2011
Pinpointing the expression of piRNAs and function of the PIWI protein subfamily during spermatogenesis in the mouse
Beyret E, Lin H. Pinpointing the expression of piRNAs and function of the PIWI protein subfamily during spermatogenesis in the mouse. Developmental Biology 2011, 355: 215-226. PMID: 21539824, PMCID: PMC3443393, DOI: 10.1016/j.ydbio.2011.04.021.Peer-Reviewed Original ResearchConceptsPIWI proteinsXY bodyMouse testisAdult mouse testisEarly round spermatidsSex chromosomesGenomic sequencesPiRNAsChromatoid bodyCytological characterizationPachytene stageGerm cellsPhenotypic analysisAdult testisRound spermatidsProteinMeiosisSpermatogenesisCytoplasmDense bodiesSpermatocytesTestisPiRNAChromosomesTransposon
2009
Mili Interacts with Tudor Domain-Containing Protein 1 in Regulating Spermatogenesis
Wang J, Saxe JP, Tanaka T, Chuma S, Lin H. Mili Interacts with Tudor Domain-Containing Protein 1 in Regulating Spermatogenesis. Current Biology 2009, 19: 640-644. PMID: 19345100, PMCID: PMC2704239, DOI: 10.1016/j.cub.2009.02.061.Peer-Reviewed Original ResearchConceptsPIWI-interacting RNAsPiRNA biogenesisPIWI proteinsTudor domainChromatoid bodyMammalian Piwi proteinsMultiple Tudor domainsGermline-specific organelleTudor domain–containing protein-1Domain-containing protein 1RNA-independent interactionSmall noncoding RNAsProtein 1N-terminal regionGermline developmentGermline proteinsEpigenetic regulationNoncoding RNAsTerminal domainMILIRegulatory mechanismsMolecular mechanismsSpermatogenic defectsProteinBiogenesis
2008
MILI, a PIWI-interacting RNA-binding Protein, Is Required for Germ Line Stem Cell Self-renewal and Appears to Positively Regulate Translation*
Unhavaithaya Y, Hao Y, Beyret E, Yin H, Kuramochi-Miyagawa S, Nakano T, Lin H. MILI, a PIWI-interacting RNA-binding Protein, Is Required for Germ Line Stem Cell Self-renewal and Appears to Positively Regulate Translation*. Journal Of Biological Chemistry 2008, 284: 6507-6519. PMID: 19114715, PMCID: PMC2649106, DOI: 10.1074/jbc.m809104200.Peer-Reviewed Original ResearchConceptsGerm line stem cellsStem cellsArgonaute/PIWI protein familyMicro-RNA pathwayGerm line developmentMammalian stem cellsPIWI-interacting RNAsCap-binding complexRNA-binding proteinPIWI protein familyCellular mRNA levelsStem cell populationSelf-renewing divisionsArgonaute functionsPIWI proteinsRegulates TranslationPIWI subfamiliesEpigenetic regulationProtein familyCell maintenanceRNA interferenceChromatoid bodyMILIMolecular mechanismsEarly spermatocytes
2006
Interplay of PIWI/Argonaute protein MIWI and kinesin KIF17b in chromatoid bodies of male germ cells
Kotaja N, Lin H, Parvinen M, Sassone-Corsi P. Interplay of PIWI/Argonaute protein MIWI and kinesin KIF17b in chromatoid bodies of male germ cells. Journal Of Cell Science 2006, 119: 2819-2825. PMID: 16787948, DOI: 10.1242/jcs.03022.Peer-Reviewed Original ResearchConceptsChromatoid bodyGerm cellsPost-transcriptional regulationRound spermatidsTestis-specific memberMale germ cellsHaploid germ cellsArgonaute familyKinesin KIF17bRNA metabolismNucleocytoplasmic transportTranscriptional coactivatorKIF17bMIWIFunctional interplayNuclear envelopeGene expressionMolecular mechanismsEssential roleRNASpermatidsPotential mechanismsCellsCoactivatorNew light