2023
Pot1b −/− tumors activate G-quadruplex-induced DNA damage to promote telomere hyper-elongation
Takasugi T, Gu P, Liang F, Staco I, Chang S. Pot1b −/− tumors activate G-quadruplex-induced DNA damage to promote telomere hyper-elongation. Nucleic Acids Research 2023, 51: 9227-9247. PMID: 37560909, PMCID: PMC10516629, DOI: 10.1093/nar/gkad648.Peer-Reviewed Original ResearchConceptsDNA damage responseDamage responseReplication protein A (RPA) complexDependent DNA damage responseTelomere length homeostasisTelomere maintenance mechanismLength homeostasisTelomerase recruitmentPOT1 proteinsHuman POT1Mouse genomeLength maintenanceFunction disruptsReplicative immortalityTelomeresPOT1 mutationsDNA damageHuman cancersLonger telomeresPOT1bMaintenance mechanismsSerial transplantationA complexesSimilar mechanismMutations
2021
Fanci-FANCD2 Promotes Genome Stability and DNA Repair By Down-Regulating BLM Helicase Activity
Liang F, Nagarajan A, Pillai M, Sung P, Kupfer G. Fanci-FANCD2 Promotes Genome Stability and DNA Repair By Down-Regulating BLM Helicase Activity. Blood 2021, 138: 1113. DOI: 10.1182/blood-2021-152218.Peer-Reviewed Original ResearchDNA end resectionReplication forksGenome stabilityDNA repairResection activityRAD51 recruitmentEnd resectionAmino acid deletionBloom syndromeFanconi anemiaMutant cellsFA DNA repair pathwayAcid deletionDNA damageDNA damage hypersensitivityReplication fork collapseBLM helicase activityEfficient DNA repairHolliday junction dissolutionDNA damage repairDNA repair pathwaysRAD51 foci formationDouble Holliday junction dissolutionIsolation of proteinsHU sensitivity
2019
UAF1 DNA Binding Activity Is Critical for RAD51-Mediated Homologous DNA Pairing
Liang F, Miller A, Tang C, Sung P, Kupfer G. UAF1 DNA Binding Activity Is Critical for RAD51-Mediated Homologous DNA Pairing. Blood 2019, 134: 2497. DOI: 10.1182/blood-2019-130435.Peer-Reviewed Original ResearchHomologous DNA pairingHomologous recombinationDNA binding activityDNA repairDNA bindingDNA pairingHomologous DNAFanconi anemia DNA repair pathwayHR efficiencyFA core complexDNA damageDNA damage sensitivityDNA damage resistanceDNA-binding mutantSynaptic complex assemblyBinding activityE3 ligase activityDNA damage repairDNA repair pathwaysPresynaptic complex formationFANCD2 deubiquitinationGenome maintenanceComplex formationDeubiquitinase complexMutant proteinsBinding of FANCI-FANCD2 Complex to RNA and R-Loops Stimulates Robust FANCD2 Monoubiquitination
Liang Z, Liang F, Teng Y, Chen X, Liu J, Longerich S, Rao T, Green AM, Collins NB, Xiong Y, Lan L, Sung P, Kupfer GM. Binding of FANCI-FANCD2 Complex to RNA and R-Loops Stimulates Robust FANCD2 Monoubiquitination. Cell Reports 2019, 26: 564-572.e5. PMID: 30650351, PMCID: PMC6350941, DOI: 10.1016/j.celrep.2018.12.084.Peer-Reviewed Original ResearchConceptsR-loopsCo-transcriptional R-loopsFANCI-FANCD2 complexFanconi anemiaR-loop structuresSeries of RNADNA replicative stressGuanine-rich sequencesFANCD2 monoubiquitinationGenomic lociID2 complexReplicative stressFA cellsRNA transcriptionFA pathwayComplex bindsDNA hybridsCancer predispositionBone marrow failureDNA damageRNAMonoubiquitinationDevelopmental abnormalitiesMarrow failureSsRNA
2016
The Role of UAF1 in the Fanconi Anemia Pathway Regulation of Homologous Recombination-Mediated Genome Maintenance
Liang F, Longerich S, Tang C, Buzovestsky O, Xiong Y, Maranon D, Wiese C, Miller A, Sung P, Kupfer G. The Role of UAF1 in the Fanconi Anemia Pathway Regulation of Homologous Recombination-Mediated Genome Maintenance. Blood 2016, 128: 1041. DOI: 10.1182/blood.v128.22.1041.1041.Peer-Reviewed Original ResearchHomologous recombinationGenome maintenanceDNA damageFA DNA repair pathwayCancer-prone genetic diseasesFanconi anemiaDNA damage hypersensitivityFunctional synergySynaptic complex assemblyProtein complex formationRad51 presynaptic filamentD-loop reactionDNA repair functionDNA damage repairDNA binding activityCell linesFANCD2 ubiquitinationGenome stabilityComplex formationRAD51 recombinaseHuman cell linesBinds DNAPresynaptic filamentRecombinase RAD51UAF1