2014
Generation of transgenic Hydra by embryo microinjection.
Juliano CE, Lin H, Steele RE. Generation of transgenic Hydra by embryo microinjection. Journal Of Visualized Experiments 2014, 51888. PMID: 25285460, PMCID: PMC4828061, DOI: 10.3791/51888.Peer-Reviewed Original ResearchConceptsTransgenic HydraFundamental biological processesEarly stage embryosPatches of tissuePure cell populationsMulticellular animalsStudy of regenerationGene functionMolecular functionsPhylum CnidariaParticular lineageTransgenic linesGene perturbationsSister groupTransgenic tissuesTransgenic methodsPromoter functionAsexual propagationBiological processesEmbryo microinjectionStage embryosLive cellsStem cellsHatchlingsBiochemical analysis
2012
High‐Efficiency Transfection and siRNA‐Mediated Gene Knockdown in Human Pluripotent Stem Cells
Ma Y, Lin H, Qiu C. High‐Efficiency Transfection and siRNA‐Mediated Gene Knockdown in Human Pluripotent Stem Cells. Current Protocols In Stem Cell Biology 2012, 21: 5c.2.1-5c.2.9. PMID: 22605647, DOI: 10.1002/9780470151808.sc05c02s21.Peer-Reviewed Original ResearchConceptsHigh transfection efficiencyPluripotent stem cellsTransfection efficiencyTransfection reagent Lipofectamine 2000Human embryonic stem cellsStem cellsHuman pluripotent stem cellsEmbryonic stem cellsPluripotent cell linesLipofectamine 2000SiRNA-mediated gene knockdownPluripotent cellsExpensive equipmentPluripotent genesGene knockdownProtocolEfficiencyPrimary cellsGenesCell linesCarriersCellsApplicationsTransfection