2021
Role of SHH in Patterning Human Pluripotent Cells towards Ventral Forebrain Fates
Brady MV, Vaccarino FM. Role of SHH in Patterning Human Pluripotent Cells towards Ventral Forebrain Fates. Cells 2021, 10: 914. PMID: 33923415, PMCID: PMC8073580, DOI: 10.3390/cells10040914.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsMeSH KeywordsAnimalsBody PatterningHedgehog ProteinsHumansModels, BiologicalPluripotent Stem CellsProsencephalonSignal TransductionConceptsSonic hedgehogHuman neurodevelopmentHuman pluripotent cellsDiverse cellular compositionRole of SHHMaster regulatorPluripotent cellsCellular phenotypesVentral identityNeural organoidsExpression gradientsOrganoid systemsDisease modelingVitro systemPathway activationModel systemCellular compositionOrganoidsHuman developmentQuestions scientistsHedgehogBiologyCell-to-Cell Adhesion and Neurogenesis in Human Cortical Development: A Study Comparing 2D Monolayers with 3D Organoid Cultures
Scuderi S, Altobelli GG, Cimini V, Coppola G, Vaccarino FM. Cell-to-Cell Adhesion and Neurogenesis in Human Cortical Development: A Study Comparing 2D Monolayers with 3D Organoid Cultures. Stem Cell Reports 2021, 16: 264-280. PMID: 33513360, PMCID: PMC7878838, DOI: 10.1016/j.stemcr.2020.12.019.Peer-Reviewed Original Research
2016
Kv3.3 Channels Bind Hax-1 and Arp2/3 to Assemble a Stable Local Actin Network that Regulates Channel Gating
Zhang Y, Zhang XF, Fleming MR, Amiri A, El-Hassar L, Surguchev AA, Hyland C, Jenkins DP, Desai R, Brown MR, Gazula VR, Waters MF, Large CH, Horvath TL, Navaratnam D, Vaccarino FM, Forscher P, Kaczmarek LK. Kv3.3 Channels Bind Hax-1 and Arp2/3 to Assemble a Stable Local Actin Network that Regulates Channel Gating. Cell 2016, 165: 434-448. PMID: 26997484, PMCID: PMC4826296, DOI: 10.1016/j.cell.2016.02.009.Peer-Reviewed Original ResearchMeSH KeywordsActin CytoskeletonActin-Related Protein 2Actin-Related Protein 2-3 ComplexActin-Related Protein 3Adaptor Proteins, Signal TransducingAmino Acid SequenceCell MembraneMolecular Sequence DataMutationNeuronsPluripotent Stem CellsRac GTP-Binding ProteinsShaw Potassium ChannelsSignal TransductionSpinocerebellar AtaxiasConceptsCytoplasmic C-terminusProline-rich domainPlasma membraneHAX-1Actin nucleationC-terminusCortical actin filament networkLocal actin networkStem cell-derived neuronsActin filament networkCell-derived neuronsAnti-apoptotic proteinsActin cytoskeletonKv3.3 potassium channelActin assemblyActin structuresActin networkArp2/3Channel gatingFilament networkGrowth conesCerebellar neurodegenerationKv3.3TerminusPotassium channels
2014
Leptin signaling in astrocytes regulates hypothalamic neuronal circuits and feeding
Kim JG, Suyama S, Koch M, Jin S, Argente-Arizon P, Argente J, Liu ZW, Zimmer MR, Jeong JK, Szigeti-Buck K, Gao Y, Garcia-Caceres C, Yi CX, Salmaso N, Vaccarino FM, Chowen J, Diano S, Dietrich MO, Tschöp MH, Horvath TL. Leptin signaling in astrocytes regulates hypothalamic neuronal circuits and feeding. Nature Neuroscience 2014, 17: 908-910. PMID: 24880214, PMCID: PMC4113214, DOI: 10.1038/nn.3725.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAstrocytesCell CountEatingExcitatory Postsynaptic PotentialsGlial Fibrillary Acidic ProteinHypothalamusImmunohistochemistryIn Situ HybridizationLeptinMaleMelanocortinsMiceMice, KnockoutMicroscopy, ElectronNerve NetNeuronsPrimary Cell CulturePro-OpiomelanocortinPulmonary Gas ExchangeReal-Time Polymerase Chain ReactionRNA, MessengerSignal Transduction
