2001
Synapsin I is expressed in epithelial cells: localization to a unique trans-Golgi compartment.
Bustos R, Kolen E, Braiterman L, Baines A, Gorelick F, Hubbard A. Synapsin I is expressed in epithelial cells: localization to a unique trans-Golgi compartment. Journal Of Cell Science 2001, 114: 3695-704. PMID: 11707521, DOI: 10.1242/jcs.114.20.3695.Peer-Reviewed Original ResearchConceptsSynapsin ITrans-Golgi compartmentSynaptic vesicle exocytosisProtein kinase ANon-neuronal cell linesBrain synapsin IEpithelial cellsNorthern blot analysisTrafficking pathwaysVesicle exocytosisVesicular compartmentsKinase AMyosin IIGolgi complexLimited proteolysisAnti-synapsin antibodiesPre-synaptic terminalsPeptide mapsBlot analysisCell linesCompartmentsCellsNeural tissueLiver cellsCytoskeleton
1995
Calmodulin-dependent protein kinases in rat glioblastoma.
Cheng EH, Gorelick FS, Czernik AJ, Bagaglio DM, Hait WN. Calmodulin-dependent protein kinases in rat glioblastoma. Molecular Cancer Research 1995, 6: 615-21. PMID: 7647041.Peer-Reviewed Original ResearchConceptsCaM-dependent protein kinaseProtein kinaseKinase II activitySignal transductionCaM kinase II activityKinase IICalmodulin-dependent protein kinaseCalcium-dependent signal transductionCaM-dependent protein kinase IIExogenous synapsin ICell cycle regulationII activityCaM kinase IIIElongation factor 2Protein kinase IICell linesCaM kinase IINumerous malignant cell linesSpecific peptide substratePhosphopeptide mappingKinase IIIAbnormal cell growthCycle regulationKinase activityKinase
1994
Antibodies to an epitope on synapsin I detect a protein associated with the endocytic compartment in non-neuronal cells.
Matovcik LM, Karapetian O, Czernik AJ, Marino CR, Kinder BK, Gorelick FS. Antibodies to an epitope on synapsin I detect a protein associated with the endocytic compartment in non-neuronal cells. European Journal Of Cell Biology 1994, 65: 327-40. PMID: 7536673.Peer-Reviewed Original ResearchConceptsClone 9 cellsEndocytic compartmentsPotential substrate proteinsDependent protein kinase IISynapsin ISmall intracellular vesiclesProtein kinase IIRat liver endosomesSubstrate proteinsPhosphorylation sequenceNon-neuronal cellsCLIP-170Intracellular vesiclesKinase IILarge endosomesPostnuclear supernatantEndosomesSensitive compartmentLiver endosomesProteinConfocal microscopyCell linesVesiclesIntestinal enterocytesCompartments
1992
Synaptic vesicle-associated Ca2+/calmodulin-dependent protein kinase II is a binding protein for synapsin I
Benfenati F, Valtorta F, Rubenstein J, Gorelick F, Greengard P, Czernik A. Synaptic vesicle-associated Ca2+/calmodulin-dependent protein kinase II is a binding protein for synapsin I. Nature 1992, 359: 417-420. PMID: 1328883, DOI: 10.1038/359417a0.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBrainCalcium-Calmodulin-Dependent Protein KinasesChromatography, High Pressure LiquidElectrophoresis, Gel, Two-DimensionalElectrophoresis, Polyacrylamide GelGene Expression Regulation, EnzymologicMolecular Sequence DataPhosphorylationProtein KinasesRatsReceptors, NeurotransmitterSubstrate SpecificitySynapsinsSynaptic VesiclesConceptsDependent protein kinase IIProtein kinase IIC-terminal regionKinase IISynapsin ISynaptic vesicle-associated phosphoproteinsAmino-terminal regionCarboxy-terminal regionKinase functionRegulatory domainProtein componentsMembrane phospholipidsProteinPhosphoproteinVesiclesEnzymeRegionBindingPhospholipidsDomainSynaptic
1990
Rabbit ileal villus cell brush border Na+/H+ exchange is regulated by Ca2+/calmodulin-dependent protein kinase II, a brush border membrane protein.
