2014
Prostate cancer bone metastases acquire resistance to androgen deprivation via WNT5A-mediated BMP-6 induction
Lee G, Kang D, Ha Y, Jung Y, Chung J, Min K, Kim T, Moon K, Chung J, Lee D, Kim W, Kim I. Prostate cancer bone metastases acquire resistance to androgen deprivation via WNT5A-mediated BMP-6 induction. British Journal Of Cancer 2014, 110: 1634-1644. PMID: 24518599, PMCID: PMC3960605, DOI: 10.1038/bjc.2014.23.Peer-Reviewed Original ResearchMeSH KeywordsAdultAndrogen AntagonistsAnilidesBone Morphogenetic Protein 6Bone NeoplasmsCell CommunicationCell Growth ProcessesCell Line, TumorHumansMaleMiddle AgedNeoplasm MetastasisNitrilesOrchiectomyProstatic Neoplasms, Castration-ResistantProto-Oncogene ProteinsReceptors, AndrogenRetrospective StudiesStromal CellsTosyl CompoundsWnt ProteinsWnt-5a ProteinConceptsCastration-resistant prostate cancerBone stromal cellsBone metastasesBone morphogenetic protein 6Castration resistanceCaP cell linesStromal cellsProstate cancerPrimary androgen deprivation therapyProstate-specific antigen progressionProstate cancer bone metastasisAndrogen-deprived mediumBone-tumor interactionAndrogen deprivation therapyFirst-line therapyCancer bone metastasisMetastatic prostate cancerAbsence of androgenAndrogen-depleted conditionsNF-κB pathwayCell linesPolymerase chain reaction arrayBMP-6 expressionAdvanced CaPDeprivation therapy
2013
Mechanism of pro‐tumorigenic effect of BMP‐6: Neovascularization involving tumor‐associated macrophages and IL‐1α
Kwon S, Lee G, Lee J, Iwakura Y, Kim W, Kim I. Mechanism of pro‐tumorigenic effect of BMP‐6: Neovascularization involving tumor‐associated macrophages and IL‐1α. The Prostate 2013, 74: 121-133. PMID: 24185914, DOI: 10.1002/pros.22734.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone Morphogenetic Protein 6CarcinogenesisCell DifferentiationCell Line, TumorCell ProliferationCoculture TechniquesEndothelium, VascularHumansInterleukin-1alphaMacrophagesMaleMiceMice, Inbred C57BLMice, KnockoutNeovascularization, PathologicNF-kappa BProstatic NeoplasmsSignal TransductionSmad1 ProteinConceptsBone morphogenetic protein 6Prostate cancer growthTumor-associated macrophagesIL-1APro-tumorigenic effectsCancer growthHuman prostate cancer cell linesHuman prostate cancer tissuesLNCaP human prostate cancer cell lineProstate cancer cell linesTube formationProstate cancer tissuesTHP-1 cellsEndothelial tube formationCancer cell linesIL-1αProstate cancerKnockout miceCD11b-DTRCancer tissuesTumor growthNF-kB1Endothelial cellsMacrophagesConditioned media
2011
Bone morphogenetic protein 6-induced interleukin-1β expression in macrophages requires PU.1/Smad1 interaction
Lee G, Jung Y, Lee J, Kim W, Kim I. Bone morphogenetic protein 6-induced interleukin-1β expression in macrophages requires PU.1/Smad1 interaction. Molecular Immunology 2011, 48: 1540-1547. PMID: 21571370, DOI: 10.1016/j.molimm.2011.04.019.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone Morphogenetic Protein 6Bone Morphogenetic Protein Receptors, Type IBone Morphogenetic Protein Receptors, Type IICells, CulturedExtracellular Signal-Regulated MAP KinasesFluorescent Antibody TechniqueImmunoblottingImmunoprecipitationInterleukin-1betaJNK Mitogen-Activated Protein KinasesMacrophagesMiceNitric Oxide Synthase Type IIProto-Oncogene ProteinsReverse Transcriptase Polymerase Chain ReactionSignal TransductionSmad1 ProteinTrans-ActivatorsConceptsBone morphogenetic protein 6Non-Smad pathwaysProtein 6Transcription factor PUSmads 1Physical interactionSmad1ExpressionMacrophages induce neuroendocrine differentiation of prostate cancer cells via BMP6‐IL6 Loop
