2012
A Cell-Penetrating Bispecific Antibody for Therapeutic Regulation of Intracellular Targets
Weisbart RH, Gera JF, Chan G, Hansen JE, Li E, Cloninger C, Levine AJ, Nishimura RN. A Cell-Penetrating Bispecific Antibody for Therapeutic Regulation of Intracellular Targets. Molecular Cancer Therapeutics 2012, 11: 2169-2173. PMID: 22863609, DOI: 10.1158/1535-7163.mct-12-0476-t.Peer-Reviewed Original ResearchConceptsBispecific antibodiesBispecific single-chain Fv fragmentsMonoclonal antibody 3G5Cancer therapyDelivery of antibodiesIntracellular targetsMolecular therapyTherapeutic useTherapeutic regulationMonoclonal antibodiesTherapyAntibodiesMAb 3E10Single-chain Fv fragmentInhibits growthP53 levelsImportant targetMDM2Limited accessFv fragmentTargetTumors
2006
Antibody-mediated Hsp70 protein therapy
Hansen JE, Sohn W, Kim C, Chang SS, Huang NC, Santos DG, Chan G, Weisbart RH, Nishimura RN. Antibody-mediated Hsp70 protein therapy. Brain Research 2006, 1088: 187-196. PMID: 16630585, DOI: 10.1016/j.brainres.2006.03.025.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBlotting, WesternCell SurvivalCells, CulturedCerebral CortexChlorocebus aethiopsDose-Response Relationship, DrugHSP70 Heat-Shock ProteinsHumansHydrogen PeroxideImmunoglobulin Variable RegionNeuronsOxidantsProtein TransportRatsRats, WistarRecombinant Fusion ProteinsTransduction, GeneticTransfectionConceptsPrimary rat cortical neuronsRat cortical neuronsCortical neuronsIntracellular HSP70 levelsCOS-7 cellsSingle-chain Fv fragmentAnti-DNA antibodiesProtein therapyIntracellular HSP70Stressful stimuliFv fragmentMAb 3E10Hsp70 levelsNeuronsTherapyProtein transduction domainSubsequent exposureCellsHuman diseasesNovel protein transduction domainFirst evidenceTransduction domainLiving cellsHSP70Fusion protein
2005
Antibody Mediated Transduction of Therapeutic Proteins into Living Cells
Hansen JE, Weisbart RH, Nishimura RN. Antibody Mediated Transduction of Therapeutic Proteins into Living Cells. The Scientific World JOURNAL 2005, 5: 782-788. PMID: 16170440, PMCID: PMC5936519, DOI: 10.1100/tsw.2005.98.Commentaries, Editorials and LettersMeSH KeywordsAnimalsAutoantibodiesBiological Transport, ActiveCells, CulturedDrug Delivery SystemsEndocytosisGene Products, tatHIV-1HumansImmunoglobulin FragmentsLymphokinesMicePeptide FragmentsProtein TransportProteinsSialoglycoproteinsSignal TransductionTat Gene Products, Human Immunodeficiency VirusConceptsProtein transduction domainTransduction domainNovel protein transduction domainTherapeutic proteinsReceptor-mediated endocytosisSuch proteinsHIV-1 Tat proteinCell-penetrating peptidesMAb 3E10Active proteinLiving cellsCell membraneProteinTat proteinProtein therapyCellsSingle-chain fragmentTherapeutic peptidesRecent studiesFv fragmentDomainFragmentsDirect deliveryEndocytosisTransductionAn intracellular delivery vehicle for protein transduction of micro-dystrophin
Weisbart RH, Hansen JE, Nishimura RN, Chan G, Wakelin R, Chang SS, Baresi L, Chamberlain JS. An intracellular delivery vehicle for protein transduction of micro-dystrophin. Journal Of Drug Targeting 2005, 13: 81-87. PMID: 15823959, DOI: 10.1080/10611860400029002.Peer-Reviewed Original Research
2004
Antibody-mediated transduction of p53 selectively kills cancer cells.
Weisbart RH, Hansen JE, Chan G, Wakelin R, Chang SS, Heinze E, Miller CW, Koeffler PH, Yang F, Cole GM, Min YS, Nishimura RN. Antibody-mediated transduction of p53 selectively kills cancer cells. International Journal Of Oncology 2004, 25: 1867-73. PMID: 15547728, DOI: 10.3892/ijo.25.6.1867.Peer-Reviewed Original ResearchConceptsCancer cellsWild-type p53Expression of MDM2Cancer cell linesTransduction of p53Potential efficacyCertain cancersNon-specific toxicityFunctional defectsHuman cancersP53Cell linesCancerResult of mutationsGene therapyPrimary cellsViral vectorsCellsVivoNuclear exclusionTherapyFusion proteinFv fragmentProtein transductionAntibodies