2001
Persistent infection in Neotoma fuscipes (Muridae: Sigmodontinae) with Ehrlichia phagocytophila sensu lato.
Castro M, Nicholson W, Kramer V, Childs J. Persistent infection in Neotoma fuscipes (Muridae: Sigmodontinae) with Ehrlichia phagocytophila sensu lato. American Journal Of Tropical Medicine And Hygiene 2001, 65: 261-7. PMID: 11693866, DOI: 10.4269/ajtmh.2001.65.261.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, BacterialArachnid VectorsBase SequenceBorrelia burgdorferiCaliforniaDisease ReservoirsDNA, BacterialEhrlichiaEhrlichiosisFemaleFluorescent Antibody Technique, IndirectHeat-Shock ProteinsHumansIxodesMaleMicePolymerase Chain ReactionRatsRodent DiseasesSeasonsSeroepidemiologic StudiesSigmodontinaeZoonosesConceptsPolymerase chain reaction testingGroESL heat shock operonPersistent infectionReaction testingRatsE. phagocytophilaLyme diseaseBorrelia burgdorferiMiceSeropositive animalsDusky-footed wood ratsPCRWood ratsSeroreversionSensu latoSeroconversionSeropositiveBiopsySerologySeroreactivityInfectionDiseaseBloodAdult Ixodes pacificus CooleySerum
2000
Experimental evidence of host specificity of Bartonella infection in rodents
Kosoy M, Saito E, Green D, Marston E, Jones D, Childs J. Experimental evidence of host specificity of Bartonella infection in rodents. Comparative Immunology Microbiology And Infectious Diseases 2000, 23: 221-238. PMID: 11038125, DOI: 10.1016/s0147-9571(99)00075-2.Peer-Reviewed Original ResearchDETECTION OF ANTIBODIES REACTIVE WITH EHRLICHIA CHAFFEENSIS IN THE RACCOON
Comer J, Nicholson W, Paddock C, Sumner J, Childs J. DETECTION OF ANTIBODIES REACTIVE WITH EHRLICHIA CHAFFEENSIS IN THE RACCOON. Journal Of Wildlife Diseases 2000, 36: 705-712. PMID: 11085432, DOI: 10.7589/0090-3558-36.4.705.Peer-Reviewed Original ResearchSurvival of Ehrlichia chaffeensis in refrigerated, ADSOL‐treated RBCs
McKechnie D, Slater K, Childs J, Massung R, Paddock C. Survival of Ehrlichia chaffeensis in refrigerated, ADSOL‐treated RBCs. Transfusion 2000, 40: 1041-1047. PMID: 10988303, DOI: 10.1046/j.1537-2995.2000.40091041.x.Peer-Reviewed Original ResearchConceptsE. chaffeensisNucleated cellsTransfusion-acquired infectionEhrlichia chaffeensisMonocytic cell line THP-1Cell line THP-1Infected cell linesCytocentrifuged slidesTransfusion transmissionPacked RBC unitsPCR positivityE. chaffeensis DNASupernatant fractionCell culturesWeekly examinationsRBC unitsStudy designRBC productsTHP-1DH82 cellsPCR resultsCell linesChaffeensisInfectionSuccessful reisolationPrimary Isolation of Ehrlichia chaffeensis from Patients with Febrile Illnesses: Clinical and Molecular Characteristics
Standaert S, Yu T, Scott M, Childs J, Paddock C, Nicholson W, Singleton J, Blaser M. Primary Isolation of Ehrlichia chaffeensis from Patients with Febrile Illnesses: Clinical and Molecular Characteristics. The Journal Of Infectious Diseases 2000, 181: 1082-1088. PMID: 10720534, DOI: 10.1086/315346.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAgedChildChild, PreschoolEhrlichia chaffeensisEhrlichiosisFemaleFeverHumansInfantMaleMiddle AgedPolymerase Chain ReactionConceptsPolymerase chain reactionE. chaffeensisCerebrospinal fluid specimensCultures of bloodHigh diagnostic yieldEhrlichia chaffeensisPrimary isolationFebrile illnessDiagnostic titerBlood isolatesDiagnostic yieldTick exposureSame patientCerebrospinal fluidPatientsFluid specimensDiagnostic testsFrequent isolationBloodChain reactionChaffeensisInfectionMolecular characteristicsMolecular analysisIsolates