Lee G, Kwon S, Lee J, Jeon S, Jang K, Choi H, Lee H, Kim W, Lee D, Kim I. Macrophages induce neuroendocrine differentiation of prostate cancer cells via BMP6‐IL6 Loop. The Prostate 2011, 71: 1525-1537. PMID: 21374653, DOI: 10.1002/pros.21369.Peer-Reviewed Original ResearchConceptsProstate cancer cellsHormone-refractory prostate cancerRefractory prostate cancerNeuroendocrine differentiationBone morphogenetic protein 6IL-6Cancer cellsProstate cancerInterleukin-6 knockout miceTRAMP-C2 cell lineHuman prostate cancer tissuesNeuroendocrine cellsCell linesParathyroid hormone-related peptideTRAMP-C2 prostate cancer cellsTRAMP-C2 cellsMacrophage-depleted miceProstate Cancer Neuroendocrine DifferentiationIL-6 expressionTHP-1 human monocytic cell lineHormone-related peptideProstate cancer tissuesHuman monocytic cell lineRAW 264.7 murine macrophage cell lineEffect of macrophages
2010
Induction of Interleukin-6 Expression by Bone Morphogenetic Protein-6 in Macrophages Requires Both SMAD and p38 Signaling Pathways*
Lee G, Kwon S, Lee J, Jeon S, Jang K, Choi H, Lee H, Kim W, Kim S, Kim I. Induction of Interleukin-6 Expression by Bone Morphogenetic Protein-6 in Macrophages Requires Both SMAD and p38 Signaling Pathways*. Journal Of Biological Chemistry 2010, 285: 39401-39408. PMID: 20889504, PMCID: PMC2998138, DOI: 10.1074/jbc.m110.103705.Peer-Reviewed Original ResearchConceptsBone morphogenetic protein 6BMP receptor type IIProtein 6Activin-like kinase 2Non-Smad pathwaysTranscription factor GATA4P38 Signaling PathwayTarget genesKnockdown experimentsKinase 2Signaling pathwaysSignaling mechanismReceptor type IISmadIntracellular levelsIL-6 inductionPathwayGrowth factorInductionExpressionInterleukin-6 expressionTranscriptionMacrophagesGenesGATA4
2009
Bone morphogenetic protein‐6 induces the expression of inducible nitric oxide synthase in macrophages
Kwon S, Lee G, Lee J, Kim W, Kim I. Bone morphogenetic protein‐6 induces the expression of inducible nitric oxide synthase in macrophages. Immunology 2009, 128: e758-e765. PMID: 19740337, PMCID: PMC2753926, DOI: 10.1111/j.1365-2567.2009.03079.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, NeutralizingBone Morphogenetic Protein 6Cells, CulturedCycloheximideDactinomycinHumansInterleukin-1betaMacrophages, PeritonealMiceNF-kappa BNitric Oxide Synthase Type IIProtein Synthesis InhibitorsRecombinant ProteinsSignal TransductionSmad ProteinsTumor Necrosis Factor-alphaConceptsBone morphogenetic proteinBMP-6Effects of BMPBone morphogenetic protein 6New protein synthesisMorphogenetic proteinsMurine macrophage cell lineMacrophage cell lineImportant regulatorProtein synthesisProtein 6Cell typesMouse peritoneal macrophagesCell linesActinomycin DNF-kappaBSmadExpressionDose-dependent mannerSynthaseNitric oxide synthasePathwayInductionInducible nitric oxide synthasePeritoneal macrophages