2012
Two methods for full-length RNA sequencing for low quantities of cells and single cells
Pan X, Durrett RE, Zhu H, Tanaka Y, Li Y, Zi X, Marjani SL, Euskirchen G, Ma C, LaMotte RH, Park IH, Snyder MP, Mason CE, Weissman SM. Two methods for full-length RNA sequencing for low quantities of cells and single cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 110: 594-599. PMID: 23267071, PMCID: PMC3545756, DOI: 10.1073/pnas.1217322109.Peer-Reviewed Original ResearchConceptsRNA sequencingSingle cellsFull-length RNA sequencingSingle dorsal root ganglion neuronHigh-throughput sequencingGene expression patternsLower transcript levelsFull-length sequencesRandom oligonucleotide primersTranscript representationAbundant mRNAsK562 erythroleukemic cellsTranscript levelsMRNA sequencingExpression patternsBiological disciplinesErythroleukemic cellsExpression signaturesSequencingOligonucleotide primersFull lengthLow quantitiesDNA amplificationRNAPCR procedureAltered hematopoiesis in trisomy 21 as revealed through in vitro differentiation of isogenic human pluripotent cells
MacLean GA, Menne TF, Guo G, Sanchez DJ, Park IH, Daley GQ, Orkin SH. Altered hematopoiesis in trisomy 21 as revealed through in vitro differentiation of isogenic human pluripotent cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: 17567-17572. PMID: 23045682, PMCID: PMC3491455, DOI: 10.1073/pnas.1215468109.Peer-Reviewed Original ResearchMeSH KeywordsCell DifferentiationDown SyndromeGene Expression ProfilingHematopoiesisHumansKaryotypingPluripotent Stem CellsPolymerase Chain ReactionConceptsHuman embryonic stemPluripotent cellsHuman pluripotent cellsFetal liver stageEffects of trisomyPluripotent stem cellsDefinitive hematopoiesisIsogenic originColony-forming potentialColony-forming assaysΓ-globinEmbryonic stemIPS linesDifferentiation conditionsAltered hematopoiesisClonal variationHematopoietic cellsStem cellsHematopoiesisHematopoietic abnormalitiesChromosome 21Two- to fivefold increaseFetal liverTrisomic cellsMyeloid hematopoiesis
2010
Reprogramming of T Cells from Human Peripheral Blood
Loh YH, Hartung O, Li H, Guo C, Sahalie JM, Manos PD, Urbach A, Heffner GC, Grskovic M, Vigneault F, Lensch MW, Park IH, Agarwal S, Church GM, Collins JJ, Irion S, Daley GQ. Reprogramming of T Cells from Human Peripheral Blood. Cell Stem Cell 2010, 7: 15-19. PMID: 20621044, PMCID: PMC2913590, DOI: 10.1016/j.stem.2010.06.004.Peer-Reviewed Original Research