2009
A Robust Approach to Identifying Tissue-Specific Gene Expression Regulatory Variants Using Personalized Human Induced Pluripotent Stem Cells
Lee JH, Park IH, Gao Y, Li JB, Li Z, Daley GQ, Zhang K, Church GM. A Robust Approach to Identifying Tissue-Specific Gene Expression Regulatory Variants Using Personalized Human Induced Pluripotent Stem Cells. PLOS Genetics 2009, 5: e1000718. PMID: 19911041, PMCID: PMC2766639, DOI: 10.1371/journal.pgen.1000718.Peer-Reviewed Original ResearchMeSH KeywordsAllelesCell DifferentiationCell LineCells, CulturedCluster AnalysisComputational BiologyDNA, ComplementaryFlow CytometryGene Expression RegulationHuman Genome ProjectHumansInduced Pluripotent Stem CellsNucleic Acid Amplification TechniquesOrgan SpecificityRegulatory Elements, TranscriptionalReproducibility of ResultsConceptsCis-regulatory variantsPluripotent stem cellsRegulatory polymorphismsGene expressionAllele-specific gene expressionIPS cellsPrimary fibroblastsCell typesAllele-specific expressionHigh-throughput sequencingStem cellsHuman iPS cellsSkin primary fibroblastsRegulatory variantsTissue-specific variantsX chromosomeDifferentiated derivativesExpression locusCell differentiationSpecific tissuesLymphoblastoid cellsDigital-RNAPadlock probesExpressionCells
2008
Generation of human-induced pluripotent stem cells
Park IH, Lerou PH, Zhao R, Huo H, Daley GQ. Generation of human-induced pluripotent stem cells. Nature Protocols 2008, 3: 1180-1186. PMID: 18600223, DOI: 10.1038/nprot.2008.92.Peer-Reviewed Original ResearchConceptsPluripotent stem cellsEmbryonic stem cellsIPS cellsStem cellsPatient-specific pluripotent stem cellsHuman somatic cellsInduced pluripotent stem cellsHuman iPS cellsHuman-induced pluripotent stem cellsHuman primary fibroblastsTissue replacement therapiesSomatic cellsTranscription factorsPluripotent cellsEctopic expressionRetroviral transductionSource of cellsPrimary fibroblastsHuman fibroblast lineFibroblast linesPrimary human fibroblast linesCellsInvaluable toolTransplantation of cellsImmune rejection