002
Src Kinase Mediates Phosphatidylinositol 3-Kinase/Akt-dependent Rapid Endothelial Nitric-oxide Synthase Activation by Estrogen*
Haynes MP, Li L, Sinha D, Russell KS, Hisamoto K, Baron R, Collinge M, Sessa WC, Bender JR. Src Kinase Mediates Phosphatidylinositol 3-Kinase/Akt-dependent Rapid Endothelial Nitric-oxide Synthase Activation by Estrogen*. Journal Of Biological Chemistry 2002, 278: 2118-2123. PMID: 12431978, DOI: 10.1074/jbc.m210828200.Peer-Reviewed Original ResearchMeSH KeywordsAdenoviridaeAnimalsBlotting, WesternCell LineCells, CulturedElectrophoresis, Polyacrylamide GelEndoplasmic ReticulumEndothelium, VascularEnzyme ActivationEnzyme InhibitorsEstrogensHumansMiceMutationNitric OxideNitric Oxide SynthaseNitric Oxide Synthase Type IINitric Oxide Synthase Type IIIPhosphatidylinositol 3-KinasesPhosphorylationPrecipitin TestsProtein BindingProtein Serine-Threonine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktReceptors, EstrogenSignal TransductionSrc-Family KinasesTime FactorsTransfectionTyrosineConceptsC-SrcPI3-kinaseAkt phosphorylationSrc kinaseUpstream regulatorKinase-dead c-SrcC-Src associationActive c-SrcC-Src phosphorylationMurine embryonic fibroblastsBasal Akt phosphorylationC-Src expressionCritical upstream regulatorEndothelial nitric oxide synthaseSrc familyActive AktEmbryonic fibroblastsComplex formation resultsEndothelial cellsHuman endothelial cellsAkt activationPhosphorylationKinaseAktPhosphatidylinositol
1999
A tyrosine‐based sorting signal in the β2 integrin cytoplasmic domain mediates its recycling to the plasma membrane and is required for ligand‐supported migration
Fabbri M, Fumagalli L, Bossi G, Bianchi E, Bender J, Pardi R. A tyrosine‐based sorting signal in the β2 integrin cytoplasmic domain mediates its recycling to the plasma membrane and is required for ligand‐supported migration. The EMBO Journal 1999, 18: 4915-4925. PMID: 10487744, PMCID: PMC1171563, DOI: 10.1093/emboj/18.18.4915.Peer-Reviewed Original ResearchConceptsIntegrin cytoplasmic domainCytoplasmic domainBeta-subunit cytoplasmic domainAdhesive functionTyrosine-based endocytic signalsSubunit cytoplasmic domainClathrin-dependent endocytosisSite-directed mutagenesisNon-conservative substitutionsChinese hamster ovary cellsEndocytic signalsRegulated internalizationMembrane rufflesUnrelated functionsRecycling compartmentHamster ovary cellsEctopic expressionPlasma membraneReceptor recyclingDegradative pathwayIntegrin dynamicsCell adhesionCell migrationCell surfaceOvary cells