2012
Learning and Memory Depend on Fibroblast Growth Factor Receptor 2 Functioning in Hippocampus
Stevens HE, Jiang GY, Schwartz ML, Vaccarino FM. Learning and Memory Depend on Fibroblast Growth Factor Receptor 2 Functioning in Hippocampus. Biological Psychiatry 2012, 71: 1090-1098. PMID: 22541947, PMCID: PMC3371339, DOI: 10.1016/j.biopsych.2012.03.013.Peer-Reviewed Original ResearchConceptsFGF receptor 2Fibroblast growth factorDentate gyrusReceptor 2Embryonic knockoutWater maze probe trialGrowth factor receptor 2Reference memoryFactor receptor 2Spatial reference memoryNeural stem cellsFibroblast growth factor receptor 2Immature neuronsCortical neuronsHippocampal volumeInducible knockout miceParvalbumin interneuronsShort-term learningGranule cellsKnockout miceSeparate cellular componentsHippocampusLong-term reference memoryAdult spatial memoryGrowth factorImpaired motor coordination and disrupted cerebellar architecture in Fgfr1 and Fgfr2 double knockout mice
Smith K, Williamson TL, Schwartz ML, Vaccarino FM. Impaired motor coordination and disrupted cerebellar architecture in Fgfr1 and Fgfr2 double knockout mice. Brain Research 2012, 1460: 12-24. PMID: 22578469, PMCID: PMC3361544, DOI: 10.1016/j.brainres.2012.04.002.Peer-Reviewed Original ResearchConceptsFibroblast growth factor receptorHuman GFAP promoterInner granule cell layerDKO miceGranule cell numberGranule cell progenitorsRadial glial stem cellsMidline glial structuresImpaired motor coordinationCerebellar sizeGranule cell layerDouble knockout miceGlial precursor cellsGlial stem cellsCell numberGranule neuron precursorsGrowth factor receptorGABA interneuronsGranule cell migrationCerebral cortexExternal granular layerMolecular layerMotor coordinationGranule cellsKnockout mice
2010
Astroglial cells in the external granular layer are precursors of cerebellar granule neurons in neonates
Silbereis J, Heintz T, Taylor MM, Ganat Y, Ment LR, Bordey A, Vaccarino F. Astroglial cells in the external granular layer are precursors of cerebellar granule neurons in neonates. Molecular And Cellular Neuroscience 2010, 44: 362-373. PMID: 20470892, PMCID: PMC2900521, DOI: 10.1016/j.mcn.2010.05.001.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornAstrocytesBasic Helix-Loop-Helix Transcription FactorsBeta-GalactosidaseCell LineageCerebellumGenes, ReporterGlial Fibrillary Acidic ProteinGreen Fluorescent ProteinsIntegrasesMiceMice, Inbred C57BLMice, TransgenicNeurogenesisNeuronsPromoter Regions, GeneticStem CellsTime FactorsConceptsExternal granule cell layerGranule cell precursorsInternal granule cell layerGranule cell layerGranule cellsRhombic lipAstroglial cellsProtein expression profilesGlial fibrillary acidic protein promoterCerebellar granule cell precursorsHuman glial fibrillary acidic protein promoterEmbryonic rhombic lipInducible Cre recombinaseNeuronal progenitor cellsReporter proteinFirst postnatal weekNeural stem cell markersLate embryogenesisCellular plasticityImmature granule cellsEarly postnatal developmentCell layerReporter geneCerebellar granule neuronsStem cell markers
2004
Fibroblast Growth Factor Receptor 1 Is Required for the Proliferation of Hippocampal Progenitor Cells and for Hippocampal Growth in Mouse
Ohkubo Y, Uchida AO, Shin D, Partanen J, Vaccarino FM. Fibroblast Growth Factor Receptor 1 Is Required for the Proliferation of Hippocampal Progenitor Cells and for Hippocampal Growth in Mouse. Journal Of Neuroscience 2004, 24: 6057-6069. PMID: 15240797, PMCID: PMC6729672, DOI: 10.1523/jneurosci.1140-04.2004.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, DifferentiationCell CountCell LineageCell ProliferationCells, CulturedHeredodegenerative Disorders, Nervous SystemHippocampusHumansIn Situ HybridizationLateral VentriclesMiceMice, TransgenicMutagenesis, Site-DirectedNeurogliaNeuronsPyramidal CellsReceptor Protein-Tyrosine KinasesReceptor, Fibroblast Growth Factor, Type 1Receptors, Fibroblast Growth FactorRNA, MessengerStem CellsTransgenesConceptsHippocampal ventricular zonesDentate gyrusGrowth factor receptor 1Fibroblast growth factor receptor 1Factor receptor 1Ventricular zoneNeural stem cellsPyramidal neuronsHippocampal growthProgenitor cellsGranule cellsReceptor 1Glial fibrillary acidic protein promoterHuman glial fibrillary acidic protein promoterEmbryonic dorsal telencephalonRadial glial-like cellsRadial glial progenitor cellsHippocampal dentate gyrusParvalbumin-containing interneuronsDG granule cellsHippocampal pyramidal neuronsStem cellsHippocampal progenitor cellsRole of FGFR1Glial progenitor cells
1987
Ganglioside inhibition of glutamate-mediated protein kinase C translocation in primary cultures of cerebellar neurons.
