Featured Publications
Maturing Mycobacterium smegmatis peptidoglycan requires non-canonical crosslinks to maintain shape
Baranowski C, Welsh M, Sham L, Eskandarian H, Lim H, Kieser K, Wagner J, McKinney J, Fantner G, Ioerger T, Walker S, Bernhardt T, Rubin E, Rego E. Maturing Mycobacterium smegmatis peptidoglycan requires non-canonical crosslinks to maintain shape. ELife 2018, 7: e37516. PMID: 30324906, PMCID: PMC6231781, DOI: 10.7554/elife.37516.Peer-Reviewed Original ResearchConceptsPenicillin-binding proteinsAsymmetric polar growthRod-shaped bacteriaPolar growthPolar elongationShape maintenanceCell wallGenetic relationshipsDrug targetsUnusual crosslinksD-transpeptidasesSingle cellsPeptidoglycanCellsCrosslinksProteinMycobacteriaBacteriaPathogensTypes of crosslinksElongationGrowthMaintenanceTarget
2022
Mycobacterial serine/threonine phosphatase PstP is phosphoregulated and localized to mediate control of cell wall metabolism
Shamma F, Rego E, Boutte C. Mycobacterial serine/threonine phosphatase PstP is phosphoregulated and localized to mediate control of cell wall metabolism. Molecular Microbiology 2022, 118: 47-60. PMID: 35670057, PMCID: PMC10070032, DOI: 10.1111/mmi.14951.Peer-Reviewed Original ResearchConceptsCell wall metabolismWall metabolismCell wall-related proteinsSerine/threonine proteinCell wall regulationPhosphomimetic mutationReversible phosphorylationThreonine phosphataseEnvironmental stressRegulatory proteinsCell wallCorresponding mutationMycobacterial cell wallAntibiotic toleranceNovel substrateFhaAProteinPstPMycobacterium smegmatisWag31Certain substratesPeptidoglycanPhosphorylationRegulationMajor mechanismCell Wall Damage Reveals Spatial Flexibility in Peptidoglycan Synthesis and a Nonredundant Role for RodA in Mycobacteria
Melzer E, Kado T, García-Heredia A, Gupta K, Meniche X, Morita Y, Sassetti C, Rego E, Siegrist M. Cell Wall Damage Reveals Spatial Flexibility in Peptidoglycan Synthesis and a Nonredundant Role for RodA in Mycobacteria. Journal Of Bacteriology 2022, 204: e00540-21. PMID: 35543537, PMCID: PMC9210966, DOI: 10.1128/jb.00540-21.Peer-Reviewed Original ResearchConceptsPenicillin binding proteinsCell wall damagePeptidoglycan synthesisPeptidoglycan assemblyClass A Penicillin-Binding ProteinsNascent cell wallCell wall integrityCell wall assemblyCell-wall peptidoglycanCell wall synthesisRod-shaped bacteriaMycobacterium smegmatisPolar growthInternal turgorModel organismsMreB homologsNew inhibition strategyRod complexWall synthesisWall peptidoglycanWall integrityAnimal pathogensCell wallPathway functionSubcellular distribution
2020
The Conserved Translation Factor LepA Is Required for Optimal Synthesis of a Porin Family in Mycobacterium smegmatis
Fishbein S, Tomasi F, Wolf I, Dulberger C, Wang A, Keshishian H, Wallace L, Carr S, Ioerger T, Rego E, Rubin E. The Conserved Translation Factor LepA Is Required for Optimal Synthesis of a Porin Family in Mycobacterium smegmatis. Journal Of Bacteriology 2020, 203: 10.1128/jb.00604-20. PMID: 33361193, PMCID: PMC8095456, DOI: 10.1128/jb.00604-20.Peer-Reviewed Original ResearchMembrane-associated proteinsMembrane homeostasisCellular processesCell wallMembrane-associated proteomeWhole cell proteomeMajor membrane proteinOuter membrane porinsSteady-state abundanceDrug tolerance phenotypeMembrane proteomePorin familyTolerance phenotypeDeletion mutantsMembrane proteinsTranslation factorsMembrane porinsMajor porinsNutrient uptakeCell wall constituentsMycobacterial cell wallAntibiotic tolerancePorinProteomeDrug targets
2018
Characterization of Conserved and Novel Septal Factors in Mycobacterium smegmatis
Wu K, Zhang J, Baranowski C, Leung V, Rego E, Morita Y, Rubin E, Boutte C. Characterization of Conserved and Novel Septal Factors in Mycobacterium smegmatis. Journal Of Bacteriology 2018, 200: 10.1128/jb.00649-17. PMID: 29311277, PMCID: PMC5826036, DOI: 10.1128/jb.00649-17.Peer-Reviewed Original ResearchConceptsCell wallModel organismsCell wall regulationIntracellular membrane domainCell wall biosynthesisCell wall precursorsCell wall structureCritical regulatory functionsBacterial cell wallDifferent regulatory mechanismsWall biosynthesisDistant speciesMembrane domainsCoordinated regulationElongation functionStructural homologsWall precursorsDivision processRegulatory mechanismsHomologMycobacterial cell wallRegulatory functionsCell survivalDifferent regulatorsCell growth
2015
Phosphorylation of the Peptidoglycan Synthase PonA1 Governs the Rate of Polar Elongation in Mycobacteria
Kieser K, Boutte C, Kester J, Baer C, Barczak A, Meniche X, Chao M, Rego E, Sassetti C, Fortune S, Rubin E. Phosphorylation of the Peptidoglycan Synthase PonA1 Governs the Rate of Polar Elongation in Mycobacteria. PLOS Pathogens 2015, 11: e1005010. PMID: 26114871, PMCID: PMC4483258, DOI: 10.1371/journal.ppat.1005010.Peer-Reviewed Original ResearchConceptsPolar growthPenicillin binding proteinsCell elongationMost rod-shaped bacteriaNew cell wall materialTransglycosylase activityNormal cell lengthCell-wall peptidoglycanNew cell wallHuman pathogen Mycobacterium tuberculosisCell lengthRod-shaped bacteriaCell wall materialCell polesCell fitnessPolar elongationPathogen Mycobacterium tuberculosisWall peptidoglycanAntibiotic stressCytoplasmic regionCell wallBinding proteinPonA1Cell growthEnzymatic activity