2023
Organic Anion Transporting Polypeptide (OATP) 1B3 is a Significant Transporter for Hepatic Uptake of Conjugated Bile Acids in Humans
Pan Q, Zhu G, Xu Z, Zhu J, Ouyang J, Tong Y, Zhao N, Zhang X, Cheng Y, Zhang L, Tan Y, Li J, Zhang C, Chen W, Cai S, Boyer J, Chai J. Organic Anion Transporting Polypeptide (OATP) 1B3 is a Significant Transporter for Hepatic Uptake of Conjugated Bile Acids in Humans. Cellular And Molecular Gastroenterology And Hepatology 2023, 16: 223-242. PMID: 37146714, PMCID: PMC10394288, DOI: 10.1016/j.jcmgh.2023.04.007.Peer-Reviewed Original ResearchConceptsBA uptake transportersBile duct ligationHepatic neutrophil infiltrationCholestatic liver injuryProinflammatory cytokine productionCholic acid dietAdaptive protective responseLiver-specific overexpressionWild-type miceConjugated bile acidsUptake transportersPrimary hepatocytesUDCA feedingNeutrophil infiltrationBDL miceLiver injuryCytokine productionBile flowDuct ligationOrganic anion transporting polypeptide (OATP) 1B3Conjugated BAsTransgenic miceHepatic uptakeBile acidsProtective response
2017
Mechanisms of bile acid mediated inflammation in the liver
Li M, Cai SY, Boyer JL. Mechanisms of bile acid mediated inflammation in the liver. Molecular Aspects Of Medicine 2017, 56: 45-53. PMID: 28606651, PMCID: PMC5662014, DOI: 10.1016/j.mam.2017.06.001.Peer-Reviewed Original ResearchConceptsLiver injuryBile acidsCholestatic animal modelsCauses of cholestasisCholestatic liver injuryInnate immune cellsBiliary injuryNeutrophil recruitmentBile flowImmune cellsEffective therapyInflammatory responseAnimal modelsMolecular mediatorsCholestasisInjuryNovel targetLiverElevated levelsPathological processesMolecular mechanismsHepatocytesInflammationPatientsPathogenesis
2013
Biosynthesis and trafficking of the bile salt export pump, BSEP: Therapeutic implications of BSEP mutations
Soroka CJ, Boyer JL. Biosynthesis and trafficking of the bile salt export pump, BSEP: Therapeutic implications of BSEP mutations. Molecular Aspects Of Medicine 2013, 37: 3-14. PMID: 23685087, PMCID: PMC3784619, DOI: 10.1016/j.mam.2013.05.001.Peer-Reviewed Original ResearchConceptsBenign recurrent intrahepatic cholestasis type 2Progressive familial intrahepatic cholestasis type 2Cell biological aspectsBile salt export pumpTranslational regulationCell biologyMembrane transportersPrimary transporterBile salt-dependent bile flowType 2Bile acidsRate-limiting stepExport pumpDrug-induced cholestasisBiological aspectsBiosynthesisTraffickingTransportersBSEP mutationsMutationsCholestatic diseaseIntrahepatic cholestasisBile flowBiliary systemEnterohepatic circulation
2010
Cholestasis: Genetic and Acquired
Boyer J. Cholestasis: Genetic and Acquired. Seminars In Liver Disease 2010, 30: 113-115. PMID: 20422493, DOI: 10.1055/s-0030-1253220.Peer-Reviewed Original ResearchConceptsBile salt export pumpCholestatic liver diseaseMultidrug resistance protein 3Cholestatic liver injuryLiver diseaseBile acidsBile salt transportersIssue of SeminarsLiver injuryCholestatic disordersBile flowSulfate conjugatesSodium taurocholate co-transporting polypeptideBile duct-cannulated animalsBile formationExport pumpProtein 3Bile acid-independent bile flowSalt transportersTaurocholate co-transporting polypeptideOrganic solute transporters alphaDependent bile salt transporterApical sodium-dependent bile salt transporterFamilial intrahepatic cholestasis-1Drug-induced cholestasis
1999
Vasoactive intestinal polypeptide is a potent regulator of bile secretion from rat cholangiocytes