1999
Population Dynamics of a Naturally Occurring Heterogeneous Mixture of Borrelia burgdorferiClones
Hofmeister E, Glass G, Childs J, Persing D. Population Dynamics of a Naturally Occurring Heterogeneous Mixture of Borrelia burgdorferiClones. Infection And Immunity 1999, 67: 5709-5716. PMID: 10531219, PMCID: PMC96945, DOI: 10.1128/iai.67.11.5709-5716.1999.Peer-Reviewed Original ResearchConceptsC3H/HeJ miceC.B-17 miceLarval Ixodes scapularis ticksMajority of miceB. burgdorferiInfected nymphal ticksIxodes scapularis ticksBurgdorferi antibodiesSecondary challengeHeJ micePrimary isolatesSequential isolatesC3H miceSequential infectionMiceMonoclonal antibodiesBorrelia burgdorferiC expressionScapularis ticksClonal dominanceUnique isolatesMacrorestriction analysisOspC proteinsClonal groupsEnzootic siteDusky-Footed Wood Rats (Neotoma fuscipes) as Reservoirs of Granulocytic Ehrlichiae (Rickettsiales: Ehrlichieae) in Northern California
Nicholson W, Castro M, Kramer V, Sumner J, Childs J. Dusky-Footed Wood Rats (Neotoma fuscipes) as Reservoirs of Granulocytic Ehrlichiae (Rickettsiales: Ehrlichieae) in Northern California. Journal Of Clinical Microbiology 1999, 37: 3323-3327. PMID: 10488199, PMCID: PMC85556, DOI: 10.1128/jcm.37.10.3323-3327.1999.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCaliforniaDisease ReservoirsDNA, ViralEhrlichiaHumansIxodesMaleMolecular Sequence DataPeromyscusPolymerase Chain ReactionRatsSigmodontinaeConceptsBlood samplesBlood specimensDusky-footed wood ratsConcurrent high prevalenceSeronegative blood samplesHuman granulocytic ehrlichiosisGroESL heat shock operonGranulocytic ehrlichiaePCR-positive animalsPCR positivityHigh prevalencePCR-positive ticksSeropositive specimensRat blood samplesRatsGranulocytic ehrlichiosisEhrlichial DNAPacificus adultsEhrlichia equiEhrlichial agentsMicePCR assaysAdultsWood ratsRodentsOutcome of Diagnostic Tests Using Samples from Patients with Culture-Proven Human Monocytic Ehrlichiosis: Implications for Surveillance
Childs J, Sumner J, Nicholson W, Massung R, Standaert S, Paddock C. Outcome of Diagnostic Tests Using Samples from Patients with Culture-Proven Human Monocytic Ehrlichiosis: Implications for Surveillance. Journal Of Clinical Microbiology 1999, 37: 2997-3000. PMID: 10449489, PMCID: PMC85432, DOI: 10.1128/jcm.37.9.2997-3000.1999.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, BacterialEhrlichia chaffeensisEhrlichiosisFluorescent Antibody Technique, IndirectHumansImmunoglobulin GImmunoglobulin MPolymerase Chain ReactionConceptsHuman monocytic ehrlichiosisDiagnosis of HMEConvalescent-phase serum samplesMonocytic ehrlichiosisDetection of morulaeInitial clinical presentationCulture-proven casesE. chaffeensis antigenWhole blood specimensCompatible illnessClinical presentationInitial presentationSerologic responseCase definitionHME patientsBlood specimensImmunoglobulin MPatientsBlood smearsIndirect immunofluorescenceEhrlichial DNADiagnostic testsSerum samplesProbable diseaseEhrlichia chaffeensisMolecular Cloning and Characterization of the Ehrlichia chaffeensis Variable-Length PCR Target: an Antigen-Expressing Gene That Exhibits Interstrain Variation
