2012
Altered hematopoiesis in trisomy 21 as revealed through in vitro differentiation of isogenic human pluripotent cells
MacLean GA, Menne TF, Guo G, Sanchez DJ, Park IH, Daley GQ, Orkin SH. Altered hematopoiesis in trisomy 21 as revealed through in vitro differentiation of isogenic human pluripotent cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: 17567-17572. PMID: 23045682, PMCID: PMC3491455, DOI: 10.1073/pnas.1215468109.Peer-Reviewed Original ResearchConceptsHuman embryonic stemPluripotent cellsHuman pluripotent cellsFetal liver stageEffects of trisomyPluripotent stem cellsDefinitive hematopoiesisIsogenic originColony-forming potentialColony-forming assaysΓ-globinEmbryonic stemIPS linesDifferentiation conditionsAltered hematopoiesisClonal variationHematopoietic cellsStem cellsHematopoiesisHematopoietic abnormalitiesChromosome 21Two- to fivefold increaseFetal liverTrisomic cellsMyeloid hematopoiesis
2011
Altered Hematopoiesis in Trisomy 21 As Revealed Through In Vitro Differentiation of Isogenic Human Pluripotent Cells
MacLean G, Menne T, Park I, Daley G, Orkin S. Altered Hematopoiesis in Trisomy 21 As Revealed Through In Vitro Differentiation of Isogenic Human Pluripotent Cells. Blood 2011, 118: 921. DOI: 10.1182/blood.v118.21.921.921.Peer-Reviewed Original ResearchTrisomy 21Trisomic cellsFetal liverAdvisory CommitteeGATA1 mutationsPre-leukemic conditionAcute myeloid leukemiaTransient myeloproliferative disorderTrisomy 21 resultsCell linesDisomic controlsSomatic mutationsCFU-M coloniesColony forming assaysMultilineage hematopoietic progenitor cellsQuantitative RT-PCRHematopoietic progenitor cellsIL-6Myeloid leukemiaMouse modelMyeloproliferative disordersMegakaryoblastic leukemiaProgenitor populationsMyeloid progenitor populationsCD31/CD34