Cho W, Boyer J. Vasoactive intestinal polypeptide is a potent regulator of bile secretion from rat cholangiocytes. Gastroenterology 1999, 117: 420-428. PMID: 10419925, DOI: 10.1053/gast.1999.0029900420.Peer-Reviewed Original ResearchConceptsBile duct unitsEffect of VIPBile flowBile secretionDuct unitsIntestinal polypeptideFluid secretionBile duct-ligated ratsBile salt outputVasoactive intestinal polypeptideHepatocyte coupletsDose-dependent increaseDuct-ligated ratsBile pHVIP antagonistSite of actionSubstance PSecretory responseVIP responsesReceptor inhibitorsCAMP-independent pathwayBicarbonate secretionPotent stimulantSalt outputAbstractText
1998
Nitric oxide and guanosine 3′,5′‐cyclic monophosphate stimulate bile secretion in isolated rat hepatocyte couplets, but not in isolated bile duct units
Trauner M, Mennone A, Gigliozzi A, Fraioli F, Boyer J. Nitric oxide and guanosine 3′,5′‐cyclic monophosphate stimulate bile secretion in isolated rat hepatocyte couplets, but not in isolated bile duct units. Hepatology 1998, 28: 1621-1628. PMID: 9828227, DOI: 10.1002/hep.510280623.Peer-Reviewed Original ResearchConceptsDuctular bile secretionBile secretionBile duct unitsNitric oxideRat hepatocyte coupletsExchanger activityNO synthesisCl-/HCO3Bile acid-independent bile flowCell-permeant cGMP analogDuct unitsEndogenous NO synthesisHepatocyte coupletsEffect of NOCell-permeant cAMP analogsLevel of hepatocytesCyclic monophosphateHepatic NO synthesisRemoval of HCO3Canalicular bile secretionNG-monomethylBile flowCholeretic effectDuctular luminaL-arginineRole of glutathione in hepatic bile formation during reperfusion after cold ischemia of the rat liver
Koeppel T, Trauner M, Albert M, Arrese M, Rios-Velez L, Boyer J. Role of glutathione in hepatic bile formation during reperfusion after cold ischemia of the rat liver. Journal Of Hepatology 1998, 28: 812-819. PMID: 9625316, DOI: 10.1016/s0168-8278(98)80231-6.Peer-Reviewed Original ResearchConceptsBiliary glutathione excretionBile duct injuryCold ischemiaBile flowHepatic bile formationDuct injuryLiver transplantationBiliary tractBiliary excretionRat liverGlutathione excretionBasal bile flow ratesBile formationBile acid-independent bile flowOxidative stressLate reperfusion periodBile duct damageBile flow rateOnset of reperfusionIndependent bile flowBile duct morphologyBiliary glutathione concentrationsWistar rat liverLiver reperfusionDuct damage
1997
Enterohepatic circulation of scymnol sulfate in an elasmobranch, the little skate (Raja erinacea)
Fricker G, Wössner R, Drewe J, Fricker R, Boyer J. Enterohepatic circulation of scymnol sulfate in an elasmobranch, the little skate (Raja erinacea). American Journal Of Physiology 1997, 273: g1023-g1030. PMID: 9374698, DOI: 10.1152/ajpgi.1997.273.5.g1023.Peer-Reviewed Original ResearchConceptsEnterohepatic circulationScymnol sulfateBile alcohol sulfatesBile flowBiliary secretionCholeretic effectMajor bile saltsHepatic uptakeHepatic eliminationIntestinal epitheliumBileLiverSkate liverHepatic transportSpecific transport mechanismsBile alcoholsMajor metaboliteFirst direct demonstrationBile saltsCholyltaurinePerfusateLittle skatePolar metabolitesCompetitive mannerDirect demonstrationBombesin stimulates bicarbonate secretion from rat cholangiocytes: Implications for neural regulation of bile secretion
Cho W, Mennone A, Rydberg, Boyer J. Bombesin stimulates bicarbonate secretion from rat cholangiocytes: Implications for neural regulation of bile secretion. Gastroenterology 1997, 113: 311-321. PMID: 9207292, DOI: 10.1016/s0016-5085(97)70109-4.Peer-Reviewed Original ResearchConceptsBile secretionBicarbonate secretionRat liverBile duct-ligated ratsRole of bombesinHepatocyte coupletsEffect of bombesinDose-dependent increaseDuct-ligated ratsBile duct unitsCanalicular bile secretionBile pHSite of actionBile flowSubstance PReceptor inhibitorsNeural regulationAbstractTextBombesinBiliary transportDuct unitsSecretionLiverLuminal pHRat cholangiocytes
1993
Intracellular alkalinization stimulates bile flow and vesicular-mediated exocytosis in IPRL
