2023
Storage differentially impacts alloimmunization to distinct red cell antigens following transfusion in mice
Maier C, Jajosky R, Patel S, Verkerke H, Fuller M, Allen J, Zerra P, Fasano R, Chonat S, Josephson C, Gibb D, Eisenbarth S, Luckey C, Hudson K, Hendrickson J, Arthur C, Stowell S. Storage differentially impacts alloimmunization to distinct red cell antigens following transfusion in mice. Transfusion 2023, 63: 457-462. PMID: 36708051, PMCID: PMC10414794, DOI: 10.1111/trf.17251.Peer-Reviewed Original ResearchConceptsKEL RBCsAntibody formationAntigen levelsRed blood cell alloimmunizationIgG antibody productionDifferent clinical outcomesIgG antibody formationRed cell antigensAlloantibody productionRBC alloimmunizationClinical outcomesTransfusionAlloimmunizationRBC clearanceCell antigensClinical experienceSpecific antigenAntibody productionRBC antigensRBC survivalAntibody developmentModel antigenAntigenAdditional studiesFresh RBCsPrior immunization against an intracellular antigen enhances subsequent red blood cell alloimmunization in mice
Jajosky R, Patel S, Wu S, Patel K, Covington M, Vallecillo-Zúniga M, Ayona D, Bennett A, Luckey C, Hudson K, Hendrickson J, Eisenbarth S, Josephson C, Zerra P, Stowell S, Arthur C. Prior immunization against an intracellular antigen enhances subsequent red blood cell alloimmunization in mice. Blood 2023, 141: 2642-2653. PMID: 36638335, PMCID: PMC10356576, DOI: 10.1182/blood.2022016588.Peer-Reviewed Original ResearchConceptsCD4 T cell responsesT cell responsesIntracellular antigensB cellsImmune primingRed blood cell alloimmunizationBlood cell alloantigensRate of alloimmunizationAdditional alloantibodiesAlloimmunization rateRBC alloantigensSubsequent transfusionsSame alloantigensPrior immunizationTransfusion recipientsDonor RBCsMouse modelNumerous antigensRBC antigensAlloantigensAlloimmunizationAntigenTransfusionAlloantibodiesRBCs
2018
1 Type I Interferon Is Necessary and Sufficient for Alloimmunization to Transfused KEL-Expressing RBCs in Mice
Gibb D, Liu J, Natarajan P, Santhanakrishnan M, Madrid D, Eisenbarth S, Zimring J, Iwasaki A, Hendrickson J. 1 Type I Interferon Is Necessary and Sufficient for Alloimmunization to Transfused KEL-Expressing RBCs in Mice. American Journal Of Clinical Pathology 2018, 149: s163-s163. DOI: 10.1093/ajcp/aqx149.370.Peer-Reviewed Original ResearchIFNα/βAlloimmune responseType I interferonKEL RBCsRBC alloimmunizationWT miceIFNAR1 expressionInflammatory stimuliB cellsI interferonChimeric miceRBC antigensNew transgenic mouse modelCertain inflammatory disordersHuman KEL glycoproteinRed blood cell antigensIFNα/β productionToll-like receptorsInterferon regulatory factor 3Transgenic mouse modelBlood cell antigensRegulatory factor 3Non-hematopoietic cellsIgG alloantibodiesTransfusion protocol
2016
Type 1 Interferon Regulates Inflammation Associated RBC Alloimmunization By Promoting Monocyte-Derived Dendritic Cell Erythrophagocytosis in Mice
Gibb D, Natarajan P, Liu J, Santhanakrishnan M, Iwasaki A, Hendrickson J. Type 1 Interferon Regulates Inflammation Associated RBC Alloimmunization By Promoting Monocyte-Derived Dendritic Cell Erythrophagocytosis in Mice. Blood 2016, 128: 19. DOI: 10.1182/blood.v128.22.19.19.Peer-Reviewed Original ResearchMonocyte-derived dendritic cellsReactivity of seraPeak antibody responseWT miceIfnar1-/- miceType 1 interferonDendritic cellsRBC transfusionPeripheral bloodAntibody responseRBC alloimmunizationAlloimmune responseSpecific IgGIgG antibodiesInflammatory diseasesMouse modelRBC antigensIndividual miceCCR2-/- miceFrequency of alloimmunizationHuman KEL glycoproteinFlow cytometric crossmatchActivation marker expressionDendritic cell activationRole of inflammation
2013
Transfusion Of RBCs With Low-Density KEL Induces Tolerance To The KEL Antigen
Stowell S, Arthur C, Smith N, Girard-Pierce K, Zimring J, Hendrickson J. Transfusion Of RBCs With Low-Density KEL Induces Tolerance To The KEL Antigen. Blood 2013, 122: 1160. DOI: 10.1182/blood.v122.21.1160.1160.Peer-Reviewed Original ResearchKEL RBCsRBC transfusionAntibody formationKEL antigenDetectable IgMRBC alloimmunizationWestern blot analysisC57BL/6 recipientsAntigen densityDay 3RBC antigensDays post transfusionTransfusion of RBCsSubsequent responseBlot analysisFlow cytometric analysisAnalysis of serumCompatible RBCsRBC alloantigensRepeat transfusionPost transfusionAntigen levelsImmunological outcomesRBC exposureSpecific alloantibodies
2012
Marginal Zone B Cells Mediate Alloantibody Formation to a Clinically Significant Human RBC Antigen in a Murine Model
Girard-Pierce K, Stowell S, Smith N, Henry K, Zimring J, Hendrickson J. Marginal Zone B Cells Mediate Alloantibody Formation to a Clinically Significant Human RBC Antigen in a Murine Model. Blood 2012, 120: 843. DOI: 10.1182/blood.v120.21.843.843.Peer-Reviewed Original ResearchMZ B cellsHemolytic transfusion reactionsTransfusion of RBCsC57BL/6 recipient miceRecipient miceAlloimmune responseB cellsRBC antigensRBC alloimmunizationAlloantibody responsesPost transfusionMouse modelRBC clearanceFlow cytometryNovel transgenic mouse modelBlood borne antigensConsequences of alloimmunizationControl recipient miceDays post transfusionHuman KEL glycoproteinRBC-specific expressionB-cell depletionRed blood cell antigensAntibody-mediated immunityMarginal zone B cells
2009
Leukoreduction Decreases Alloimmunogenicity of Transfused Murine HOD RBCs.
