1995
A 55-kilodalton antigen encoded by a gene on a Borrelia burgdorferi 49-kilobase plasmid is recognized by antibodies in sera from patients with Lyme disease
Feng S, Das S, Lam T, Flavell RA, Fikrig E. A 55-kilodalton antigen encoded by a gene on a Borrelia burgdorferi 49-kilobase plasmid is recognized by antibodies in sera from patients with Lyme disease. Infection And Immunity 1995, 63: 3459-3466. PMID: 7642278, PMCID: PMC173477, DOI: 10.1128/iai.63.9.3459-3466.1995.Peer-Reviewed Original ResearchConceptsLate-stage Lyme diseaseEarly stage Lyme diseaseLyme diseaseIgM antibodiesSubset of patientsBorrelia burgdorferiLyme disease patientsEnzyme-linked immunosorbent assayRecombinant S1Disease patientsIgG antibodiesPulsed-field gel electrophoresisS1 antigenPatientsAntigenDiseaseSerum samplesImmunosorbent assaySerumAntibodiesB. burgdorferiRabbit antiBurgdorferiCultured spirochetesArthritis
1994
Outer surface proteins E and F of Borrelia burgdorferi, the agent of Lyme disease
Lam TT, Nguyen TP, Montgomery RR, Kantor FS, Fikrig E, Flavell RA. Outer surface proteins E and F of Borrelia burgdorferi, the agent of Lyme disease. Infection And Immunity 1994, 62: 290-298. PMID: 8262642, PMCID: PMC186099, DOI: 10.1128/iai.62.1.290-298.1994.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAntigens, BacterialBacterial Outer Membrane ProteinsBacterial ProteinsBase SequenceBlotting, WesternBorrelia burgdorferi GroupCloning, MolecularCodonFluorescent Antibody TechniqueGenes, BacterialHumansLipoproteinsLyme DiseaseMolecular Sequence DataMolecular WeightOperonRegulatory Sequences, Nucleic AcidRestriction MappingSequence AlignmentSequence Homology, Nucleic AcidSolubilityConceptsOspE genesMolecular massSignal peptidase IIConsensus cleavage sequenceTranscriptional unitsLeader sequenceCommon promoterBp downstreamOuter surface proteinsProtein EStop codonSurface lipoproteinsLabeling showBorrelia burgdorferiGenesHydrophobic domainCleavage sequenceSurface proteinsAmino acidsPeptidase IIProteinOuter surface protein EGel electrophoresisNucleotidesImmunofluorescence studies