Bruck R, Benedetti A, Strazzabosco M, Boyer J. Intracellular alkalinization stimulates bile flow and vesicular-mediated exocytosis in IPRL. American Journal Of Physiology 1993, 265: g347-g353. PMID: 8368316, DOI: 10.1152/ajpgi.1993.265.2.g347.Peer-Reviewed Original ResearchMeSH Keywords4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAlkaliesAnimalsAnionsBariumBicarbonatesBileBiological TransportColchicineExocytosisHorseradish PeroxidaseHydrogen-Ion ConcentrationIn Vitro TechniquesIntracellular MembranesLiverMalePotassium ChannelsRatsRats, Sprague-DawleyConceptsBiliary HCO3- excretionBile flowHCO3- excretionIntracellular alkalinizationHCO3- exchangerAcute alkaline loadVesicle-mediated exocytosisBiliary excretionTransient increasePH recoveryAlkaline loadMembrane depolarizationRat liverChannel blocker BaCl2Intact liverLiverAlkalinizationMM NH4ClHCO3- concentrationDisulfonic acidDIDSRat hepatocytesColchicinePretreatmentExocytosis
1992
Papaverine inhibits transcytotic vesicle transport and lipid excretion into bile in isolated perfused rat liver
Hayakawa T, Katagiri K, Hoshino M, Nakai T, Ohiwa T, Kumai T, Miyaji M, Takeuchi T, Corasanti J, Boyer J. Papaverine inhibits transcytotic vesicle transport and lipid excretion into bile in isolated perfused rat liver. Hepatology 1992, 16: 1036-1042. PMID: 1398483, DOI: 10.1002/hep.1840160429.Peer-Reviewed Original ResearchConceptsSodium taurocholate infusionBiliary excretionTaurocholate infusionPhosphodiesterase inhibitorBile acidsNonspecific smooth muscle relaxantSmooth muscle relaxantRat liver modelRat liverLipid excretionContinuous infusionBile flowMuscle relaxantsPapaverine inhibitsConstant infusionHorseradish peroxidaseInfusionPapaverineExcretionInhibitory effectCyclic GMPBileLiverLiver modelCyclic AMPRegulatory volume decrease stimulates bile flow, bile acid excretion, and exocytosis in isolated perfused rat liver
Bruck R, Haddad P, Graf J, Boyer J. Regulatory volume decrease stimulates bile flow, bile acid excretion, and exocytosis in isolated perfused rat liver. American Journal Of Physiology 1992, 262: g806-g812. PMID: 1590390, DOI: 10.1152/ajpgi.1992.262.5.g806.Peer-Reviewed Original ResearchEffects of Ca2+ agonists on cytosolic Ca2+ in isolated hepatocytes and on bile secretion in the isolated perfused rat liver
Nathanson M, Gautam A, Bruck R, Isales C, Boyer J. Effects of Ca2+ agonists on cytosolic Ca2+ in isolated hepatocytes and on bile secretion in the isolated perfused rat liver. Hepatology 1992, 15: 107-116. PMID: 1727785, DOI: 10.1002/hep.1840150119.Peer-Reviewed Original ResearchConceptsBile flowPerfusion pressureBile secretionCytosolic Ca2Rat liverProtein kinase CIonophore-induced increaseBromo-A23187Hepatic vasoconstrictionVasodilator papaverineProtein kinase C inhibitor HExtracellular Ca2Kinase CSecretionLiverVasopressinAgonist vasopressinBenzohydroquinoneEffect of Ca2HepatocytesActivate protein kinase CInhibitor HTwo-thirdsIsolated hepatocytesSensitive photoprotein aequorin
1990
DBcAMP stimulates vesicle transport and HRP excretion in isolated perfused rat liver
Hayakawa T, Bruck R, Ng O, Boyer J. DBcAMP stimulates vesicle transport and HRP excretion in isolated perfused rat liver. American Journal Of Physiology 1990, 259: g727-g735. PMID: 2173414, DOI: 10.1152/ajpgi.1990.259.5.g727.Peer-Reviewed Original ResearchConceptsBiliary excretionBile acidsBile acid excretionLiver 1Effects of adenosineRat liverElectron microscopic morphometric analysisTranscytotic vesicle pathwayLate peakHRP excretionHorseradish peroxidaseMin infusionBile flowExcretory functionAcid excretionO-dibutyryl cAMPPrimary hepatocyte culturesExcretionLiverDbcAMPCyclic monophosphateInfusionPericanalicular area
1989
Protein kinase C agonists inhibit bile secretion independently of effects on the microcirculation in the isolated perfused rat liver
