2016
CFTR-associated ligand is a negative regulator of Mrp2 expression
Li M, Soroka CJ, Harry K, Boyer JL. CFTR-associated ligand is a negative regulator of Mrp2 expression. American Journal Of Physiology - Cell Physiology 2016, 312: c40-c46. PMID: 27834195, PMCID: PMC5283898, DOI: 10.1152/ajpcell.00100.2016.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsCarrier ProteinsCells, CulturedChlorocebus aethiopsCOS CellsDown-RegulationGene Expression RegulationGolgi Matrix ProteinsHepatocytesHumansMaleMembrane ProteinsMembrane Transport ProteinsMiceMultidrug Resistance-Associated Protein 2Multidrug Resistance-Associated ProteinsRatsRats, Sprague-DawleySignal TransductionConceptsPull-down assaysGST pull-down assaysCOOH-terminal PDZNegative regulatorCotransfected COS-7 cellsGlutathione S-transferase fusion proteinS-transferase fusion proteinATP-binding cassette (ABC) transportersTrans-Golgi networkCystic fibrosis transmembrane conductance regulatorProtein-protein interactionsExchanger regulatory factor 1Fibrosis transmembrane conductance regulatorStreptavidin pull-down assaysTransmembrane conductance regulatorCOS-7 cellsRegulatory factor 1PDZ domainCell surface expressionPosttranscriptional regulationTransmembrane proteinPlasma membraneLLC-PK1 cellsCassette transportersCOS-7
2012
A C‐terminal tyrosine‐based motif in the bile salt export pump directs clathrin‐dependent endocytosis
Lam P, Xu S, Soroka CJ, Boyer JL. A C‐terminal tyrosine‐based motif in the bile salt export pump directs clathrin‐dependent endocytosis. Hepatology 2012, 55: 1901-1911. PMID: 22161577, PMCID: PMC3319652, DOI: 10.1002/hep.25523.Peer-Reviewed Original ResearchConceptsTyrosine-based motifNucleotide-binding domainEndocytic motifTransmembrane segmentsCanonical tyrosine-based motifTyrosine-based endocytic motifCytoplasmic nucleotide-binding domainsClathrin-dependent pathwayClathrin-dependent endocytosisRegulation of traffickingBroad substrate specificitySite-directed mutagenesisATP-dependent transportHEK293T cellsCytoplasmic terminiEndocytosis motifCassette proteinSubsequent sequence analysisTerminal tailSubstrate specificityConstitutive internalizationReporter systemSequence analysisBile salt export pumpTerminal end