Sumner J, Childs J, Paddock C. Molecular Cloning and Characterization of the Ehrlichia chaffeensis Variable-Length PCR Target: an Antigen-Expressing Gene That Exhibits Interstrain Variation. Journal Of Clinical Microbiology 1999, 37: 1447-1453. PMID: 10203503, PMCID: PMC84798, DOI: 10.1128/jcm.37.5.1447-1453.1999.Peer-Reviewed Original ResearchConceptsOpen reading frameAmino acid sequenceApparent molecular massE. chaffeensisGenomic libraryMolecular cloningReading frameAcid sequenceDifferentiation of isolatesPCR targetsClose homologyMolecular massNucleotide sequencingImmunoreactive proteinDifferent isolatesDNA samplesInterstrain variationGenesRepeat unitsMolecular databasesCell culturesAmblyomma americanum ticksSequencePCR detectionChaffeensisLongitudinal study of infection with Borrelia burgdorferi in a population of Peromyscus leucopus at a Lyme disease-enzootic site in Maryland.
Hofmeister E, Ellis B, Glass G, Childs J. Longitudinal study of infection with Borrelia burgdorferi in a population of Peromyscus leucopus at a Lyme disease-enzootic site in Maryland. American Journal Of Tropical Medicine And Hygiene 1999, 60: 598-609. PMID: 10348235, DOI: 10.4269/ajtmh.1999.60.598.Peer-Reviewed Original ResearchConceptsPolymerase chain reactionB. burgdorferiJuvenile miceCross-sectional sampleEar tissueBorrelia burgdorferi infectionIxodes scapularis ticksInfected miceSusceptible miceChronic infectionOverall prevalenceIncidence rateBurgdorferi infectionHigh riskLower riskInfectionMiceInfected ticksLongitudinal sampleBorrelia burgdorferiScapularis ticksChain reactionBurgdorferiLongitudinal studyIndirect transmissionDiagnosis of Human Ehrlichiosis by PCR Assay of Acute-Phase Serum
Comer J, Nicholson W, Sumner J, Olson J, Childs J. Diagnosis of Human Ehrlichiosis by PCR Assay of Acute-Phase Serum. Journal Of Clinical Microbiology 1999, 37: 31-34. PMID: 9854059, PMCID: PMC84159, DOI: 10.1128/jcm.37.1.31-34.1999.Peer-Reviewed Original ResearchMeSH KeywordsAcute-Phase ReactionDNA, BacterialEhrlichia chaffeensisEhrlichiosisHumansPolymerase Chain ReactionRNA, Ribosomal, 16SConceptsDetermination of etiologyAcute-phase seraHuman granulocytic ehrlichiosisSerologic testingHuman ehrlichiosisAcute-phase serum samplesEarly detectionWhole blood specimensPCR assaysBlood specimensPCR testingEtiologic agentGranulocytic ehrlichiosisEtiologyHGE agentSimilar titersSerum samplesExposure historyTitersEhrlichia chaffeensisEhrlichiosisFourfold differenceE. chaffeensisSerumAntibodiesIdentity of Ehrlichial DNA Sequences Derived from Ixodes ricinus Ticks with Those Obtained from Patients with Human Granulocytic Ehrlichiosis in Slovenia
Petrovec M, Sumner J, Nicholson W, Childs J, Strle F, Barlič J, Lotrič-Furlan S, AvšičŽupanc T. Identity of Ehrlichial DNA Sequences Derived from Ixodes ricinus Ticks with Those Obtained from Patients with Human Granulocytic Ehrlichiosis in Slovenia. Journal Of Clinical Microbiology 1999, 37: 209-210. PMID: 9854093, PMCID: PMC84210, DOI: 10.1128/jcm.37.1.209-210.1999.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDNA, BacterialEhrlichiaEhrlichiosisGenes, InsectHeat-Shock ProteinsHumansIxodesPolymerase Chain ReactionRNA, Ribosomal, 16SSlovenia
1998
An epidemiologic and entomologic investigation of a cluster of Rocky Mountain spotted fever cases in Delaware.