Corasanti J, Smith N, Gordon E, Boyer J. Protein kinase C agonists inhibit bile secretion independently of effects on the microcirculation in the isolated perfused rat liver. Hepatology 1989, 10: 8-13. PMID: 2737605, DOI: 10.1002/hep.1840100103.Peer-Reviewed Original ResearchConceptsBile flowPerfusion pressureBile secretionVehicle dimethyl sulfoxideReversible decreaseConstant perfusate flowPhorbol dibutyrateVenous effluent concentrationsInactive phorbol ester 4Rat liver systemVascular redistributionHepatic microvasculaturePhorbol esterPhorbol ester 4Vasoactive agentsRole of hormonesPerfusate flowTrypan blue dyeAntagonist HProtein kinase C agonistsGm liverActive phorbol estersLiver systemRat liverOxygen consumptionDeterminants of glutathione efflux and biliary GSH/GSSG ratio in perfused rat liver
Ballatori N, Truong A, K. A, Boyer J. Determinants of glutathione efflux and biliary GSH/GSSG ratio in perfused rat liver. American Journal Of Physiology 1989, 256: g482-g490. PMID: 2564253, DOI: 10.1152/ajpgi.1989.256.3.g482.Peer-Reviewed Original ResearchConceptsGamma-glutamyl transferase activityO2 deliveryBiliary glutathioneHepatic effluxSinusoidal effluxKrebs-Ringer bicarbonate bufferGlutathione effluxRat liverEfflux of glutathioneGSH/GSSG ratioRed blood cellsPerfusion flow rateFluorocarbon emulsionsBile flowTissue GSHOxygen deliveryTransferase activityAT-125LiverBovine red blood cellsPerfusate compositionBlood cellsBileGSSG levelsGSSG ratio
1982
Hemodynamic effects on oxygen consumption and bile flow in isolated skate liver
Reed J, Smith N, Boyer J. Hemodynamic effects on oxygen consumption and bile flow in isolated skate liver. American Journal Of Physiology 1982, 242: g313-g318. PMID: 7065253, DOI: 10.1152/ajpgi.1982.242.4.g313.Peer-Reviewed Original ResearchConceptsPerfusion pressureBile flowSkate liverOxygen consumptionOrganic anion clearanceBile flow ratePortal vein pressurePerfusion of liversHemodynamic effectsVein pressureBile secretionFractional disappearance rateHepatic clearanceLiverDisappearance rateRinger's solutionPerfusionBileClearanceTaurocholateRaja erinaceaSulfobromophthaleinVivoMammalian speciesPerfusate
1977
Evaluation of a New Monkey Model for the Repeated Study of Bile Secretory Physiology
WOOD R, BAKER A, HALL A, BOYER J, MOOSSA A. Evaluation of a New Monkey Model for the Repeated Study of Bile Secretory Physiology. Annals Of Surgery 1977, 185: 349-355. PMID: 402891, PMCID: PMC1396621, DOI: 10.1097/00000658-197703000-00017.Peer-Reviewed Original ResearchConceptsBile flowSecretory physiologyMonkey modelBile salt secretion rateFinal infusion rateBile salt excretionBile salt outputHigher bile flowNew monkey modelSalt secretion rateBicarbonate outputPostoperative managementInfusion rateTaurocholate infusionSalt outputSalt excretionSecretion rateLong-term studiesRandom orderPeriod of monthsSecretinClearance
1973
Inhibition of Bilirubin Excretion in Man During Dehydrocholate Choleresis
Bloomer J, Boyer J, Klatskin G. Inhibition of Bilirubin Excretion in Man During Dehydrocholate Choleresis. Gastroenterology 1973, 65: 929-935. PMID: 4753351, DOI: 10.1016/s0016-5085(19)32986-5.Peer-Reviewed Original ResearchConceptsBilirubin excretionBilirubin concentrationExternal biliary drainagePlasma disappearance rateBiliary drainageBile flowCholeretic effectDilutional effectPatientsControl valuesExcretionSodium dehydrocholateControl periodDecholinDisappearance rateBileAdministrationMenCholeresisCumulative recovery
1970
Canalicular Bile flow and Bile Secretory Pressure Evidence for a non-bile salt dependent fraction in the isolated perfused rat liver
Boyer J, Klatskin G. Canalicular Bile flow and Bile Secretory Pressure Evidence for a non-bile salt dependent fraction in the isolated perfused rat liver. Gastroenterology 1970, 59: 853-859. PMID: 5486271, DOI: 10.1016/s0016-5085(19)33645-5.Peer-Reviewed Original Research