2011
FGF Signaling Expands Embryonic Cortical Surface Area by Regulating Notch-Dependent Neurogenesis
Rash BG, Lim HD, Breunig JJ, Vaccarino FM. FGF Signaling Expands Embryonic Cortical Surface Area by Regulating Notch-Dependent Neurogenesis. Journal Of Neuroscience 2011, 31: 15604-15617. PMID: 22031906, PMCID: PMC3235689, DOI: 10.1523/jneurosci.4439-11.2011.Peer-Reviewed Original ResearchMeSH KeywordsAge FactorsAnalysis of VarianceAnimalsBrainBromodeoxyuridineCaspase 3Cell CountCell DifferentiationCells, CulturedCerebral CortexDNA-Binding ProteinsElectroporationEmbryo, MammalianEye ProteinsFatty Acid-Binding Protein 7Fatty Acid-Binding ProteinsFibroblast Growth FactorsGene Expression Regulation, DevelopmentalGreen Fluorescent ProteinsHomeodomain ProteinsKi-67 AntigenMiceMice, TransgenicMutationNerve Tissue ProteinsNeurogenesisNeuronsPaired Box Transcription FactorsPAX6 Transcription FactorReceptors, Fibroblast Growth FactorReceptors, NotchRepressor ProteinsSignal TransductionStem CellsT-Box Domain ProteinsTranscription FactorsConceptsCortical neurogenesisCortical surface area expansionCortical surface expansionCortical surface areaGrowth factor receptorEmbryonic day 12.5Fibroblast growth factor receptorFGFR mutantsNormal miceCortical layer structureCortical developmentNeurogenic stagesDominant negative FGFRLoss of functionRadial progenitorsNeurogenesisNotch pathway genesSevere deficitsFactor receptorDay 12.5Notch pathwayMiceSimultaneous activationGreater proportionFGFR activity
2009
Increased Brain Size in Autism—What It Will Take to Solve a Mystery
Vaccarino FM, Smith KM. Increased Brain Size in Autism—What It Will Take to Solve a Mystery. Biological Psychiatry 2009, 66: 313-315. PMID: 19643218, PMCID: PMC2803090, DOI: 10.1016/j.biopsych.2009.06.013.Commentaries, Editorials and LettersModeling premature brain injury and recovery
Scafidi J, Fagel DM, Ment LR, Vaccarino FM. Modeling premature brain injury and recovery. International Journal Of Developmental Neuroscience 2009, 27: 863-871. PMID: 19482072, PMCID: PMC2783901, DOI: 10.1016/j.ijdevneu.2009.05.009.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsBrain injuryAnimal modelsSignificant public health problemPremature brain injuryPublic health problemRelevant animal modelsNeurodevelopmental sequelaePreterm brainPreterm infantsPremature birthPreclinical modelsHealth problemsInjuryBrain structuresCellular mechanismsBrainInfantsSchool ageNeuroanatomical structuresVariable degreesRecent insightsEpigenetic mechanismsSequelaeTherapyRecovery
2007
Notch regulates cell fate and dendrite morphology of newborn neurons in the postnatal dentate gyrus
Breunig JJ, Silbereis J, Vaccarino FM, Šestan N, Rakic P. Notch regulates cell fate and dendrite morphology of newborn neurons in the postnatal dentate gyrus. Proceedings Of The National Academy Of Sciences Of The United States Of America 2007, 104: 20558-20563. PMID: 18077357, PMCID: PMC2154470, DOI: 10.1073/pnas.0710156104.Peer-Reviewed Original ResearchConceptsAbrogation of NotchCell cycle exitNeuronal fate determinationPrecursor cellsTransit-amplifying cellsFate determinationInducible gainCell fateCycle exitNeural precursor cellsModulates SurvivalMolecular controlPostnatal progenitor cellsLifelong additionHippocampal morphogenesisDifferentiation statePostnatal dentate gyrusNewborn granule cellsMembrane receptorsNeural stemGenetic ablationExpression of Notch1Structural plasticityProgenitor cellsEnvironmental modulatorsDeficiency in Inhibitory Cortical Interneurons Associates with Hyperactivity in Fibroblast Growth Factor Receptor 1 Mutant Mice