Cohen ME, Reinlib L, Watson AJ, Gorelick F, Rys-Sikora K, Tse M, Rood RP, Czernik AJ, Sharp GW, Donowitz M. Rabbit ileal villus cell brush border Na+/H+ exchange is regulated by Ca2+/calmodulin-dependent protein kinase II, a brush border membrane protein. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 8990-8994. PMID: 2174171, PMCID: PMC55086, DOI: 10.1073/pnas.87.22.8990.Peer-Reviewed Original ResearchConceptsDependent protein kinase IIProtein kinase IIKinase IIMembrane proteinsSynapsin IDependent protein kinase activityDependent protein kinaseBrush border membranePhosphorylation of sitesProtein kinase activityInhibitor peptideCaM kinase IIBrush border membrane proteinsBorder membraneSpecific inhibitor peptidePhosphopeptide mappingBrush borderProtein kinaseMicrovillus membrane proteinsKinase activityIleal brush-border membraneVillus cell brush border membraneApical membraneKinaseVesicle preparations
1989
Translocation of synapsin I in response to depolarization of isolated nerve terminals.
Sihra TS, Wang JK, Gorelick FS, Greengard P. Translocation of synapsin I in response to depolarization of isolated nerve terminals. Proceedings Of The National Academy Of Sciences Of The United States Of America 1989, 86: 8108-8112. PMID: 2510160, PMCID: PMC298224, DOI: 10.1073/pnas.86.20.8108.Peer-Reviewed Original ResearchConceptsSynapsin INerve terminalsRelease of neurotransmittersDepolarization of synaptosomesSmall synaptic vesiclesNeurotransmitter releaseSynaptic vesiclesDependent mannerIncubation mediumNerve terminal phosphoproteinDepolarizationPresent studyPhosphorylation stateCytosolic fractionPresence of Ca2TranslocationCa2Particulate fraction
1987
Calcium-calmodulin-stimulated protein kinase in developing pancreas
Gorelick FS, Chang A, Jamieson JD. Calcium-calmodulin-stimulated protein kinase in developing pancreas. American Journal Of Physiology 1987, 253: g469-g476. PMID: 3116856, DOI: 10.1152/ajpgi.1987.253.4.g469.Peer-Reviewed Original ResearchConceptsCalmodulin-dependent protein kinaseProtein kinase activityCDPK activityProtein kinaseKinase activityEndogenous proteinsType II calmodulin-dependent protein kinaseType II Ca2Adult pancreasCaM-binding proteinsRelative molecular weightEmbryonic pancreasPancreatic developmentPancreatic acinar cellsGel filtration chromatographySecretagogue responsivenessCalcium-calmodulinExogenous substratesKinaseSynapsin IPancreatic maturationProteinPancreatic supernatantPhosphorylationSecretory processPurification and properties of a multifunctional calcium/calmodulin-dependent protein kinase from rat pancreas
Cohn J, Kinder B, Jamieson J, Delahunt N, Gorelick F. Purification and properties of a multifunctional calcium/calmodulin-dependent protein kinase from rat pancreas. Biochimica Et Biophysica Acta 1987, 928: 320-331. PMID: 3105599, DOI: 10.1016/0167-4889(87)90192-3.Peer-Reviewed Original ResearchConceptsCalmodulin protein kinaseCalcium/calmodulin-dependent protein kinaseCalmodulin-dependent protein kinaseProtein kinaseMultifunctional calcium/calmodulin-dependent protein kinaseTwo-dimensional gel electrophoresisRibosomal proteinsThreonine residuesLarge subunitCellular functionsRibosomal substratesSubstrate specificityKinaseProteolytic degradationSubunitsGel electrophoresisHydrophobic chromatographyAffinity chromatographyPolyacrylamide gelsCalmodulinPurification procedureGel filtrationEnzyme preparationAutophosphorylationTissueCalcium-Calmodulin-Dependent Protein Kinase in Hyperplastic Human Parathyroid Glands
KINDER BK, DELAHUNT NG, JAMIESON JD, GORELICK FS. Calcium-Calmodulin-Dependent Protein Kinase in Hyperplastic Human Parathyroid Glands. Endocrinology 1987, 120: 170-177. PMID: 3096699, DOI: 10.1210/endo-120-1-170.Peer-Reviewed Original Research