2018
Genome-Wide Characterization of the Fur Regulatory Network Reveals a Link between Catechol Degradation and Bacillibactin Metabolism in Bacillus subtilis
Pi H, Helmann J. Genome-Wide Characterization of the Fur Regulatory Network Reveals a Link between Catechol Degradation and Bacillibactin Metabolism in Bacillus subtilis. MBio 2018, 9: 10.1128/mbio.01451-18. PMID: 30377275, PMCID: PMC6212828, DOI: 10.1128/mbio.01451-18.Peer-Reviewed Original ResearchConceptsRegulatory networksCatechol degradationPutative DNA binding sitesGenome-wide identificationGenome-wide characterizationFerric uptake regulatorIron-dependent transcriptional repressorCellular iron requirementsDNA binding sitesIron-sufficient conditionsIron-limiting conditionsHigh-affinity iron chelatorsEnvironmental aromatic compoundsSiderophore bacillibactinFur regulonGenomic viewApo-FurRepressor functionTranscriptional repressorRegulated genesChromatin immunoprecipitationPositive regulatorIron acquisitionNegative regulationGenetic analysis
2017
Sequential induction of Fur-regulated genes in response to iron limitation in Bacillus subtilis
Pi H, Helmann J. Sequential induction of Fur-regulated genes in response to iron limitation in Bacillus subtilis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2017, 114: 12785-12790. PMID: 29133393, PMCID: PMC5715773, DOI: 10.1073/pnas.1713008114.Peer-Reviewed Original ResearchConceptsFerric uptake regulatorHigh-affinity FeIron availabilityPerR regulonFur regulonSiderophore bacillibactinGenetic toolsChIP experimentsIron limitationCell transitionBacillus subtilisHigh-affinity bindingIron homeostasisBacterial cellsIntracellular ironSequential inductionRegulonBacillibactinEnzyme accessVivo occupancyGenesFurIron poolIron sufficiencyEfflux transporters
2012
A Putative P-Type ATPase Required for Virulence and Resistance to Haem Toxicity in Listeria monocytogenes
McLaughlin H, Xiao Q, Rea R, Pi H, Casey P, Darby T, Charbit A, Sleator R, Joyce S, Cowart R, Hill C, Klebba P, Gahan C. A Putative P-Type ATPase Required for Virulence and Resistance to Haem Toxicity in Listeria monocytogenes. PLOS ONE 2012, 7: e30928. PMID: 22363518, PMCID: PMC3283593, DOI: 10.1371/journal.pone.0030928.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphatasesAnimalsBacterial ProteinsBase SequenceBiological AssayComputational BiologyDrug Resistance, BacterialGene Expression Regulation, BacterialGenes, BacterialGenetic LociHemeHomeostasisIronListeria monocytogenesListeriosisMiceMolecular Sequence DataMothsMutagenesis, InsertionalMutationPlasmidsReal-Time Polymerase Chain ReactionRepressor ProteinsVirulenceConceptsPutative P-type ATPaseFerric uptake regulatorUptake of haemIntracellular pathogen Listeria monocytogenesWax moth Galleria mellonellaP-type ATPaseAcquisition of ironPathogen Listeria monocytogenesMoth Galleria mellonellaIron-deficient mediumMembrane proteinsMutantsHaem toxicityFree haemAlternative infection modelGalleria mellonellaIron homeostasisInhibition of growthFrvAVirulenceListeria monocytogenesHaemVirulence potentialFurDeficient medium