Smith K, Fagel DM, Stevens HE, Rabenstein RL, Maragnoli ME, Ohkubo Y, Picciotto MR, Schwartz ML, Vaccarino FM. Deficiency in Inhibitory Cortical Interneurons Associates with Hyperactivity in Fibroblast Growth Factor Receptor 1 Mutant Mice. Biological Psychiatry 2007, 63: 953-962. PMID: 17988653, DOI: 10.1016/j.biopsych.2007.09.020.Peer-Reviewed Original ResearchMeSH KeywordsAmphetamineAnimalsBehavior, AnimalBiogenic MonoaminesCell CountCentral Nervous System StimulantsCerebral CortexDisease Models, AnimalDopamine AgentsExploratory BehaviorFibroblast Growth Factor 1Glutamate DecarboxylaseHyperkinesisLocomotionMaleMethylphenidateMiceMice, KnockoutMotor ActivityNerve Tissue ProteinsNeural InhibitionNeuronsSignal TransductionConceptsInhibitory cortical circuitsCortical pyramidal neuronsD2 receptor antagonistGrowth factor receptor 1Spontaneous locomotor hyperactivityFibroblast growth factor receptor 1Factor receptor 1Inhibitory neuronal subtypesLocomotor hyperactivityDopamine agonistsCerebral cortexPyramidal neuronsBasal gangliaMotor hyperactivityReceptor antagonistInhibitory interneuronsTyrosine hydroxylaseCortical circuitsPsychiatric disordersLocomotor responseNeuronal subtypesReceptor 1Mutant miceDopamine transporterSpatial learning
2006
Midline radial glia translocation and corpus callosum formation require FGF signaling
Smith KM, Ohkubo Y, Maragnoli ME, Rašin M, Schwartz ML, Šestan N, Vaccarino FM. Midline radial glia translocation and corpus callosum formation require FGF signaling. Nature Neuroscience 2006, 9: 787-797. PMID: 16715082, DOI: 10.1038/nn1705.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAstrocytesCell MovementCell ShapeCerebral CortexCorpus CallosumDown-RegulationFemaleFibroblast Growth Factor 8Fibroblast Growth FactorsGrowth ConesMaleMiceMice, KnockoutMice, TransgenicNeurogliaReceptor, Fibroblast Growth Factor, Type 1Receptor, Fibroblast Growth Factor, Type 2RNA InterferenceSignal TransductionConceptsRadial glial cellsGlial cellsSomal translocationRadial gliaCorpus callosum formationReceptor 1 geneCallosal dysgenesisCerebral cortexFibroblast growth factor receptor 1 (FGFR1) geneIndusium griseumDorsomedial cortexDorsolateral cortexKnockout miceCortexAstrogliaApical endfeetFGFR1 geneAstrocytesGliaAxon guidanceDorsal midlinePrecise targetingCellsUnexpected roleFGF
2004
Chronic neonatal hypoxia leads to long term decreases in the volume and cell number of the rat cerebral cortex
