2010
Recombinant Fv-Hsp70 Protein Mediates Neuroprotection After Focal Cerebral Ischemia in Rats
Zhan X, Ander BP, Liao IH, Hansen JE, Kim C, Clements D, Weisbart RH, Nishimura RN, Sharp FR. Recombinant Fv-Hsp70 Protein Mediates Neuroprotection After Focal Cerebral Ischemia in Rats. Stroke 2010, 41: 538-543. PMID: 20075343, PMCID: PMC2957177, DOI: 10.1161/strokeaha.109.572537.Peer-Reviewed Original ResearchConceptsFocal cerebral ischemiaCerebral ischemiaInfarction volumeSensorimotor functionSaline-treated control groupIntraluminal suture techniqueExperimental ischemic strokeExperimental stroke modelsMiddle cerebral arteryOnset of ischemiaInfarct volumeIschemic brainIschemic strokeCerebral arteryFocal ischemiaControl brainsStroke modelTail veinIschemiaControl groupSuture techniqueWestern blotEndogenous Hsp70RatsBrain
2008
p21-activated Kinase-aberrant Activation and Translocation in Alzheimer Disease Pathogenesis*
Ma QL, Yang F, Calon F, Ubeda OJ, Hansen JE, Weisbart RH, Beech W, Frautschy SA, Cole GM. p21-activated Kinase-aberrant Activation and Translocation in Alzheimer Disease Pathogenesis*. Journal Of Biological Chemistry 2008, 283: 14132-14143. PMID: 18347024, PMCID: PMC2376243, DOI: 10.1074/jbc.m708034200.Peer-Reviewed Original ResearchConceptsCultured hippocampal neuronsAlzheimer's diseaseHippocampal neuronsDendritic spinesTransgenic miceAlzheimer's disease brainAlzheimer's amyloid pathologyAlzheimer's disease (AD) pathogenesisBeta-amyloid oligomersOligomer treatmentSrc family tyrosine kinase inhibitor PP2P-NR2BSynaptic deficitsAmyloid pathologyDisease brainTyrosine kinase inhibitor PP2Disease pathogenesisCognitive deficitsMental retardation syndromeProtein drebrinKinase inhibitor PP2DiseaseNeuronsRetardation syndromeMice
2006
Antibody-mediated Hsp70 protein therapy
Hansen JE, Sohn W, Kim C, Chang SS, Huang NC, Santos DG, Chan G, Weisbart RH, Nishimura RN. Antibody-mediated Hsp70 protein therapy. Brain Research 2006, 1088: 187-196. PMID: 16630585, DOI: 10.1016/j.brainres.2006.03.025.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBlotting, WesternCell SurvivalCells, CulturedCerebral CortexChlorocebus aethiopsDose-Response Relationship, DrugHSP70 Heat-Shock ProteinsHumansHydrogen PeroxideImmunoglobulin Variable RegionNeuronsOxidantsProtein TransportRatsRats, WistarRecombinant Fusion ProteinsTransduction, GeneticTransfectionConceptsPrimary rat cortical neuronsRat cortical neuronsCortical neuronsIntracellular HSP70 levelsCOS-7 cellsSingle-chain Fv fragmentAnti-DNA antibodiesProtein therapyIntracellular HSP70Stressful stimuliFv fragmentMAb 3E10Hsp70 levelsNeuronsTherapyProtein transduction domainSubsequent exposureCellsHuman diseasesNovel protein transduction domainFirst evidenceTransduction domainLiving cellsHSP70Fusion proteinRole of p21-activated kinase pathway defects in the cognitive deficits of Alzheimer disease
Zhao L, Ma QL, Calon F, Harris-White ME, Yang F, Lim GP, Morihara T, Ubeda OJ, Ambegaokar S, Hansen JE, Weisbart RH, Teter B, Frautschy SA, Cole GM. Role of p21-activated kinase pathway defects in the cognitive deficits of Alzheimer disease. Nature Neuroscience 2006, 9: 234-242. PMID: 16415866, DOI: 10.1038/nn1630.Peer-Reviewed Original ResearchConceptsAlzheimer's diseaseCognitive deficitsDrebrin lossMental retardationP21-activated kinase pathwaysTransgenic mouse modelDendritic spine morphogenesisPotential causal roleP21-activated kinaseHippocampal neuronsDendritic spinesMouse modelΒ-amyloidAdult miceMemory impairmentDiseaseProtein drebrinSpine morphogenesisCausal roleCofilin pathologyDeficitsPAK pathwayPathologyPAK inhibitionKinase pathway