2023
A CUG-initiated CATSPERθ functions in the CatSper channel assembly and serves as a checkpoint for flagellar trafficking
Huang X, Miyata H, Wang H, Mori G, Iida-Norita R, Ikawa M, Percudani R, Chung J. A CUG-initiated CATSPERθ functions in the CatSper channel assembly and serves as a checkpoint for flagellar trafficking. Proceedings Of The National Academy Of Sciences Of The United States Of America 2023, 120: e2304409120. PMID: 37725640, PMCID: PMC10523455, DOI: 10.1073/pnas.2304409120.Peer-Reviewed Original ResearchConceptsChannel assemblySperm flagellaTransmembrane domain-containing proteinsSperm tail formationDomain-containing proteinsCatSper channel complexMale fertilityFlagellar traffickingMacromolecular complexesTail formationPhysiological roleSuccessful fertilizationCalcium signalingCatSper channelsFlagellaGenetic abrogationChannel complexNormal expressionDimer formationSpermatid cellsProteinCheckpointHyperactivated motilityAssemblyPotential role
2022
3D structure and in situ arrangements of CatSper channel in the sperm flagellum
Zhao Y, Wang H, Wiesehoefer C, Shah NB, Reetz E, Hwang JY, Huang X, Wang TE, Lishko PV, Davies KM, Wennemuth G, Nicastro D, Chung JJ. 3D structure and in situ arrangements of CatSper channel in the sperm flagellum. Nature Communications 2022, 13: 3439. PMID: 35715406, PMCID: PMC9205950, DOI: 10.1038/s41467-022-31050-8.Peer-Reviewed Original ResearchConceptsCatSper complexSperm flagellaCryo-electron tomographyHigher-order organizationMammalian spermIntracellular domainStructural basisExtracellular domainTetrameric channelChannel CatSperSuccessful fertilizationFlagellar movementCatSper channelsChannel poreSitu mapSitu arrangementHuman sperm flagellumPrimary Ca2CatSperDimer formationFlagellaCentral roleSpermAtomic modelSperm motilityCatSper and its CaM‐like Ca2+ sensor EFCAB9 are necessary for the path chirality of sperm
Wiesehöfer C, Wiesehöfer M, Dankert JT, Chung J, von Ostau N, Singer BB, Wennemuth G. CatSper and its CaM‐like Ca2+ sensor EFCAB9 are necessary for the path chirality of sperm. The FASEB Journal 2022, 36: e22288. PMID: 35438819, PMCID: PMC9835897, DOI: 10.1096/fj.202101656rr.Peer-Reviewed Original ResearchConceptsNNC 55Exposure of spermMotility changesPharmacological inhibitionLateral head excursionCatSper activationIntracellular CaPhysiological stimuliCatSper channelsProtein 2Zona pellucida protein 2Zona pellucida glycoproteinsCapacitation stateCatSperHead excursionMouse spermPellucida glycoproteinsSpermSuccessful fertilizationMibefradilHigh frequency
2020
3D in situ imaging of female reproductive tract reveals molecular signatures of fertilizing spermatozoa in mice
Ded L, Hwang JY, Miki K, Shi HF, Chung JJ. 3D in situ imaging of female reproductive tract reveals molecular signatures of fertilizing spermatozoa in mice. ELife 2020, 9: e62043. PMID: 33078708, PMCID: PMC7707823, DOI: 10.7554/elife.62043.Peer-Reviewed Original ResearchConceptsFemale reproductive tractFemale tractCalcium channel complexSitu molecular imagingReproductive tractMolecular insightsSuccessful fertilizationCatSper channelsMolecular signaturesSperm cellsSperm selectionChannel complexMouse spermatozoaFertilization siteSpermSperm migrationSpermatozoaMammalsPhosphorylationCatSper1ProteinSitu imagingAcrosomeUtero-tubal junctionSelection
2011
A novel gene required for male fertility and functional CATSPER channel formation in spermatozoa
Chung JJ, Navarro B, Krapivinsky G, Krapivinsky L, Clapham DE. A novel gene required for male fertility and functional CATSPER channel formation in spermatozoa. Nature Communications 2011, 2: 153. PMID: 21224844, PMCID: PMC3999383, DOI: 10.1038/ncomms1153.Peer-Reviewed Original ResearchHyperactivation of motilityCatSper channel complexMale fertilityChannel α-subunitsNovel subunitNovel genesHyperactivated motilitySperm proteinsGene productsChannel assemblyAuxiliary subunitsΑ-subunitSuccessful fertilizationCalcium signalingSelective poresSpermatozoa lackChannel functionSubunitsSperm Ca2Channel complexCation channelsGenesChannel formationProteinAcrosome reaction