2006
Antibody-mediated Hsp70 protein therapy
Hansen JE, Sohn W, Kim C, Chang SS, Huang NC, Santos DG, Chan G, Weisbart RH, Nishimura RN. Antibody-mediated Hsp70 protein therapy. Brain Research 2006, 1088: 187-196. PMID: 16630585, DOI: 10.1016/j.brainres.2006.03.025.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBlotting, WesternCell SurvivalCells, CulturedCerebral CortexChlorocebus aethiopsDose-Response Relationship, DrugHSP70 Heat-Shock ProteinsHumansHydrogen PeroxideImmunoglobulin Variable RegionNeuronsOxidantsProtein TransportRatsRats, WistarRecombinant Fusion ProteinsTransduction, GeneticTransfectionConceptsPrimary rat cortical neuronsRat cortical neuronsCortical neuronsIntracellular HSP70 levelsCOS-7 cellsSingle-chain Fv fragmentAnti-DNA antibodiesProtein therapyIntracellular HSP70Stressful stimuliFv fragmentMAb 3E10Hsp70 levelsNeuronsTherapyProtein transduction domainSubsequent exposureCellsHuman diseasesNovel protein transduction domainFirst evidenceTransduction domainLiving cellsHSP70Fusion proteinRole of p21-activated kinase pathway defects in the cognitive deficits of Alzheimer disease
Zhao L, Ma QL, Calon F, Harris-White ME, Yang F, Lim GP, Morihara T, Ubeda OJ, Ambegaokar S, Hansen JE, Weisbart RH, Teter B, Frautschy SA, Cole GM. Role of p21-activated kinase pathway defects in the cognitive deficits of Alzheimer disease. Nature Neuroscience 2006, 9: 234-242. PMID: 16415866, DOI: 10.1038/nn1630.Peer-Reviewed Original ResearchConceptsAlzheimer's diseaseCognitive deficitsDrebrin lossMental retardationP21-activated kinase pathwaysTransgenic mouse modelDendritic spine morphogenesisPotential causal roleP21-activated kinaseHippocampal neuronsDendritic spinesMouse modelΒ-amyloidAdult miceMemory impairmentDiseaseProtein drebrinSpine morphogenesisCausal roleCofilin pathologyDeficitsPAK pathwayPathologyPAK inhibitionKinase pathway
2005
Antibody Mediated Transduction of Therapeutic Proteins into Living Cells
Hansen JE, Weisbart RH, Nishimura RN. Antibody Mediated Transduction of Therapeutic Proteins into Living Cells. The Scientific World JOURNAL 2005, 5: 782-788. PMID: 16170440, PMCID: PMC5936519, DOI: 10.1100/tsw.2005.98.Commentaries, Editorials and LettersMeSH KeywordsAnimalsAutoantibodiesBiological Transport, ActiveCells, CulturedDrug Delivery SystemsEndocytosisGene Products, tatHIV-1HumansImmunoglobulin FragmentsLymphokinesMicePeptide FragmentsProtein TransportProteinsSialoglycoproteinsSignal TransductionTat Gene Products, Human Immunodeficiency VirusConceptsProtein transduction domainTransduction domainNovel protein transduction domainTherapeutic proteinsReceptor-mediated endocytosisSuch proteinsHIV-1 Tat proteinCell-penetrating peptidesMAb 3E10Active proteinLiving cellsCell membraneProteinTat proteinProtein therapyCellsSingle-chain fragmentTherapeutic peptidesRecent studiesFv fragmentDomainFragmentsDirect deliveryEndocytosisTransduction