2012
Cross Talk Between Estradiol and mTOR Kinase in the Regulation of Ovarian Granulosa Proliferation
Yu J, Thomson T, Johnson J. Cross Talk Between Estradiol and mTOR Kinase in the Regulation of Ovarian Granulosa Proliferation. Reproductive Sciences 2012, 19: 143-151. PMID: 22383759, DOI: 10.1177/1933719111424447.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineCell ProliferationEstradiolFemaleGranulosa CellsRatsReceptors, EstradiolSignal TransductionTOR Serine-Threonine KinasesConceptsMammalian target of rapamycinGranulosa cellsFollicle growthAccelerated follicle growthReverse transcriptase polymerase chain reactionTranscriptase polymerase chain reactionOvarian granulosa cellsPresence of estradiolGene array analysisQuantitative reverse transcriptase polymerase chain reactionGrowth of ovarian folliclesPhosphorylation of mammalian target of rapamycinInhibin-alphaIntegrin beta 1Calcineurin expressionTarget of rapamycinPolymerase chain reactionMTOR inhibitorsEverolimusTemsirolimusBiphasic effectDrug treatmentOvarian folliclesReduced proliferationDose curves
2011
mTOR Controls Ovarian Follicle Growth by Regulating Granulosa Cell Proliferation
Yu J, Yaba A, Kasiman C, Thomson T, Johnson J. mTOR Controls Ovarian Follicle Growth by Regulating Granulosa Cell Proliferation. PLOS ONE 2011, 6: e21415. PMID: 21750711, PMCID: PMC3130037, DOI: 10.1371/journal.pone.0021415.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsApoptosisBlotting, WesternCarrier ProteinsCell DivisionCell Line, TransformedCell ProliferationDose-Response Relationship, DrugFemaleFlow CytometryG1 PhaseGranulosa CellsImmunosuppressive AgentsMaleMiceMice, Inbred C57BLOvarian FolliclePhosphorylationRatsRegulatory-Associated Protein of mTORRibosomal Protein S6 Kinases, 70-kDaSirolimusTOR Serine-Threonine KinasesConceptsAberrant mitotic figuresGranulosa cellsFollicle growthMitotic figuresMice treated with rapamycinFraction of granulosa cellsEffects of mTOR inhibitionRegulation of follicle growthDose-dependent increaseDose-dependent reductionRegulating granulosa cell proliferationGranulosa cell proliferationReduced compared to controlsMTOR pathway activationAberrant mitotic eventsExpression of RaptorOvarian follicle growthOvarian folliclesInhibition of mTORPresence of rapamycinMTOR inhibitionGranulosaInhibitor rapamycinConsequences of increased numbersMitotic index