Schwartz ML, Vaccarino F, Chacon M, Yan WL, Ment LR, Stewart WB. Chronic neonatal hypoxia leads to long term decreases in the volume and cell number of the rat cerebral cortex. Seminars In Perinatology 2004, 28: 379-388. PMID: 15693394, DOI: 10.1053/j.semperi.2004.10.009.Peer-Reviewed Original ResearchConceptsDays of hypoxiaPreterm birth resultsNeuronal sizeBirth resultsHypoxic exposureCell numberChronic neonatal hypoxiaChronic sublethal hypoxiaNeonatal rodent modelPerinatal period altersRat cerebral cortexNeuronal cell numberBcl-2Glial cell numbersNormoxic environmentPostnatal day 3Cortical cell numberSignificant neurodevelopmental disabilitiesWestern blot analysisPreterm birthNeonatal hypoxiaNormoxic exposureCerebral cortexChronic hypoxiaControl pupsLoss of Glutamatergic Pyramidal Neurons in Frontal and Temporal Cortex Resulting from Attenuation of FGFR1 Signaling Is Associated with Spontaneous Hyperactivity in Mice
Shin DM, Korada S, Raballo R, Shashikant CS, Simeone A, Taylor JR, Vaccarino F. Loss of Glutamatergic Pyramidal Neurons in Frontal and Temporal Cortex Resulting from Attenuation of FGFR1 Signaling Is Associated with Spontaneous Hyperactivity in Mice. Journal Of Neuroscience 2004, 24: 2247-2258. PMID: 14999075, PMCID: PMC6730438, DOI: 10.1523/jneurosci.5285-03.2004.Peer-Reviewed Original ResearchMeSH KeywordsAdrenergic alpha-AgonistsAmphetamineAnimalsCell DifferentiationCell DivisionFrontal LobeGlutamic AcidGuanfacineHumansHyperkinesisMiceMice, TransgenicNervous System MalformationsNeural InhibitionPyramidal CellsReceptor Protein-Tyrosine KinasesReceptor, Fibroblast Growth Factor, Type 1Receptors, Adrenergic, alpha-2Receptors, Fibroblast Growth FactorSignal TransductionStereotypic Movement DisorderTemporal LobeConceptsPyramidal neuronsCortical developmentTemporal areaSubcortical monoaminergic systemsGlutamatergic pyramidal neuronsCajal-Retzius cellsCortical GABAergic interneuronsCerebral cortical developmentAdrenergic receptor agonistEmbryonic neural progenitor cellsTemporal cortical areasReceptor gene productsNeural progenitor cellsEmbryonic brain developmentLocomotor hyperactivityRadial glia fibersCerebral cortexGlutamatergic neuronsBasal gangliaGABAergic interneuronsMonoaminergic systemsCortical plateReceptor agonistSpontaneous hyperactivityCortical areas
1999
6 Fibroblast Growth Factor Signaling Regulates Growth and Morphogenesis at Multiple Steps during Brain Development11This work represents a collaboration between the laboratories of the first two authors.
Vaccarino F, Schwartz M, Raballo R, Rhee J, Lyn-Cook R. 6 Fibroblast Growth Factor Signaling Regulates Growth and Morphogenesis at Multiple Steps during Brain Development11This work represents a collaboration between the laboratories of the first two authors. Current Topics In Developmental Biology 1999, 46: 179-200. PMID: 10417880, DOI: 10.1016/s0070-2153(08)60329-4.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsCentral nervous system regionsNervous system regionsCentral nervous systemRole of FGF2Growth factor familyCerebral cortexFibroblast growth factor (FGF) familyCortical developmentNervous systemFibroblast growth factor (FGF) signalingGrowth factor signalingSystem regionsFactor signalingMolecular mechanismsCoordinated activationDistinct patternsTarget genesFGF2FGFFactor familyCortex
1998
Development of the Cerebral Cortex: VII. Growth Factors: II
VACCARINO F, LOMBROSO P, Lombroso P. Development of the Cerebral Cortex: VII. Growth Factors: II. Journal Of The American Academy Of Child & Adolescent Psychiatry 1998, 37: 789-790. PMID: 9666636, DOI: 10.1097/00004583-199807000-00020.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements