1999
Characterization of ion transport mechanisms involved in bombesin-stimulated biliary secretion in rat cholangiocytes
Cho W, Boyer J. Characterization of ion transport mechanisms involved in bombesin-stimulated biliary secretion in rat cholangiocytes. Journal Of Hepatology 1999, 30: 1045-1051. PMID: 10406182, DOI: 10.1016/s0168-8278(99)80258-x.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAcetazolamideAnimalsBarium CompoundsBileBile DuctsBombesinCarbonic Anhydrase InhibitorsChloride ChannelsChloridesIn Vitro TechniquesIon TransportMaleMicroscopy, VideoNitrobenzoatesPotassium ChannelsRatsRats, Sprague-DawleyTetraethylammonium
1995
Isolation of small polarized bile duct units.
Mennone A, Alvaro D, Cho W, Boyer J. Isolation of small polarized bile duct units. Proceedings Of The National Academy Of Sciences Of The United States Of America 1995, 92: 6527-6531. PMID: 7541542, PMCID: PMC41551, DOI: 10.1073/pnas.92.14.6527.Peer-Reviewed Original ResearchMeSH Keywords1-Methyl-3-isobutylxanthine4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAnimalsBicarbonatesBile DuctsBucladesineChloridesCholestasisColforsinFluoresceinsGluconatesHydrogen-Ion ConcentrationKineticsLiverMaleMicroscopy, ElectronMicroscopy, VideoRatsRats, Sprague-DawleySecretinTime FactorsConceptsBile duct unitsInterlobular bile ductsBile ductDuct unitsSmall interlobular bile ductsBile duct syndromeBile duct segmentsCholestatic liver disordersCl-/HCO3- exchange activityRat liverLiver disordersPrimary siteForskolin administrationMicroM forskolinDextran 40Duct segmentsDibutyryl cAMPUseful preparationLiverSecretionForskolinImaging techniquesDuctLuminal spaceSyndrome
1993
Intracellular alkalinization stimulates bile flow and vesicular-mediated exocytosis in IPRL
Bruck R, Benedetti A, Strazzabosco M, Boyer J. Intracellular alkalinization stimulates bile flow and vesicular-mediated exocytosis in IPRL. American Journal Of Physiology 1993, 265: g347-g353. PMID: 8368316, DOI: 10.1152/ajpgi.1993.265.2.g347.Peer-Reviewed Original ResearchMeSH Keywords4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAlkaliesAnimalsAnionsBariumBicarbonatesBileBiological TransportColchicineExocytosisHorseradish PeroxidaseHydrogen-Ion ConcentrationIn Vitro TechniquesIntracellular MembranesLiverMalePotassium ChannelsRatsRats, Sprague-DawleyConceptsBiliary HCO3- excretionBile flowHCO3- excretionIntracellular alkalinizationHCO3- exchangerAcute alkaline loadVesicle-mediated exocytosisBiliary excretionTransient increasePH recoveryAlkaline loadMembrane depolarizationRat liverChannel blocker BaCl2Intact liverLiverAlkalinizationMM NH4ClHCO3- concentrationDisulfonic acidDIDSRat hepatocytesColchicinePretreatmentExocytosis
1991
Cl(-)-HCO3- exchanger in isolated rat hepatocytes: role in regulation of intracellular pH
Benedetti A, Strazzabosco M, Corasanti J, Haddad P, Graf J, Boyer J. Cl(-)-HCO3- exchanger in isolated rat hepatocytes: role in regulation of intracellular pH. American Journal Of Physiology 1991, 261: g512-g522. PMID: 1887897, DOI: 10.1152/ajpgi.1991.261.3.g512.Peer-Reviewed Original ResearchMeSH Keywords4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAmmoniaAnimalsBariumBicarbonatesCarrier ProteinsCells, CulturedChloride-Bicarbonate AntiportersChloridesHydrogen-Ion ConcentrationIntracellular FluidKineticsLiverMaleRatsRats, Inbred StrainsConceptsAlkaline loadPH-sensitive dye BCECFMM H2DIDSPHi recoveryRegulation of intracellularRat hepatocyte monolayersAcid extrudersMM DIDSRat hepatocytesElectrophysiological techniquesDye BCECFHCO3- exchangerIsolated rat hepatocytesConstant pHoIntracellularMembrane potentialHepatocyte monolayersHepatocytesAcid loading
1990
Effects of osmotic stresses on isolated rat hepatocytes. I. Ionic mechanisms of cell volume regulation
Corasanti J, Gleeson D, Boyer J. Effects of osmotic stresses on isolated rat hepatocytes. I. Ionic mechanisms of cell volume regulation. American Journal Of Physiology 1990, 258: g290-g298. PMID: 2305895, DOI: 10.1152/ajpgi.1990.258.2.g290.Peer-Reviewed Original ResearchMeSH Keywords4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAmilorideAnimalsBariumBicarbonatesBumetanideCells, CulturedChloridesHypertonic SolutionsHypotonic SolutionsKineticsLiverMaleOsmolar ConcentrationPotassium ChlorideQuinineRatsRats, Inbred StrainsSodium ChlorideEffects of osmotic stresses on isolated rat hepatocytes. II. Modulation of intracellular pH
Gleeson D, Corasanti J, Boyer J. Effects of osmotic stresses on isolated rat hepatocytes. II. Modulation of intracellular pH. American Journal Of Physiology 1990, 258: g299-g307. PMID: 2305896, DOI: 10.1152/ajpgi.1990.258.2.g299.Peer-Reviewed Original ResearchMeSH Keywords4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAnimalsBicarbonatesBuffersCells, CulturedCholineHydrogen-Ion ConcentrationHypertonic SolutionsHypotonic SolutionsIntracellular FluidKineticsLiverMaleOsmolar ConcentrationRatsRats, Inbred StrainsSodiumConceptsRegulatory volume decreaseAbsence of HCO3Presence of HCO3Acid-base transport systemsModulation of intracellularRat hepatocytesVolume regulationAcute withdrawalMM amilorideGreater intracellularHCO3- exchangeAcid effluxIsotonic mediumIsolated rat hepatocytesSame maneuverCell volume regulationHepatocytesNet acid effluxAmilorideSubconfluent monolayersDisulfonic acidIntracellularVolume decreaseHypotonic mediumHypertonic medium
1987
Anion transport in basolateral (sinusoidal) liver plasma-membrane vesicles of the little skate (Raja erinacea)
Hugentobler G, Fricker G, Boyer J, Meier P. Anion transport in basolateral (sinusoidal) liver plasma-membrane vesicles of the little skate (Raja erinacea). Biochemical Journal 1987, 247: 589-595. PMID: 3426551, PMCID: PMC1148453, DOI: 10.1042/bj2470589.Peer-Reviewed Original ResearchMeSH Keywords4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAnimalsBiological TransportCarbonyl Cyanide m-Chlorophenyl HydrazoneCell MembraneCholic AcidCholic AcidsElectric FishHydrogen-Ion ConcentrationIn Vitro TechniquesLiverMaleProbenecidSkates, FishSulfatesConceptsPlasma membrane vesiclesBasolateral liver plasma membrane vesiclesSulfate uptakeLiver plasma membrane vesiclesProtonophore carbonyl cyanideSulfate transport systemVertebrate evolutionMammalian liver cellsPH-equilibrated conditionsTransmembrane transportPH gradientLittle skateRaja erinaceaUptake 5Carbonyl cyanideCI inhibitionAnion transport inhibitorAlkaline pH gradientVesiclesAnion transportSkate hepatocytesCertain organic anionsUptakeLiver cellsElasmobranchsProperties of the canalicular bile acid transport system in rat liver
Meier P, Meier-Abt A, Boyer J. Properties of the canalicular bile acid transport system in rat liver. Biochemical Journal 1987, 242: 465-469. PMID: 3593261, PMCID: PMC1147728, DOI: 10.1042/bj2420465.Peer-Reviewed Original ResearchMeSH Keywords4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAnimalsBile Acids and SaltsBiological TransportCell MembraneLiverMaleRatsRats, Inbred StrainsStructure-Activity RelationshipTaurocholic Acid