Hendrickson J, Hod E, Spitalnik S, Hillyer C, Zimring J. Leukoreduction Decreases Alloimmunogenicity of Transfused Murine HOD RBCs. Blood 2009, 114: 640. DOI: 10.1182/blood.v114.22.640.640.Peer-Reviewed Original ResearchWhite blood cellsHOD RBCsPost-transfusion survivalCytometric crossmatchingRBC alloimmunizationLeukoreduction filtersHen egg lysozymeRBC antigensFlow cytometryRBC-specific expressionRed blood cell antigensReductionist murine modelHemolytic transfusion reactionsMinority of miceLateral tail veinAntigen hen egg lysozymeBlood cell antigensEffect of leukoreductionResidual white blood cellsPropridium iodideAltered clearanceHLA alloimmunizationSingle transfusionHLA antigensTransfusion reactions
2008
A Novel Model of Autoimmunity to Self Red Blood Cell Antigens
Hendrickson J, Cadwell C, Hillyer C, Zimring J. A Novel Model of Autoimmunity to Self Red Blood Cell Antigens. Blood 2008, 112: 3461. DOI: 10.1182/blood.v112.11.3461.3461.Peer-Reviewed Original ResearchAutoimmune hemolytic anemiaB1 B cellsSelf-reactive B cellsReactive B cellsAutoreactive B cellsB cell toleranceB cellsControl micePositive miceB cell maturationLymph nodesBone marrowCell toleranceRBC antigensCell maturationRBC-specific expressionRed blood cell antigensSystemic immune activationHuman blood group antigensAntigen hen egg lysozymeLife-threatening illnessConventional B cellsBlood cell antigensSigns of illnessHemoglobin breakdown products
2006
Recipient Inflammation That Increases Alloimmunization Also Enhances Consumption of Transfused RBCs by Dendritic Cells.
Hendrickson J, Roback J, Hillyer C, Zimring J. Recipient Inflammation That Increases Alloimmunization Also Enhances Consumption of Transfused RBCs by Dendritic Cells. Blood 2006, 108: 23. DOI: 10.1182/blood.v108.11.23.23.Peer-Reviewed Original ResearchLiver dendritic cellsDendritic cellsT cellsRBC antigensRed blood cellsImmune responseSplenic macrophagesB cellsRBC consumptionCo-stimulatory molecule expressionBlood cellsRed blood cell antigensMajority of patientsC57BL/6 recipient micePresence of inflammationAbsence of inflammationRole of antigenLymph node macrophagesBlood cell antigensRBC alloimmunizationRecipient inflammationAlloantibody responsesLymph nodesRBC transfusionPotent APCsLack of Alloantibody Response to Red Blood Cell Antigens in Juvenile Mice Following Transfusion: Non-Immunogenic or Tolerogenic Response?.
Hendrickson J, Josephson C, Chadwick T, Zimring J. Lack of Alloantibody Response to Red Blood Cell Antigens in Juvenile Mice Following Transfusion: Non-Immunogenic or Tolerogenic Response?. Blood 2006, 108: 955. DOI: 10.1182/blood.v108.11.955.955.Peer-Reviewed Original ResearchRed blood cellsAlloantibody responsesJuvenile miceAdult miceRBC antigensPotential clinical implicationsRBC alloimmunizationClinical implicationsWeight-adjusted volumeRed blood cell antigensEffects of inflammationBlood cell antigensMonths of ageOngoing studiesViral inflammationTolerogenic responsesIgG levelsPediatric populationTransfusion recipientsImmunogenic stimulusPatient populationNeonatal humansTolerogenic stimulusMurine modelAlloimmunization