2024
Targeting hypoxia and thrombospondin‐2 in diabetic wound healing
Huang Y, Xing H, Naud S, Kyriakides T. Targeting hypoxia and thrombospondin‐2 in diabetic wound healing. The FASEB Journal 2024, 38: e70091. PMID: 39383062, PMCID: PMC11486302, DOI: 10.1096/fj.202302429rrr.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acids, DicarboxylicAnimalsCell HypoxiaDiabetes Mellitus, ExperimentalFibroblastsHypoxiaHypoxia-Inducible Factor 1, alpha SubunitMaleMiceMice, Inbred C57BLMice, KnockoutThrombospondinsWound HealingConceptsThrombospondin-2Diabetic miceWound healingHIF-1aMatricellular protein thrombospondin-2Diabetic woundsImpaired wound healingWounds of diabetic miceDimethyloxalylglycine treatmentTargeting hypoxiaSustained hypoxiaDiabetic patientsTSP2 expressionCell dysfunctionIncreased neovascularizationDiabetic wound healingGenetic ablationDiabetic fibroblastsElevated glucoseReduced hypoxiaImprove healingImmunofluorescence analysisHIF-1a activationHypoxiaWestern blotting
2019
Elevated Thrombospondin 2 Contributes to Delayed Wound Healing in Diabetes
Kunkemoeller B, Bancroft T, Xing H, Morris AH, Luciano AK, Wu J, Fernandez-Hernando C, Kyriakides TR. Elevated Thrombospondin 2 Contributes to Delayed Wound Healing in Diabetes. Diabetes 2019, 68: 2016-2023. PMID: 31391172, PMCID: PMC6754242, DOI: 10.2337/db18-1001.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlood GlucoseDiabetes Mellitus, ExperimentalDiabetes Mellitus, Type 2FibroblastsHumansMiceMice, KnockoutNF-kappa BSignal TransductionSkinThrombospondinsWound HealingConceptsThrombospondin-2TSP2 expressionDiabetic control miceWound healingEffects of hyperglycemiaImpaired wound healingUnderlying pathological mechanismsDelayed Wound HealingMajor cellular sourceBlood vessel maturationGranulation tissue formationMajor complicationsDiabetic miceControl miceTreatment strategiesDiabetesPathological mechanismsDiabetic woundsAccelerated reepithelializationCellular sourceHigh glucoseHyperglycemiaMatricellular proteinExpression contributesHexosamine pathway
2018
Tunable Hydrogels Derived from Genetically Engineered Extracellular Matrix Accelerate Diabetic Wound Healing
Morris AH, Lee H, Xing H, Stamer DK, Tan M, Kyriakides TR. Tunable Hydrogels Derived from Genetically Engineered Extracellular Matrix Accelerate Diabetic Wound Healing. ACS Applied Materials & Interfaces 2018, 10: 41892-41901. PMID: 30424595, PMCID: PMC9996546, DOI: 10.1021/acsami.8b08920.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDiabetes Mellitus, ExperimentalExtracellular MatrixHydrogelsMiceMice, KnockoutNIH 3T3 CellsProtein EngineeringThrombospondinsWound HealingDecellularized materials derived from TSP2-KO mice promote enhanced neovascularization and integration in diabetic wounds
Morris AH, Stamer DK, Kunkemoeller B, Chang J, Xing H, Kyriakides TR. Decellularized materials derived from TSP2-KO mice promote enhanced neovascularization and integration in diabetic wounds. Biomaterials 2018, 169: 61-71. PMID: 29631168, PMCID: PMC5933884, DOI: 10.1016/j.biomaterials.2018.03.049.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAcellular DermisAnimalsDiabetes Mellitus, ExperimentalGene Knockdown TechniquesMiceNeovascularization, PathologicThrombospondinsTissue EngineeringTissue ScaffoldsWound HealingConceptsUltimate tensile strengthECM-based materialsTensile strengthMechanical testingDecellularized materialsElastic modulusSynthetic biomaterialsIntact slabsMatrix propertiesExtracellular matrix propertiesCell-derived ECMBiologic scaffoldsElectron microscopyEngineering controlsMaterialsWtEfficient integrationEnhanced vascularizationModulusPromigratory propertiesScaffoldsPropertiesBiomaterialsEnhanced remodelingGreater cell migration