A CRISPR Competition Assay to Identify Cancer Genetic Dependencies.
Girish V, Sheltzer JM. A CRISPR Competition Assay to Identify Cancer Genetic Dependencies. Bio-protocol 2020, 10: e3682. PMID: 33659353, PMCID: PMC7842800, DOI: 10.21769/bioprotoc.3682.Peer-Reviewed Original ResearchGenetic dependenciesTargeted locusCancer cell fitnessCRISPR/Cas9 systemGene of interestWhole-genome screenAnti-cancer drug developmentCell fitnessNuclease Cas9Mammalian cellsGenome editingGene dependenciesCas9 systemSpecific genesConsequences of lossCompetition assaysCell linesPotential targetGenesLociCancer typesDrug developmentFunction perturbationsCas9CRISPR