2005
Dynamic Partitioning into Lipid Rafts Controls the Endo-Exocytic Cycle of the αL/β2 Integrin, LFA-1, during Leukocyte Chemotaxis
Fabbri M, Di Meglio S, Gagliani MC, Consonni E, Molteni R, Bender JR, Tacchetti C, Pardi R. Dynamic Partitioning into Lipid Rafts Controls the Endo-Exocytic Cycle of the αL/β2 Integrin, LFA-1, during Leukocyte Chemotaxis. Molecular Biology Of The Cell 2005, 16: 5793-5803. PMID: 16207819, PMCID: PMC1289422, DOI: 10.1091/mbc.e05-05-0413.Peer-Reviewed Original ResearchConceptsDetergent-resistant membranesInternalized receptorsLate endosomal pathwayCholesterol-sequestering agentRab11 mutantDRM associationPolarized recyclingCell rearCell frontPlasma membraneChemoattractant stimulationEndocytic compartmentsEndosomal pathwayAdhesion receptorsCell migrationNew protrusionsIntegrinsLFA-1Dynamic redistributionMutantsLamellipodiaAdhesive interactionsPrimary neutrophilsIntracellular accumulationΒ2 integrins
1998
Posttranscriptional regulation of urokinase plasminogen activator receptor messenger RNA levels by leukocyte integrin engagement
Wang G, Collinge M, Blasi F, Pardi R, Bender J. Posttranscriptional regulation of urokinase plasminogen activator receptor messenger RNA levels by leukocyte integrin engagement. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 6296-6301. PMID: 9600959, PMCID: PMC27663, DOI: 10.1073/pnas.95.11.6296.Peer-Reviewed Original ResearchConceptsAU-rich elementsLFA-1 activationMRNA degradationReporter constructsUrokinase plasminogen activator receptorT cell activationCrucial cis-acting elementsCis-acting elementsLymphocyte function-associated antigen-1Integrin lymphocyte function-associated antigen-1Jurkat T cellsLFA-1 engagementUPAR cDNAReceptor-mediated signalingPosttranscriptional regulationTransmembrane signalingReporter mRNAIntegrin engagementCell activationInhibitor 5Adhesion receptorsSecond messengerMessenger RNA levelsPlasminogen activator receptorFunction-associated antigen-1
1996
Feedback modulation of ligand-engaged alpha L/beta 2 leukocyte integrin (LFA-1) by cyclic AMP-dependent protein kinase.
Rovere P, Inverardi L, Bender J, Pardi R. Feedback modulation of ligand-engaged alpha L/beta 2 leukocyte integrin (LFA-1) by cyclic AMP-dependent protein kinase. The Journal Of Immunology 1996, 156: 2273-9. PMID: 8690918, DOI: 10.4049/jimmunol.156.6.2273.Peer-Reviewed Original ResearchConceptsProtein kinase CAdhesion receptorsKinase CCyclic AMP-dependent protein kinaseAMP-dependent protein kinaseCytoskeletal anchoring proteinsIntegrin-dependent activationCAMP-dependent kinase activationIntracellular cAMP elevationCAMP elevationHeterologous cell linesLeukocyte integrinsAnchoring proteinsRegulated processProtein kinaseAdenylyl cyclase isoformsMolecular basisKinase activationIntercellular adhesionF-actinCell deadhesionHuman intercellular adhesion molecule-1LFA-1 receptorsDependent adhesionShort-term regulation
1995
Conserved regions in the cytoplasmic domains of the leukocyte integrin alpha L beta 2 are involved in endoplasmic reticulum retention, dimerization, and cytoskeletal association.
Pardi R, Bossi G, Inverardi L, Rovida E, Bender J. Conserved regions in the cytoplasmic domains of the leukocyte integrin alpha L beta 2 are involved in endoplasmic reticulum retention, dimerization, and cytoskeletal association. The Journal Of Immunology 1995, 155: 1252-63. PMID: 7636193, DOI: 10.4049/jimmunol.155.3.1252.Peer-Reviewed Original ResearchMeSH KeywordsAllosteric RegulationAmino Acid SequenceAnimalsCell CompartmentationCell Line, TransformedChlorocebus aethiopsCytoskeletonEndoplasmic ReticulumLymphocyte Function-Associated Antigen-1Molecular Sequence DataProtein ConformationProtein MultimerizationProtein Structure, TertiaryRecombinant Fusion ProteinsSequence DeletionT-LymphocytesTetradecanoylphorbol AcetateTransfectionConceptsAlpha L beta 2Cytoplasmic domainEndoplasmic reticulum retentionCytoskeletal associationBeta subunitAlpha L subunitAlpha beta complexFunction of integrinsBeta 2GFFKR motifAdherent cellsDeletion mutantsSubstitution mutantsEctopic expressionIntegrin alphaPlasma membraneHeterodimer formationConserved regionsAdhesion receptorsBeta complexCytoplasmic truncationRegulated functionsL subunitsStructural determinantsAlpha L