2019
Borrelia miyamotoi infection leads to cross-reactive antibodies to the C6 peptide in mice and men
Koetsveld J, Platonov A, Kuleshov K, Wagemakers A, Hoornstra D, Ang W, Szekeres S, van Duijvendijk G, Fikrig E, Embers M, Sprong H, Hovius J. Borrelia miyamotoi infection leads to cross-reactive antibodies to the C6 peptide in mice and men. Clinical Microbiology And Infection 2019, 26: 513.e1-513.e6. PMID: 31404672, DOI: 10.1016/j.cmi.2019.07.026.Peer-Reviewed Original ResearchConceptsCross-reactive antibodiesC6 peptideEnzyme immunoassayLyme borreliosisBorrelia miyamotoi infectionBMD patientsExperimental murine infectionC6 enzyme immunoassayAvailable immunoblotNegative immunoblotMiyamotoi infectionTick bitePatient seraMurine infectionPatientsReactivity testingInfectionVlsE proteinBorrelia miyamotoiBorrelia burgdorferiMice
2018
Human Borrelia miyamotoi infection in California: Serodiagnosis is complicated by multiple endemic Borrelia species
Krause PJ, Carroll M, Fedorova N, Brancato J, Dumouchel C, Akosa F, Narasimhan S, Fikrig E, Lane RS. Human Borrelia miyamotoi infection in California: Serodiagnosis is complicated by multiple endemic Borrelia species. PLOS ONE 2018, 13: e0191725. PMID: 29420552, PMCID: PMC5805228, DOI: 10.1371/journal.pone.0191725.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, BacterialAntigensBorreliaBorrelia InfectionsCaliforniaEndemic DiseasesHumansSerologic TestsSpecies SpecificityConceptsBorrelia miyamotoi infectionMiyamotoi infectionB. miyamotoiB. miyamotoi diseaseB. miyamotoi infectionB. burgdorferi infectionHealth care professionalsWhole cell lysatesSeropositive subjectsSufficient serumClinical dataBurgdorferi infectionAntibody assaysSeropositive samplesCare professionalsBorrelia antigensInfectionSerumAntibodiesBorrelia burgdorferiAntigenI. pacificus ticksMiyamotoiTick infectionFever spirochetes
2017
Advances in Serodiagnostic Testing for Lyme Disease Are at Hand
Branda JA, Body BA, Boyle J, Branson BM, Dattwyler RJ, Fikrig E, Gerald NJ, Gomes-Solecki M, Kintrup M, Ledizet M, Levin AE, Lewinski M, Liotta LA, Marques A, Mead PS, Mongodin EF, Pillai S, Rao P, Robinson WH, Roth KM, Schriefer ME, Slezak T, Snyder J, Steere AC, Witkowski J, Wong SJ, Schutzer SE. Advances in Serodiagnostic Testing for Lyme Disease Are at Hand. Clinical Infectious Diseases 2017, 66: 1133-1139. PMID: 29228208, PMCID: PMC6019075, DOI: 10.1093/cid/cix943.Peer-Reviewed Original ResearchConceptsSerodiagnostic testingEnzyme immunoassayLyme diseaseIndirect fluorescence antibodySerologic assaysImmunoglobulin MEarly infectionFluorescence antibodyTesting protocolAlternative testing protocolsBorrelia burgdorferiB. burgdorferiWestern immunoblotAntigenDiseaseAntibodiesBurgdorferiLow sensitivitySynthetic peptidesAssays
2016
Variable Major Proteins as Targets for Specific Antibodies against Borrelia miyamotoi
Wagemakers A, Koetsveld J, Narasimhan S, Wickel M, Deponte K, Bleijlevens B, Jahfari S, Sprong H, Karan LS, Sarksyan DS, van der Poll T, Bockenstedt LK, Bins AD, Platonov AE, Fikrig E, Hovius JW. Variable Major Proteins as Targets for Specific Antibodies against Borrelia miyamotoi. The Journal Of Immunology 2016, 196: 4185-4195. PMID: 27076681, PMCID: PMC5008243, DOI: 10.4049/jimmunol.1600014.Peer-Reviewed Original ResearchConceptsVariable major proteinsB. miyamotoiSerologic testsC3H/HeN miceBorrelia miyamotoiHumoral immune responseInfected SCID miceTick-borne relapsing feverIgM reactivityHumoral immunityHeN miceIgM productionPassive transferSCID miceSerologic assaysCurrent serologic assaysImmune responseImmune serumRelapsing feverSpecific antibodiesProtein 1MiyamotoiIxodes ticksPatientsFever spirochetes
