2021
Interconnecting solvent quality, transcription, and chromosome folding in Escherichia coli
Xiang Y, Surovtsev IV, Chang Y, Govers SK, Parry BR, Liu J, Jacobs-Wagner C. Interconnecting solvent quality, transcription, and chromosome folding in Escherichia coli. Cell 2021, 184: 3626-3642.e14. PMID: 34186018, DOI: 10.1016/j.cell.2021.05.037.Peer-Reviewed Original Research
2016
DNA-relay mechanism is sufficient to explain ParA-dependent intracellular transport and patterning of single and multiple cargos
Surovtsev IV, Campos M, Jacobs-Wagner C. DNA-relay mechanism is sufficient to explain ParA-dependent intracellular transport and patterning of single and multiple cargos. Proceedings Of The National Academy Of Sciences Of The United States Of America 2016, 113: e7268-e7276. PMID: 27799522, PMCID: PMC5135302, DOI: 10.1073/pnas.1616118113.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateBiological TransportDNA PrimaseDNA, BacterialEscherichia coliEscherichia coli ProteinsProtein MultimerizationConceptsParA ATPase activityParA gradientCellular physiologyATP bindingBinds DNAIntracellular patterningChromosomal lociATP hydrolysisIntracellular transportInside cellsMultiple copiesMultiple cargoesDNA fluctuationsATPase activityIntracellular patternMacromolecular componentsCargoDNAPatterningMinimal systemBindingSignature patternsParBCargo dynamicsCells
2014
A Constant Size Extension Drives Bacterial Cell Size Homeostasis
Campos M, Surovtsev IV, Kato S, Paintdakhi A, Beltran B, Ebmeier SE, Jacobs-Wagner C. A Constant Size Extension Drives Bacterial Cell Size Homeostasis. Cell 2014, 159: 1433-1446. PMID: 25480302, PMCID: PMC4258233, DOI: 10.1016/j.cell.2014.11.022.Peer-Reviewed Original ResearchConceptsCell size homeostasisCell size controlSize homeostasisSingle-cell microscopyCell cycle controlCaulobacter crescentusAsymmetric divisionHomeostasis mechanismsBacteria Escherichia coliCell cycleEscherichia coliDifferent growth ratesCell growthCell lengthSize controlCell sizeHomeostasisBacteriaSize thresholdGrowth rateDivisionDirect experimental evidenceCrescentusSize paradigmColi
2013
The Bacterial Cytoplasm Has Glass-like Properties and Is Fluidized by Metabolic Activity
Parry BR, Surovtsev IV, Cabeen MT, O’Hern C, Dufresne ER, Jacobs-Wagner C. The Bacterial Cytoplasm Has Glass-like Properties and Is Fluidized by Metabolic Activity. Cell 2013, 156: 183-194. PMID: 24361104, PMCID: PMC3956598, DOI: 10.1016/j.cell.2013.11.028.Peer-Reviewed Original ResearchMeSH KeywordsBiophysical PhenomenaCaulobacter crescentusChromosomes, BacterialCytoplasmEscherichia coliPlasmids
2010
Spatial organization of the flow of genetic information in bacteria
Montero Llopis P, Jackson AF, Sliusarenko O, Surovtsev I, Heinritz J, Emonet T, Jacobs-Wagner C. Spatial organization of the flow of genetic information in bacteria. Nature 2010, 466: 77-81. PMID: 20562858, PMCID: PMC2896451, DOI: 10.1038/nature09152.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsCaulobacter crescentusChaperoninsChromosomes, BacterialDiffusionDNA, BacterialEndoribonucleasesEscherichia coliGene Expression Regulation, BacterialIn Situ Hybridization, FluorescenceLac OperonProtein BiosynthesisRibosomesRNA StabilityRNA TransportRNA, BacterialRNA, MessengerTranscription, GeneticConceptsSites of transcriptionC. crescentusCaulobacter crescentusEukaryotic cellsCellular physiologyMRNA decayMature mRNAMRNA processesRNase EMRNA substratesMRNA localizationGenetic informationGene expressionBacterial cellsEscherichia coliQuantitative fluorescenceCrescentusSitu hybridizationSpatial organizationMRNABacteriaLimited dispersionCellsTranscriptionTranslation
2007
Nickel-Dependent Oligomerization of the Alpha Subunit of Acetyl-Coenzyme A Synthase/Carbon Monoxide Dehydrogenase †
Tan X, Kagiampakis I, Surovtsev I, Demeler B, Lindahl P. Nickel-Dependent Oligomerization of the Alpha Subunit of Acetyl-Coenzyme A Synthase/Carbon Monoxide Dehydrogenase †. Biochemistry 2007, 46: 11606-11613. PMID: 17887777, PMCID: PMC2528952, DOI: 10.1021/bi7014663.Peer-Reviewed Original ResearchConceptsAlpha subunitCarbon monoxide dehydrogenaseSynthase/carbon monoxide dehydrogenaseApo-alphaHigh molecular weight speciesMolecular weight speciesRecombinant alpha subunitAcetyl-CoA synthase/carbon monoxide dehydrogenaseWeight speciesOpen conformationSulfur proteinA-clusterConformational changesSubunitsStructural scaffoldOligomerizationFe4S4 clusterSpeciesActive siteConformationDehydrogenaseInterrelated eventsActivationOligomerizesDimers
2000
Individual Escherichia coli cells studied from light scattering with the scanning flow cytometer
Shvalov A, Soini J, Surovtsev I, Kochneva G, Sivolobova G, Petrov A, Maltsev V. Individual Escherichia coli cells studied from light scattering with the scanning flow cytometer. Cytometry 2000, 41: 41-45. PMID: 10942895, DOI: 10.1002/1097-0320(20000901)41:1<41::aid-cyto6>3.0.co;2-n.Peer-Reviewed Original Research