2004
Delayed angiogenesis in aging rats and therapeutic effect of adenoviral gene transfer of VEGF.
Wang H, Keiser J, Olszewski B, Rosebury W, Robertson A, Kovesdi I, Gordon D. Delayed angiogenesis in aging rats and therapeutic effect of adenoviral gene transfer of VEGF. International Journal Of Molecular Medicine 2004, 13: 581-7. PMID: 15010860, DOI: 10.3892/ijmm.13.4.581.Peer-Reviewed Original ResearchAdenoviridaeAgingAnimalsBlotting, NorthernCell DivisionEndothelium, VascularEnzyme-Linked Immunosorbent AssayGene Transfer TechniquesHumansImmunoblottingImmunohistochemistryNeovascularization, PathologicNeovascularization, PhysiologicRatsTime FactorsTransfectionVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-2Impaired angiogenesis in SHR is associated with decreased KDR and MT1-MMP expression
Wang H, Olszewski B, Rosebury W, Wang D, Robertson A, Keiser J. Impaired angiogenesis in SHR is associated with decreased KDR and MT1-MMP expression. Biochemical And Biophysical Research Communications 2004, 315: 363-368. PMID: 14766216, DOI: 10.1016/j.bbrc.2004.01.059.Peer-Reviewed Original ResearchMeSH KeywordsAdenoviridaeAnimalsCell DivisionDNA, ComplementaryDown-RegulationEnzyme-Linked Immunosorbent AssayGene Transfer TechniquesHumansImmunoblottingImmunohistochemistryMatrix Metalloproteinases, Membrane-AssociatedMetalloendopeptidasesNeovascularization, PathologicProliferating Cell Nuclear AntigenRatsRats, Inbred SHRRats, Inbred WKYRats, Sprague-DawleySignal TransductionSpectrophotometryTime FactorsUp-RegulationVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-2ConceptsSpontaneous hypertensive ratsKinase insert domain-containing receptorMT1-MMP expressionAge-matched normotensive Wistar-KyotoImpaired angiogenesisEndothelial proliferationHypertension animal modelsNormotensive Wistar-KyotoSponge implantation modelLevel of angiogenesisSprague-Dawley ratsVEGF gene transferHypertensive ratsWistar KyotoDomain-containing receptorMembrane type 1 matrix metalloproteinaseAnimal modelsImmunohistological analysisMatrix metalloproteinaseImpaired VEGFSponge implantationAngiogenesisVEGF addition
2000
Rat sponge implant model: a new system for evaluating angiogenic gene transfer.
Wang H, Gordon D, Olszewski B, Song Y, Kovesdi I, Keiser J. Rat sponge implant model: a new system for evaluating angiogenic gene transfer. International Journal Of Molecular Medicine 2000, 6: 645-53. PMID: 11078823, DOI: 10.3892/ijmm.6.6.645.Peer-Reviewed Original ResearchMeSH KeywordsAdenoviridaeAnimalsBeta-GalactosidaseBlood VesselsDNA, RecombinantDose-Response Relationship, DrugEndothelial Growth FactorsGene Transfer TechniquesGene Transfer, HorizontalHumansImmunohistochemistryLymphokinesModels, CardiovascularNeovascularization, PathologicProstheses and ImplantsRatsRecombinant Fusion ProteinsSurgical SpongesTime FactorsVascular Endothelial Growth Factor AVascular Endothelial Growth FactorsVon Willebrand FactorConceptsBeta-galactosidase reporter geneSuch genesReporter geneGenesGene transferTransfection efficiencyAngiogenic genesGene therapyProtein levelsProtein expressionVEGF121 geneReplication-deficient adenovirus vectorModel systemSponge implant modelAngiogenic responseProtein injectionAngiogenesisAdenovirus vectorVEGF protein expressionAngiogenic effectsProtein concentrationEnhanced angiogenesisAngiogenic efficacyGene transfection efficiencyHigh levels
1998
Vascular Endothelial Growth Factor Upregulates the Expression of Matrix Metalloproteinases in Vascular Smooth Muscle Cells
Wang H, Keiser J. Vascular Endothelial Growth Factor Upregulates the Expression of Matrix Metalloproteinases in Vascular Smooth Muscle Cells. Circulation Research 1998, 83: 832-840. PMID: 9776730, DOI: 10.1161/01.res.83.8.832.Peer-Reviewed Original ResearchMeSH KeywordsAortaBiocompatible MaterialsCell DivisionCell MovementCells, CulturedCollagenCollagenasesDrug CombinationsEndothelial Growth FactorsEndothelium, VascularEnzyme ActivationEnzyme-Linked Immunosorbent AssayExtracellular MatrixGelsGene Expression Regulation, EnzymologicHumansLamininLymphokinesMatrix Metalloproteinase 1Matrix Metalloproteinase 9Muscle, Smooth, VascularNeovascularization, PhysiologicPhosphorylationProteoglycansProto-Oncogene ProteinsReceptor Protein-Tyrosine KinasesRNA, MessengerVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-1Vascular Endothelial Growth FactorsConceptsHuman smooth muscle cellsKinase insert domain-containing receptorSmooth muscle cellsVascular smooth muscle cellsSMC migrationSignal transduction pathwaysVascular endothelial growth factorExtracellular matrix barriersMuscle cellsNorthern blot analysisMajor cell typesEffects of VEGFGrowth factorDomain-containing receptorTransduction pathwaysInsert domain-containing receptorFlt-1Endothelial cells