2014
Borrelia miyamotoi sensu lato Seroreactivity and Seroprevalence in the Northeastern United States - Volume 20, Number 7—July 2014 - Emerging Infectious Diseases journal - CDC
Krause PJ, Narasimhan S, Wormser GP, Barbour AG, Platonov AE, Brancato J, Lepore T, Dardick K, Mamula M, Rollend L, Steeves TK, Diuk-Wasser M, Usmani-Brown S, Williamson P, Sarksyan DS, Fikrig E, Fish D, . Borrelia miyamotoi sensu lato Seroreactivity and Seroprevalence in the Northeastern United States - Volume 20, Number 7—July 2014 - Emerging Infectious Diseases journal - CDC. Emerging Infectious Diseases 2014, 20: 1183-1190. PMID: 24960072, PMCID: PMC4073859, DOI: 10.3201/eid2007.131587.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, BacterialBorreliaBorrelia InfectionsFemaleHumansImmunoglobulin GLyme DiseaseMaleMiddle AgedNew EnglandSeroepidemiologic StudiesConceptsB. burgdorferiInfectious Diseases journal - CDCSeroprevalence of IgGB. burgdorferi antigensLyme disease endemic areaDisease-endemic areasAntibody testingHealthy personsLyme diseaseSerum samplesSeroprevalenceBurgdorferiInfectionSame tickUnited StatesSensu latoArea residentsPersonsBorrelia spSeroreactivityIgGAntigenDiseaseSerum
2013
SERUM ANTIBODIES TO BORRELIA BURGDORFERI, ANAPLASMA PHAGOCYTOPHILUM, AND BABESIA MICROTI IN RECAPTURED WHITE-FOOTED MICE
Magnarelli LA, Williams SC, Norris SJ, Fikrig E. SERUM ANTIBODIES TO BORRELIA BURGDORFERI, ANAPLASMA PHAGOCYTOPHILUM, AND BABESIA MICROTI IN RECAPTURED WHITE-FOOTED MICE. Journal Of Wildlife Diseases 2013, 49: 294-302. PMID: 23568904, PMCID: PMC3712766, DOI: 10.7589/2012-06-172.Peer-Reviewed Original ResearchConceptsB. microtiA. phagocytophilumB. burgdorferiBabesia microtiSolid-phase enzyme-linked immunosorbent assayAnaplasma phagocytophilumMouse immune systemEnzyme-linked immunosorbent assayIndirect fluorescent antibodyAntibody statusAntibody titersBorrelia burgdorferi sensu strictoSerum antibodiesTotal antibodyAntibody prevalenceNegative statusBurgdorferi sensu strictoImmune systemPatent infectionsEnd pointMiceImmunosorbent assayAntibodiesBorrelia burgdorferiFluorescent antibodyHuman Borrelia miyamotoi Infection in the United States
Krause PJ, Narasimhan S, Wormser GP, Rollend L, Fikrig E, Lepore T, Barbour A, Fish D. Human Borrelia miyamotoi Infection in the United States. New England Journal Of Medicine 2013, 368: 291-293. PMID: 23323920, PMCID: PMC3934646, DOI: 10.1056/nejmc1215469.Peer-Reviewed Original Research
2012
SERUM ANTIBODIES TO WHOLE-CELL AND RECOMBINANT ANTIGENS OF BORRELIA BURGDORFERI IN COTTONTAIL RABBITS
Magnarelli LA, Norris SJ, Fikrig E. SERUM ANTIBODIES TO WHOLE-CELL AND RECOMBINANT ANTIGENS OF BORRELIA BURGDORFERI IN COTTONTAIL RABBITS. Journal Of Wildlife Diseases 2012, 48: 12-20. PMID: 22247369, PMCID: PMC3261588, DOI: 10.7589/0090-3558-48.1.12.Peer-Reviewed Original Research
2010
SEASONAL PREVALENCE OF SERUM ANTIBODIES TO WHOLE CELL AND RECOMBINANT ANTIGENS OF BORRELIA BURGDORFERI AND ANAPLASMA PHAGOCYTOPHILUM IN WHITE-TAILED DEER IN CONNECTICUT
