2019
The RNA exosome nuclease complex regulates human embryonic stem cell differentiation
Belair C, Sim S, Kim KY, Tanaka Y, Park IH, and, Wolin SL. The RNA exosome nuclease complex regulates human embryonic stem cell differentiation. Journal Of Cell Biology 2019, 218: 2564-2582. PMID: 31308215, PMCID: PMC6683745, DOI: 10.1083/jcb.201811148.Peer-Reviewed Original ResearchMeSH KeywordsCell DifferentiationCross-Linking ReagentsEndodermExosome Multienzyme Ribonuclease ComplexForkhead Transcription FactorsGene Expression RegulationHeLa CellsHuman Embryonic Stem CellsHumansLong Interspersed Nucleotide ElementsMesodermMicroRNAsPhenotypeRNARNA, Long NoncodingRNA, MessengerRNA, Small InterferingTranscription, GeneticTransgenesConceptsEmbryonic stem cellsESC differentiationTranscription networksSurveillance pathwayHuman embryonic stem cell differentiationGerm layersEmbryonic stem cell differentiationHuman embryonic stem cellsHuman ESC differentiationLINE-1 retrotransposonsStem cell differentiationTranscription factor crucialDevelopmental regulatorsMesendoderm formationDevelopmental genesRNA decayTranscription factorsSpecific miRNAsCell differentiationFactor crucialStem cellsPluripotencyExosomesDifferentiationRNA
2012
Excision of a Viral Reprogramming Cassette by Delivery of Synthetic Cre mRNA
Loh Y, Yang JC, De Los Angeles A, Guo C, Cherry A, Rossi DJ, Park I, Daley GQ. Excision of a Viral Reprogramming Cassette by Delivery of Synthetic Cre mRNA. Current Protocols In Stem Cell Biology 2012, 21: 4a.5.1-4a.5.16. PMID: 22605648, PMCID: PMC3397830, DOI: 10.1002/9780470151808.sc04a05s21.Peer-Reviewed Original ResearchConceptsPluripotent stem cellsTransgene-free methodsHuman iPS cellsResidual transgene expressionPatient-specific induced pluripotent stem cellsIPS cellsInduced pluripotent stem cellsStem cellsTransgene expressionFree humanHuman OCT4MRNA transfectionDrug screeningProtein transductionCre mRNADifferentiation potentialCell therapySingle vectorLow efficiencyCassetteRetroviral transfectionCre recombinaseEfficiencyTransfectionExperimental generationReprogramming human somatic cells into induced pluripotent stem cells (iPSCs) using retroviral vector with GFP.
Kim KY, Hysolli E, Park IH. Reprogramming human somatic cells into induced pluripotent stem cells (iPSCs) using retroviral vector with GFP. Journal Of Visualized Experiments 2012 PMID: 22491226, PMCID: PMC3466658, DOI: 10.3791/3804.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cellsInduced pluripotent stem cellsHuman somatic cellsHuman induced pluripotent stem cellsPluripotent stem cellsSomatic cellsIPSC coloniesStem cellsESC culture conditionsEmbryonic stem cellsPluripotency genesTranscription factorsRetroviral transgenesEctopic expressionGFP fluorescenceRetroviral vectorsHuman fibroblast cellsFibroblast cellsGFPCulture conditionsCellsAutologous cellsCellular sourceColoniesSurface markers
2009
A role for Lin28 in primordial germ-cell development and germ-cell malignancy
West JA, Viswanathan SR, Yabuuchi A, Cunniff K, Takeuchi A, Park IH, Sero JE, Zhu H, Perez-Atayde A, Frazier AL, Surani MA, Daley GQ. A role for Lin28 in primordial germ-cell development and germ-cell malignancy. Nature 2009, 460: 909-913. PMID: 19578360, PMCID: PMC2729657, DOI: 10.1038/nature08210.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCell LineChromosomal Proteins, Non-HistoneEmbryonic Stem CellsFemaleGene Expression Regulation, NeoplasticGerm CellsHumansMiceMice, Inbred C57BLNeoplasms, Germ Cell and EmbryonalPositive Regulatory Domain I-Binding Factor 1Repressor ProteinsRNA-Binding ProteinsTranscription FactorsTransgenes