Rotz L, Callejas L, McKechnie D, Wolfe D, Gaw E, Hathcock L, Childs J. An epidemiologic and entomologic investigation of a cluster of Rocky Mountain spotted fever cases in Delaware. Delaware Medical Journal 1998, 70: 285-91. PMID: 9662871.Peer-Reviewed Original ResearchConceptsEntomologic investigationsMore symptomsTick-borne illnessCases of RMSFHealth care providersSerologic evaluationMedian ageRMSF casesClinical aspectsCare providersFever casesDelaware DivisionDisease controlFeverPatientsSymptomsTwo-week periodPrevention measuresPublic healthIllnessRiskRMSFHeadacheAttendeesYearsSerologic Evidence of Infection with Ehrlichia spp. in Wild Rodents (Muridae: Sigmodontinae) in the United States
Nicholson W, Muir S, Sumner J, Childs J. Serologic Evidence of Infection with Ehrlichia spp. in Wild Rodents (Muridae: Sigmodontinae) in the United States. Journal Of Clinical Microbiology 1998, 36: 695-700. PMID: 9508298, PMCID: PMC104611, DOI: 10.1128/jcm.36.3.695-700.1998.Peer-Reviewed Original Research
1997
Isolation and characterization of Ehrlichia chaffeensis strains from patients with fatal ehrlichiosis
Paddock C, Sumner J, Shore G, Bartley D, Elie R, McQuade J, Martin C, Goldsmith C, Childs J. Isolation and characterization of Ehrlichia chaffeensis strains from patients with fatal ehrlichiosis. Journal Of Clinical Microbiology 1997, 35: 2496-2502. PMID: 9316896, PMCID: PMC229999, DOI: 10.1128/jcm.35.10.2496-2502.1997.Peer-Reviewed Original ResearchConceptsE. chaffeensisDH82 cellsEhrlichia chaffeensisArkansas isolateGroESL heat shock operonFatal ehrlichiosisMechanisms of diseasePulmonary insufficiencyClinical presentationMarked thrombocytopeniaSevere diseaseEhrlichial infectionCharacteristic manifestationsPatientsDays postinoculationMolecular evaluationChaffeensisPrimary isolationRRNA gene sequencesDiseaseIsolatesSpecific associationNew isolatesGene sequencesProtein gene
1995
EAR BIOPSY LOCATION INFLUENCES DETECTION OF BORRELIA BURGDORFERI BY PCR, BUT NOT BY CULTURE IN NATURALLY INFECTED PEROMYSCUS LEUCOPUS
Hofmeister E, Childs J. EAR BIOPSY LOCATION INFLUENCES DETECTION OF BORRELIA BURGDORFERI BY PCR, BUT NOT BY CULTURE IN NATURALLY INFECTED PEROMYSCUS LEUCOPUS. Journal Of Wildlife Diseases 1995, 31: 345-351. PMID: 8592355, DOI: 10.7589/0090-3558-31.3.345.Peer-Reviewed Original ResearchConceptsEar biopsy samplesPolymerase chain reactionBiopsy samplesBiopsy locationB. burgdorferiBorrelia burgdorferiInfected micePeripheral samplesIndividual miceInfection statusMore tissue samplesMiceTissue samplesHigh concordanceChain reactionOrgan cultureBurgdorferiGreater frequencyEar samplesWhite-footed miceEnzootic areaEarCentral sampleIsolation of black creek canal virus, a new hantavirus from Sigmodon hispidus in Florida
