Bioenergetics of serotonin transport by membrane vesicles derived from platelet dense granules.
Fishkes H, Rudnick G. Bioenergetics of serotonin transport by membrane vesicles derived from platelet dense granules. Journal Of Biological Chemistry 1982, 257: 5671-5677. PMID: 7068613, DOI: 10.1016/s0021-9258(19)83830-9.Peer-Reviewed Original ResearchConceptsPlatelet dense granulesMembrane vesiclesSerotonin transport activitySoluble enzymatic activityPresence of ATPCarbonyl cyanide p-trifluoromethoxyphenylhydrazoneAddition of ATPOsmotic lysatesTransport activityCyanide p-trifluoromethoxyphenylhydrazoneEnzymatic activityDensity gradient centrifugationVesiclesSerotonin transportVesicle interiorDense granulesMembrane potentialP-trifluoromethoxyphenylhydrazoneGradient centrifugationATPAssay mediumAdenine nucleotidesBioenergeticsNucleotidesGranules