2023
Prior immunization against an intracellular antigen enhances subsequent red blood cell alloimmunization in mice
Jajosky R, Patel S, Wu S, Patel K, Covington M, Vallecillo-Zúniga M, Ayona D, Bennett A, Luckey C, Hudson K, Hendrickson J, Eisenbarth S, Josephson C, Zerra P, Stowell S, Arthur C. Prior immunization against an intracellular antigen enhances subsequent red blood cell alloimmunization in mice. Blood 2023, 141: 2642-2653. PMID: 36638335, PMCID: PMC10356576, DOI: 10.1182/blood.2022016588.Peer-Reviewed Original ResearchConceptsCD4 T cell responsesT cell responsesIntracellular antigensB cellsImmune primingRed blood cell alloimmunizationBlood cell alloantigensRate of alloimmunizationAdditional alloantibodiesAlloimmunization rateRBC alloantigensSubsequent transfusionsSame alloantigensPrior immunizationTransfusion recipientsDonor RBCsMouse modelNumerous antigensRBC antigensAlloantigensAlloimmunizationAntigenTransfusionAlloantibodiesRBCs
2021
Marginal zone B cells mediate a CD4 T-cell–dependent extrafollicular antibody response following RBC transfusion in mice
Zerra PE, Patel SR, Jajosky RP, Arthur CM, McCoy JW, Allen JWL, Chonat S, Fasano RM, Roback JD, Josephson CD, Hendrickson J, Stowell SR. Marginal zone B cells mediate a CD4 T-cell–dependent extrafollicular antibody response following RBC transfusion in mice. Blood 2021, 138: 706-721. PMID: 33876205, PMCID: PMC8394907, DOI: 10.1182/blood.2020009376.Peer-Reviewed Original ResearchConceptsMarginal zone B cellsRBC transfusionMZ B cellsB cellsHOD RBCsAlloantibody formationAntibody responseAntibody formationAntigen-specific germinal center B cellsB cell-deficient recipientsCD4 T-cell depletionRed blood cell transfusionCD4 T cell activationRBC alloantibody formationBlood cell transfusionT-cell depletionCD4 T cellsProbability of complicationsExtrafollicular antibody responsesGerminal center B cellsFollicular B cellsT cell activationRBC alloimmunizationCell transfusionSubsequent transfusions
2015
Innate and adaptive immune responses to transfused alloantigens
Zimring J, Hudson K, Hendrickson J. Innate and adaptive immune responses to transfused alloantigens. Pathology 2015, 47: s41. DOI: 10.1097/01.pat.0000461433.20240.46.Peer-Reviewed Original ResearchInnate immune activationRed blood cellsImmune activationMurine modelToll-like receptor agonistsAdaptive immune responsesExposure of recipientsRBC alloantigensSubsequent transfusionsMultiple transfusionsRBC alloantibodiesRBC transfusionTLR agonistsReceptor agonistImmune responseMouse modelTransfusionHuman studiesInnate immunityAlloimmunisationAlloantigensBlood cellsSuch exposureRecipientsAgonistsInnate and adaptive immune responses to transfused alloantigens
Zimring J, Hudson K, Hendrickson J. Innate and adaptive immune responses to transfused alloantigens. Pathology 2015, 47: s34. DOI: 10.1097/01.pat.0000461421.80336.37.Peer-Reviewed Original ResearchInnate immune activationRed blood cellsImmune activationMurine modelToll-like receptor agonistsAdaptive immune responsesExposure of recipientsRBC alloantigensSubsequent transfusionsMultiple transfusionsRBC alloantibodiesRBC transfusionTLR agonistsReceptor agonistImmune responseMouse modelTransfusionHuman studiesInnate immunityAlloimmunisationAlloantigensBlood cellsSuch exposureRecipientsAgonists
2012
Cellular Protection Against Antibodies Occurs Through a Complement-Independent Pathway
Stowell S, Liepkalns J, Hendrickson J, Pierce K, Smith N, Zimring J. Cellular Protection Against Antibodies Occurs Through a Complement-Independent Pathway. Blood 2012, 120: 3290. DOI: 10.1182/blood.v120.21.3290.3290.Peer-Reviewed Original ResearchHOD RBCsKO miceSurface C3Nonimmunized miceAntigen levelsT cellsAntibody-reactive antigensC3 KO miceComplement inhibitory factorsAutoimmune hemolytic anemiaCell-mediated destructionAntibody-mediated destructionComplement-dependent mechanismAntibody-mediated processComplement-independent pathwaysComplement-independent mechanismsAntibody-secreting cellsNonimmunized control miceComplement degradation productsAntibody engagementCellular protectionCellular resistanceHOD antigenSubsequent transfusionsAntibody levels
2011
A Novel Murine Model of Pregnancy and Transfusion Induced Anti-Kell RBC Alloimmunization
Smith N, Henry K, Zimring J, Hendrickson J. A Novel Murine Model of Pregnancy and Transfusion Induced Anti-Kell RBC Alloimmunization. Blood 2011, 118: 715. DOI: 10.1182/blood.v118.21.715.715.Peer-Reviewed Original ResearchNon-immunized mothersRBC alloimmunizationNovel murine modelGlycoprotein antibodiesC57BL/6 recipientsImmunized mothersNegative fetusMurine modelAnimal modelsC57BL/6 femalesMechanisms of immunizationRBC-specific expressionSubset of pregnanciesFlow cytometric crossmatchSubtype-specific antibodiesDays of lifeFirst animal modelAnti-Kell antibodiesPrior pregnancySubsequent transfusionsCytometric crossmatchRBC transfusionAntibody titersRepeat pregnancyRBC antibodies