Vaccarino F, Guidotti A, Costa E. Ganglioside inhibition of glutamate-mediated protein kinase C translocation in primary cultures of cerebellar neurons. Proceedings Of The National Academy Of Sciences Of The United States Of America 1987, 84: 8707-8711. PMID: 2825205, PMCID: PMC299615, DOI: 10.1073/pnas.84.23.8707.Peer-Reviewed Original ResearchConceptsGranule cellsProtein kinase C translocationExcitatory amino acid neurotransmitter receptorsAmino acid neurotransmitter receptorsPrimary culturesGlutamate-mediated Ca2Cerebellar granule cellsHigh affinity recognition sitesPKC translocationNeurotransmitter receptorsRemoval of Ca2Cerebellar neuronsActivation of PKCC translocationNeuronal membranesGanglioside inhibitionRedistribution of PKCIncubation mediumGlutamateCa2PKCActivationCoexistence of GABA receptors and GABA-modulin in primary cultures of rat cerebellar granule cells
Vaccarino FM, Alho H, Santi MR, Guidotti A. Coexistence of GABA receptors and GABA-modulin in primary cultures of rat cerebellar granule cells. Journal Of Neuroscience 1987, 7: 65-76. PMID: 3027277, PMCID: PMC6568852, DOI: 10.1523/jneurosci.07-01-00065.1987.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarrier ProteinsCells, CulturedCerebellumFlunitrazepamFluorescent Antibody TechniqueGABA Plasma Membrane Transport ProteinsGamma-Aminobutyric AcidHistocytochemistryMembrane ProteinsMembrane Transport ProteinsMuscimolNerve Tissue ProteinsOrganic Anion TransportersRadioimmunoassayRatsReceptors, GABA-ASynaptosomesConceptsRat brain synaptosomesGranule cell culturesBrain synaptosomesGranule cellsGABA-modulinPrimary culturesCerebellar granule cell culturesRat cerebellar granule cellsPurkinje cell dendritesGranular cell layerGABA receptor complexGABA recognition siteCerebellar granule cellsGABAergic neuronsGABAergic interneuronsCell dendritesGlial cellsGABA receptorsIntact cell monolayersCell membrane preparationsCell culturesCerebellar interneuronsCell bodiesSpecific antibodiesSynaptosomes
1985
gamma-Aminobutyric acid- and benzodiazepine-induced modulation of [35S]- t-butylbicyclophosphorothionate binding to cerebellar granule cells
Gallo V, Wise BC, Vaccarino F, Guidotti A. gamma-Aminobutyric acid- and benzodiazepine-induced modulation of [35S]- t-butylbicyclophosphorothionate binding to cerebellar granule cells. Journal Of Neuroscience 1985, 5: 2432-2438. PMID: 4032005, PMCID: PMC6565307, DOI: 10.1523/jneurosci.05-09-02432.1985.Peer-Reviewed Original ResearchConceptsGamma-aminobutyric acidIntact cerebellar granule cellsCerebellar granule cellsGranule cellsT-butylbicyclophosphorothionateEffects of muscimolDose-dependent fashionAbsence of diazepamNeuroblastoma cell linesBenzodiazepine receptor ligandsSpecific bindingMicroM diazepamConvulsant activityCerebellar astrocytesMuscimolDiazepamLocke's solutionTotal radioactivityPrimary culturesReceptor ligandsCell linesReceptor complexCarboxylic acid methylesterInhibitionCells
1983
Loss of Purkinje cell‐associated benzodiazepine receptors spares a high affinity subpopulation: A study with pcd mutant mice
Vaccarino FM, Ghetti B, Wade SE, Rea MA, Aprison MH. Loss of Purkinje cell‐associated benzodiazepine receptors spares a high affinity subpopulation: A study with pcd mutant mice. Journal Of Neuroscience Research 1983, 9: 311-323. PMID: 6304330, DOI: 10.1002/jnr.490090308.Peer-Reviewed Original ResearchConceptsPopulation of receptorsBZ receptorsBenzodiazepine receptorsPurkinje cellsMutant miceHigh affinity subpopulationPcd miceGranule cell lossPurkinje cell degeneration mutant micePcd mutant miceNumber of receptorsDays of ageControl miceGlial cellsGranule cellsCell lossMicePcd mutantsReceptorsLow affinity sitesRegression analysisAgeDaysCellsSaturation data