2023
Associations of donor, component, and recipient factors on hemoglobin increments following red blood cell transfusion in very low birth weight infants
DeSimone R, Plimier C, Goel R, Hendrickson J, Josephson C, Patel R, Sola‐Visner M, Roubinian N. Associations of donor, component, and recipient factors on hemoglobin increments following red blood cell transfusion in very low birth weight infants. Transfusion 2023, 63: 1424-1429. PMID: 37387597, PMCID: PMC10530070, DOI: 10.1111/trf.17468.Peer-Reviewed Original ResearchConceptsLow birth weight infantsRed blood cell transfusionBirth weight infantsBlood cell transfusionVLBW infantsHemoglobin incrementsTransfusion effectivenessCell transfusionWeight infantsRBC transfusionHemoglobin levelsRecipient factorsBlood donorsSingle-unit RBC transfusionsRBC unitsAssociation of donorDonor hemoglobin levelsTransfusion episodesTransfusion eventsClinical outcomesDonor factorsMultivariable regressionTransfusionDonor sexFemale donorsClass switching is differentially regulated in RBC alloimmunization and vaccination
Prakash A, Medved J, Arneja A, Niebuhr C, Li A, Tarrah S, Boscia A, Burnett E, Singh A, Salazar J, Xu W, Santhanakrishnan M, Hendrickson J, Luckey C. Class switching is differentially regulated in RBC alloimmunization and vaccination. Transfusion 2023, 63: 826-838. PMID: 36907655, PMCID: PMC10851675, DOI: 10.1111/trf.17301.Peer-Reviewed Original ResearchConceptsSTAT6 KO miceSTAT6-deficient miceHOD RBCsRole of STAT6IgG subtypesRBC alloimmunizationKO miceDeficient miceTotal IgG responseIgG subclass distributionRBC transfusionIgG responsesWT miceAntibody responseIgG3 subclassSubclass distributionIgG subclassesMouse modelHuman patientsVaccinationMiceStudy designAltered levelsSubtypesClass switching
2022
Clodronate inhibits alloimmunization against distinct red blood cell alloantigens in mice
Arthur CM, Patel SR, Sharma A, Zerra PE, Chonat S, Jajosky RP, Fasano RM, Patel R, Bennett A, Zhou X, Luckey CJ, Hudson KE, Eisenbarth SC, Josephson CD, Roback JD, Hendrickson JE, Stowell SR. Clodronate inhibits alloimmunization against distinct red blood cell alloantigens in mice. Transfusion 2022, 62: 948-953. PMID: 35470900, PMCID: PMC9491148, DOI: 10.1111/trf.16872.Peer-Reviewed Original ResearchConceptsRBC alloimmunizationRBC transfusionAntibody formationPreclinical modelsRed blood cell transfusionBlood cell alloantigensBlood cell transfusionTransfusion of RBCsTransfusion-dependent patientsDevelopment of alloantibodiesIgG antibody formationAlloantigen exposureHOD RBCsCell transfusionPost transfusionAlloantibody formationPharmacological removalIgG antibodiesTransfusionAlloimmunizationClodronateMarginal sinusPrior treatmentDay 5KEL antigenDonor genetic and non-genetic factors affecting red blood cell transfusion effectiveness
Roubinian NH, Reese SE, Qiao H, Plimier C, Fang F, Page GP, Cable RG, Custer B, Gladwin MT, Goel R, Harris B, Hendrickson JE, Kanias T, Kleinman S, Mast AE, Sloan SR, Spencer BR, Spitalnik SL, Busch MP, Hod EA, . Donor genetic and non-genetic factors affecting red blood cell transfusion effectiveness. JCI Insight 2022, 7: e152598. PMID: 34793330, PMCID: PMC8765041, DOI: 10.1172/jci.insight.152598.Peer-Reviewed Original ResearchConceptsTransfusion effectivenessHemoglobin incrementsRBC transfusionG6PD deficiencyMulticenter retrospective studyRBC storage durationRBC unit transfusionPrecision medicine approachSubset of donorsTransfusion episodesTransfusion requirementsUnit transfusionRecipient factorsRetrospective studyRBC recipientsPatient outcomesRecipient characteristicsChild healthTransfusionVivo hemolysisTransfusion productsMedicine approachNon-genetic factorsOxidative hemolysisSingle nucleotide polymorphisms
2021
The lysophospholipid‐binding molecule CD1D is not required for the alloimmunization response to fresh or stored RBCs in mice despite RBC storage driving alterations in lysophospholipids
Medved J, Knott BM, Tarrah SN, Li AN, Shah N, Moscovich TC, Boscia AR, Salazar JE, Santhanakrishnan M, Hendrickson JE, Fu X, Zimring JC, Luckey CJ. The lysophospholipid‐binding molecule CD1D is not required for the alloimmunization response to fresh or stored RBCs in mice despite RBC storage driving alterations in lysophospholipids. Transfusion 2021, 61: 2169-2178. PMID: 34181769, PMCID: PMC8856511, DOI: 10.1111/trf.16554.Peer-Reviewed Original ResearchMeSH KeywordsAlarminsAnimalsAntibody SpecificityAntigens, CD1dBlood PreservationBlood TransfusionDuffy Blood-Group SystemErythrocytesFemaleImmunizationImmunoglobulin GImmunoglobulin MIsoantibodiesIsoantigensLysophospholipidsMaleMass SpectrometryMiceMice, Inbred StrainsMice, KnockoutMice, TransgenicMuramidaseOvalbuminReceptors, Cell SurfaceTransfusion ReactionConceptsCD1d-deficient miceCD1d deficiencyRBC alloimmunizationImmune activationNonclassical major histocompatibility complex class IWild-type control miceMajor histocompatibility complex class IHistocompatibility complex class IAdverse clinical consequencesSignificant adverse clinical consequencesLow baseline levelsRBC storageComplex class IHOD RBCsMolecule CD1dRBC transfusionWT miceControl miceImmune responseClinical consequencesMouse modelCD1dCD1d recognitionPolyclonal immunoglobulinsBaseline levelsComplement Plays a Critical Role in Inflammation-Induced Immunoprophylaxis Failure in Mice
Escamilla-Rivera V, Santhanakrishnan M, Liu J, Gibb DR, Forsmo JE, Foxman EF, Eisenbarth SC, Luckey CJ, Zimring JC, Hudson KE, Stowell SR, Hendrickson JE. Complement Plays a Critical Role in Inflammation-Induced Immunoprophylaxis Failure in Mice. Frontiers In Immunology 2021, 12: 704072. PMID: 34249009, PMCID: PMC8270673, DOI: 10.3389/fimmu.2021.704072.Peer-Reviewed Original ResearchConceptsImmunoprophylaxis failureRed blood cellsRBC transfusionComplement receptorsHuman KEL glycoproteinB cell activation thresholdWild-type micePresence of complementMurine red blood cellsTwo-hit modelRecipient inflammationIgG alloantibodiesInflammatory monocytesAdaptive immunityType miceB cellsRecipient complementTranslational relevanceKey cellsTransfusionMiceBlood cellsImmunoprophylaxis efficacyBaseline stateActivation thresholdTransfusion practices in a large cohort of hospitalized children
Nellis ME, Goel R, Hendrickson JE, Birch R, Patel RM, Karafin MS, Hanson SJ, Sachais BS, Hauser RG, Luban NLC, Gottschall J, Sola‐Visner M, Josephson CD, Karam O, Study‐IV‐Pediatric F. Transfusion practices in a large cohort of hospitalized children. Transfusion 2021, 61: 2042-2053. PMID: 33973660, DOI: 10.1111/trf.16443.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentBlood Component TransfusionBlood TransfusionChildChild, PreschoolDatasets as TopicDiagnosis-Related GroupsFemaleHospital MortalityHospitals, CommunityHospitals, TeachingHumansInfantInfant, NewbornInpatientsMaleProcedures and Techniques UtilizationRetrospective StudiesUnited StatesConceptsRBC transfusionCardiopulmonary bypassTransfusion practiceHospitalized childrenInpatient encountersBlood componentsLarge cohortHospital settingBlood Institute Recipient EpidemiologyDonor Evaluation Study-IIIPatient blood management initiativesDiagnostic categoriesFrequency of transfusionInstitutional transfusion guidelinesCohort of patientsInternational normalized ratioPercent of encountersNational Heart LungPediatric hospital settingYears of ageSubset of childrenRecipient EpidemiologyTransfusion guidelinesRed blood cellsEntire cohortMarginal zone B cells mediate a CD4 T-cell–dependent extrafollicular antibody response following RBC transfusion in mice
Zerra PE, Patel SR, Jajosky RP, Arthur CM, McCoy JW, Allen JWL, Chonat S, Fasano RM, Roback JD, Josephson CD, Hendrickson J, Stowell SR. Marginal zone B cells mediate a CD4 T-cell–dependent extrafollicular antibody response following RBC transfusion in mice. Blood 2021, 138: 706-721. PMID: 33876205, PMCID: PMC8394907, DOI: 10.1182/blood.2020009376.Peer-Reviewed Original ResearchConceptsMarginal zone B cellsRBC transfusionMZ B cellsB cellsHOD RBCsAlloantibody formationAntibody responseAntibody formationAntigen-specific germinal center B cellsB cell-deficient recipientsCD4 T-cell depletionRed blood cell transfusionCD4 T cell activationRBC alloantibody formationBlood cell transfusionT-cell depletionCD4 T cellsProbability of complicationsExtrafollicular antibody responsesGerminal center B cellsFollicular B cellsT cell activationRBC alloimmunizationCell transfusionSubsequent transfusions
2020
A potential association of an interferon gene signature with RBC alloimmunization in sickle cell disease
Madany E, Kadi N, Pandya S, Hendrickson J, Gibb D. A potential association of an interferon gene signature with RBC alloimmunization in sickle cell disease. The Journal Of Immunology 2020, 204: 145.18-145.18. DOI: 10.4049/jimmunol.204.supp.145.18.Peer-Reviewed Original ResearchSickle cell diseaseInterferon-stimulated genesRBC alloimmunizationMore alloantibodiesGene signatureSCD patientsCell diseaseFrequency of alloimmunizationChronic inflammatory stateInterferon gene signatureProduction of alloantibodiesMultiple interferon-stimulated genesType 1 interferonMyxovirus resistance protein 1Resistance protein 1Alloimmunization frequencyCompatible RBCsRBC transfusionInflammatory stateMxA expressionDisease populationTransfusion modelAlloimmunizationPatientsSiglec-1
2018
HLA Class I Alloimmunization and Platelet Transfusion Support in HLA-Identical Bone Marrow Transplant for Sickle Cell Disease: A Sickle Transplant Alliance for Research Study
Nickel R, Horan J, Abraham A, Qayed M, Haight A, Luban N, Hendrickson J. HLA Class I Alloimmunization and Platelet Transfusion Support in HLA-Identical Bone Marrow Transplant for Sickle Cell Disease: A Sickle Transplant Alliance for Research Study. Blood 2018, 132: 3816. DOI: 10.1182/blood-2018-99-114116.Peer-Reviewed Original ResearchBone marrow transplantHLA class ISickle cell diseaseHLA-identical bone marrow transplantsMore platelet transfusionsNon-alloimmunized patientsPlatelet transfusion supportPlatelet transfusionsRBC transfusionClass IPositive PRAPre-BMTTransfusion supportPediatric patientsMarrow transplantCell diseaseBone marrowRed blood cell transfusionHepatic sinusoidal obstruction syndromeApheresis platelet transfusionHLA-disparate donorsBlood cell transfusionSinusoidal obstruction syndromePlatelet transfusion requirementsCord blood grafts6 A Novel Association Between High Red Blood Cell Alloimmunization Rates and Hereditary Hemorrhagic Telangiectasia (HHT)
Zheng Y, Pollak J, Henderson K, Hendrickson J, Tormey C. 6 A Novel Association Between High Red Blood Cell Alloimmunization Rates and Hereditary Hemorrhagic Telangiectasia (HHT). American Journal Of Clinical Pathology 2018, 149: s166-s166. DOI: 10.1093/ajcp/aqx149.375.Peer-Reviewed Original ResearchHereditary hemorrhagic telangiectasiaAlloimmunization rateRBC transfusionHHT patientsHemorrhagic telangiectasiaRed blood cell transfusionFirst RBC transfusionMore RBC transfusionsNon-ABO alloantibodiesProphylactic antigen matchingRBC alloimmunization rateBlood cell transfusionMultiple arteriovenous malformationsStudent's t-testAutosomal dominant disorderCommon alloantibodiesHHT subjectsCell transfusionReferral centerControl patientsMale patientsAntigen matchingRetrospective studyAntibody screenPatient population
2017
Red blood cell alloimmunisation: induction of immunity and potential mitigation strategies
Hendrickson J. Red blood cell alloimmunisation: induction of immunity and potential mitigation strategies. ISBT Science Series 2017, 13: 105-111. DOI: 10.1111/voxs.12360.Peer-Reviewed Original ResearchAnimal modelsChronic inflammatory disease statesRBC unitsAntigen-specific toleranceT-cell markersInduction of immunityInflammatory disease statesHumoral immune responseHumoral immune responsivenessAlloantibody developmentRBC alloimmunisationDonor antigensDendritic cellsRBC alloantibodiesRBC transfusionTransfusion recipientsImmune responsivenessRBC exposureRisk factorsMurine studiesForeign antigensImmune responseCD 4Danger signalsImmune system
2016
Type 1 Interferon Regulates Inflammation Associated RBC Alloimmunization By Promoting Monocyte-Derived Dendritic Cell Erythrophagocytosis in Mice
Gibb D, Natarajan P, Liu J, Santhanakrishnan M, Iwasaki A, Hendrickson J. Type 1 Interferon Regulates Inflammation Associated RBC Alloimmunization By Promoting Monocyte-Derived Dendritic Cell Erythrophagocytosis in Mice. Blood 2016, 128: 19. DOI: 10.1182/blood.v128.22.19.19.Peer-Reviewed Original ResearchMonocyte-derived dendritic cellsReactivity of seraPeak antibody responseWT miceIfnar1-/- miceType 1 interferonDendritic cellsRBC transfusionPeripheral bloodAntibody responseRBC alloimmunizationAlloimmune responseSpecific IgGIgG antibodiesInflammatory diseasesMouse modelRBC antigensIndividual miceCCR2-/- miceFrequency of alloimmunizationHuman KEL glycoproteinFlow cytometric crossmatchActivation marker expressionDendritic cell activationRole of inflammation
2015
Antigen Density Impacts RBC Survival and Antigen Modulation Following Incompatible RBC Transfusion
Yoo J, Arthur C, Zerra P, Girard-Pierce K, Hendrickson J, Stowell S. Antigen Density Impacts RBC Survival and Antigen Modulation Following Incompatible RBC Transfusion. Blood 2015, 126: 2350. DOI: 10.1182/blood.v126.23.2350.2350.Peer-Reviewed Original ResearchIncompatible RBC transfusionHemolytic transfusion reactionsWild-type C57BL/6 miceRBC transfusionIncompatible transfusionsAntigen densityAntigen modulationFlow cytometric analysisKEL antigenRBC survivalKEL RBCsImmunized recipientsAntigen levelsCytometric analysisFatal hemolytic transfusion reactionRecipient WT miceChronic transfusion therapyTransfusion of RBCsNon-immunized miceLow antigen densityAntibody-mediated removalAlloimmunized individualsAdverse eventsDonor antigensPost transfusionA Novel Network Analysis Tool to Identify Relationships Between Disease States and Risk for RBC Alloimmunization
Celli R, Schulz W, Hendrickson J, Tormey C. A Novel Network Analysis Tool to Identify Relationships Between Disease States and Risk for RBC Alloimmunization. Blood 2015, 126: 2349. DOI: 10.1182/blood.v126.23.2349.2349.Peer-Reviewed Original ResearchDisease statesControl cohortAcute myeloid leukemia/myelodysplastic syndromeLeukemia/myelodysplastic syndromeRed blood cell alloimmunizationChronic transfusion supportHyper-responder groupAcute renal failureAML/MDSAcute myocardial infarctionICD-9 codesDevelopment of alloantibodiesSpecific medical conditionsElectronic medical recordsSignificant disease associationCertain disease statesAlloimmunized individualsRBC alloimmunizationGastrointestinal bleedPulmonary hypertensionRenal failureTransfusion burdenTransfusion supportMale patientsRBC transfusionAnti-RhD Mediates Loss of RhD Antigen Following Anti-RhD Infusion
Sullivan H, Arthur C, Patel S, Hendrickson J, Lazarus A, Stowell S. Anti-RhD Mediates Loss of RhD Antigen Following Anti-RhD Infusion. Blood 2015, 126: 3570. DOI: 10.1182/blood.v126.23.3570.3570.Peer-Reviewed Original ResearchDirect antiglobulin testAutoimmune hemolytic anemiaRed blood cellsAntigen lossAntigen levelsRhD antigenHemolytic anemiaPost-infusion samplesTarget antigenAntigen detectionRhD-positive red blood cellsK antigenIncompatible RBC transfusionIdiopathic thrombocytopenic purpuraAnti-RhD antibodiesHospital blood bankSignificant complement activationImmune globulinRBC transfusionThrombocytopenic purpuraAntiglobulin testMurine modelAntibody injectionRBC clearanceDetectable antigenInnate and adaptive immune responses to transfused alloantigens
Zimring J, Hudson K, Hendrickson J. Innate and adaptive immune responses to transfused alloantigens. Pathology 2015, 47: s41. DOI: 10.1097/01.pat.0000461433.20240.46.Peer-Reviewed Original ResearchInnate immune activationRed blood cellsImmune activationMurine modelToll-like receptor agonistsAdaptive immune responsesExposure of recipientsRBC alloantigensSubsequent transfusionsMultiple transfusionsRBC alloantibodiesRBC transfusionTLR agonistsReceptor agonistImmune responseMouse modelTransfusionHuman studiesInnate immunityAlloimmunisationAlloantigensBlood cellsSuch exposureRecipientsAgonistsInnate and adaptive immune responses to transfused alloantigens
Zimring J, Hudson K, Hendrickson J. Innate and adaptive immune responses to transfused alloantigens. Pathology 2015, 47: s34. DOI: 10.1097/01.pat.0000461421.80336.37.Peer-Reviewed Original ResearchInnate immune activationRed blood cellsImmune activationMurine modelToll-like receptor agonistsAdaptive immune responsesExposure of recipientsRBC alloantigensSubsequent transfusionsMultiple transfusionsRBC alloantibodiesRBC transfusionTLR agonistsReceptor agonistImmune responseMouse modelTransfusionHuman studiesInnate immunityAlloimmunisationAlloantigensBlood cellsSuch exposureRecipientsAgonists
2014
Reticuloendothelial Saturation Dictates the Development of RBC Resistance to Antibody-Mediated Clearance Following Incompatible Transfusion
Arthur C, Mener A, Patel S, Rodrigues L, Xue C, Nickel R, Zimring J, Hendrickson J, Stowell S. Reticuloendothelial Saturation Dictates the Development of RBC Resistance to Antibody-Mediated Clearance Following Incompatible Transfusion. Blood 2014, 124: 1559. DOI: 10.1182/blood.v124.21.1559.1559.Peer-Reviewed Original ResearchIncompatible RBC transfusionRed blood cellsRBC transfusionInitial transfusionRBC clearanceAntigen modulationOld red blood cellsAntibody-mediated removalFlow cytometric analysisHOD antigenIncompatible transfusionsDetectable antigenRBC ageReticuloendothelial systemCytometric analysisPreferential clearanceAntibody-Mediated ClearanceRBC-specific antibodiesAntibody-opsonized cellsPhagocytic removalTransfused cellsC57BL/6 recipientsAntigen levelsFVB miceRecipient mice
2013
KEL RBC Transfusion Induces IgG Anti-KEL Antibodies Independent Of CD4 T Cells
Stowell S, Girard-Pierce K, Arthur C, Smith N, Zimring J, Hendrickson J. KEL RBC Transfusion Induces IgG Anti-KEL Antibodies Independent Of CD4 T Cells. Blood 2013, 122: 41. DOI: 10.1182/blood.v122.21.41.41.Peer-Reviewed Original ResearchCD4 T cellsMHC class IIRBC alloantibody formationRBC transfusionT cellsKEL RBCsIsotype controlAlloantibody formationC57BL/6 recipientsRBC alloantibodiesRBC alloantigensClass IICD-4 T-cellsCD4 T-cell depletionRed blood cell transfusionCD4 T cell helpMHC class II antigensChronic transfusion therapyBlood cell transfusionT-cell depletionHemolytic transfusion reactionsIsotype control antibodyClass II antigensT cell helpMHC antigen presentation