Rollin P, Ksiazek T, Elliott L, Ravkov E, Martin M, Morzunov S, Livingstone W, Monroe M, Glass G, Ruo S, Khan A, Childs J, Nichol S, Peters C. Isolation of black creek canal virus, a new hantavirus from Sigmodon hispidus in Florida. Journal Of Medical Virology 1995, 46: 35-39. PMID: 7623004, DOI: 10.1002/jmv.1890460108.Peer-Reviewed Original ResearchConceptsBlack Creek Canal virusReverse transcriptase-polymerase chain reaction assaysNew hantavirusCotton ratsTranscriptase-polymerase chain reaction assaysVero E6 cellsHantavirus pulmonary syndrome casesVirologic studiesChain reaction assaysHantavirus antigensHigh seroprevalenceSyndrome casesE6 cellsSpleen homogenatesRT-PCRReaction assaysDirect RT-PCRVirusSerologic relationshipHantavirusesRatsRodentsSigmodon hispidusDade CountySeroprevalence
1994
Susceptibility of white-tailed deer (Odocoileus virginianus) to infection with Ehrlichia chaffeensis, the etiologic agent of human ehrlichiosis
Dawson J, Stallknecht D, Howerth E, Warner C, Biggie K, Davidson W, Lockhart J, Nettles V, Olson J, Childs J. Susceptibility of white-tailed deer (Odocoileus virginianus) to infection with Ehrlichia chaffeensis, the etiologic agent of human ehrlichiosis. Journal Of Clinical Microbiology 1994, 32: 2725-2728. PMID: 7852563, PMCID: PMC264150, DOI: 10.1128/jcm.32.11.2725-2728.1994.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceDeerDisease ReservoirsEhrlichia chaffeensisEhrlichiosisFemaleHumansMaleMolecular Sequence DataPolymerase Chain ReactionConceptsHuman ehrlichiosisE. canisE. chaffeensisEtiologic agentAbsence of seroconversionEhrlichia chaffeensisMacrophage-type cellsE. chaffeensis infectionLymph nodesPeripheral bloodClinical diseaseDay 10InfectionEhrlichia canisEhrlichiosisAntibodiesWhite-tailed deerChaffeensisNegative controlCanisReservoir hostsCompetent reservoirsWeeksTick vectorSeroconversionIsolation of the causative agent of hantavirus pulmonary syndrome.
Elliott L, Ksiazek T, Rollin P, Spiropoulou C, Morzunov S, Monroe M, Goldsmith C, Humphrey C, Zaki S, Krebs J, Maupin G, Gage K, Childs J, Nichol S, Peters C. Isolation of the causative agent of hantavirus pulmonary syndrome. American Journal Of Tropical Medicine And Hygiene 1994, 51: 102-8. PMID: 8059907, DOI: 10.4269/ajtmh.1994.51.102.Peer-Reviewed Original ResearchConceptsHantavirus pulmonary syndromePulmonary syndromeAcute respiratory illnessReverse transcriptase-polymerase chain reactionCausative agentTypical morphologic featuresConvalescent-phase seraTranscriptase-polymerase chain reactionUnknown hantavirusRespiratory illnessLung tissueHPS patientsVirus antigenEtiologic agentHigh mortalityMorphologic featuresIndirect immunofluorescenceGenus HantavirusVero cellsInfected cellsChain reactionRecent outbreakCell linesSyndromeDeer miceSerologic and Genetic Identification of Peromyscus maniculatus as the Primary Rodent Reservoir for a New Hantavirus in the Southwestern United States
Childs J, Ksiazek T, Spiropoulou C, Krebs J, Morzunov S, Maupin G, Gage K, Rollin P, Sarisky J, Enscore R, Frey J, Peters C, Nichol S. Serologic and Genetic Identification of Peromyscus maniculatus as the Primary Rodent Reservoir for a New Hantavirus in the Southwestern United States. The Journal Of Infectious Diseases 1994, 169: 1271-1280. PMID: 8195603, DOI: 10.1093/infdis/169.6.1271.Peer-Reviewed Original Research