Ablation of SYK Kinase from Expanded Primary Human NK Cells via CRISPR/Cas9 Enhances Cytotoxicity and Cytokine Production.
Dahlvang J, Dick J, Sangala J, Kennedy P, Pomeroy E, Snyder K, Moushon J, Thefaine C, Wu J, Hamilton S, Felices M, Miller J, Walcheck B, Webber B, Moriarity B, Hart G. Ablation of SYK Kinase from Expanded Primary Human NK Cells via CRISPR/Cas9 Enhances Cytotoxicity and Cytokine Production. The Journal Of Immunology 2023, 210: 1108-1122. PMID: 36881874, PMCID: PMC10073313, DOI: 10.4049/jimmunol.2200488.Peer-Reviewed Original ResearchConceptsAb-dependent cellular cytotoxicityAdaptive NK cellsPrimary human NK cellsNK cellsHuman NK cellsCytokine productionFcRγ chainSHP-1Loss of sykTarget cell conjugationNK cell phenotypeReduced cytokine productionTNF-α productionCRISPR/Cas9 systemPhosphatase SHP-1Transcription factor PLZFCell conjugationCellular cytotoxicityImmune stateCD2 expressionSyk expressionLack expressionEnhances CytotoxicityCas9 systemEnhanced cytotoxicity