Magnarelli LA, Williams SC, Fikrig E. SEASONAL PREVALENCE OF SERUM ANTIBODIES TO WHOLE CELL AND RECOMBINANT ANTIGENS OF BORRELIA BURGDORFERI AND ANAPLASMA PHAGOCYTOPHILUM IN WHITE-TAILED DEER IN CONNECTICUT. Journal Of Wildlife Diseases 2010, 46: 781-790. PMID: 20688684, DOI: 10.7589/0090-3558-46.3.781.Peer-Reviewed Original ResearchMeSH KeywordsAnaplasma phagocytophilumAnimalsAnimals, WildAntibodies, BacterialBorrelia burgdorferiConnecticutDeerEhrlichiosisFemaleMaleSeasonsSeroepidemiologic StudiesConceptsRecombinant antigensInfected Ixodes scapularis ticksCoexistence of antibodiesAnaplasma phagocytophilumRespective causative agentsTick-borne infectionsEnzyme-linked immunosorbent assayIndirect fluorescent antibodyHuman granulocytic anaplasmosisPolyvalent enzyme-linked immunosorbent assaysIxodes scapularis ticksWhole blood samplesAntibody titersCurrent infectionWhole-cell B. burgdorferiBorrelia burgdorferi sensu strictoSerum antibodiesTotal antibodyBlood samplesHigher antibodyBurgdorferi sensu strictoSpecific recombinant antigensLyme borreliosisDeer seraImmunosorbent assay
2009
Antibodies against a Tick Protein, Salp15, Protect Mice from the Lyme Disease Agent
Dai J, Wang P, Adusumilli S, Booth CJ, Narasimhan S, Anguita J, Fikrig E. Antibodies against a Tick Protein, Salp15, Protect Mice from the Lyme Disease Agent. Cell Host & Microbe 2009, 6: 482-492. PMID: 19917502, PMCID: PMC2843562, DOI: 10.1016/j.chom.2009.10.006.Peer-Reviewed Original ResearchConceptsArthropod-borne pathogensTick-borne BorreliaTick salivary proteinsTick proteinsB. burgdorferiLyme diseaseDisease agentsTick-borne illnessB. burgdorferi infectionLyme disease agentHuman vaccinesSalp15Infection of miceB. burgdorferi antigensMicrobial toxinsMammalian hostsBorrelia burgdorferiPathogensMechanism of actionBurgdorferi infectionProtect miceMedical importanceBurgdorferiProtective capacityMice
2008
Preferential Protection of Borrelia burgdorferi Sensu Stricto by a Salp 15 Homologue in Ixodes ricinus Saliva
Hovius JW, Schuijt TJ, de Groot KA, Roelofs JJ, Oei GA, Marquart JA, de Beer R, Veer C, van der Poll T, Ramamoorthi N, Fikrig E, van Dam AP. Preferential Protection of Borrelia burgdorferi Sensu Stricto by a Salp 15 Homologue in Ixodes ricinus Saliva. The Journal Of Infectious Diseases 2008, 198: 1189-1197. PMID: 18752445, PMCID: PMC4317250, DOI: 10.1086/591917.Peer-Reviewed Original ResearchConceptsAntibody-mediated killingB. burgdorferiB. afzeliiB. gariniiEnzyme-linked immunosorbent assaySole causative agentBorrelia burgdorferi sensu latoBorrelia speciesSevere carditisOuter surface protein CImmune miceControl miceBorrelia burgdorferi sensu strictoBurgdorferi sensu latoSurface protein CSurvival advantageBurgdorferi sensu strictoSolid-phase overlayLyme borreliosisImmunosorbent assayProtein CCausative agentIxodes ticksBurgdorferiMice
2006
ANTIBODIES TO WHOLE-CELL OR RECOMBINANT ANTIGENS OF BORRELIA BURGDORFERI, ANAPLASMA PHAGOCYTOPHILUM, AND BABESIA MICROTI IN WHITE-FOOTED MICE
Magnarelli LA, Stafford KC, IJdo JW, Fikrig E. ANTIBODIES TO WHOLE-CELL OR RECOMBINANT ANTIGENS OF BORRELIA BURGDORFERI, ANAPLASMA PHAGOCYTOPHILUM, AND BABESIA MICROTI IN WHITE-FOOTED MICE. Journal Of Wildlife Diseases 2006, 42: 732-738. PMID: 17255439, DOI: 10.7589/0090-3558-42.4.732.Peer-Reviewed Original ResearchMeSH KeywordsAnaplasma phagocytophilumAnimalsAnimals, WildAntibodies, BacterialAntibodies, ProtozoanAntigens, BacterialAntigens, ProtozoanBabesia microtiBabesiosisBorrelia burgdorferiConnecticutEhrlichiosisEnzyme-Linked Immunosorbent AssayLyme DiseasePeromyscusRodent DiseasesSeroepidemiologic StudiesTick-Borne DiseasesConceptsEnzyme-linked immunosorbent assayB. burgdorferiOverall antibody prevalence rateBabesia microtiBorrelia burgdorferiAntibody prevalence ratesAnaplasma phagocytophilumA. phagocytophilumIndirect fluorescent antibodyWhite-footed miceWhole cell antigenTick-infested areasWhole-cell B. burgdorferiCurrent infectionPrevalence ratesMouse serumAntigenImmunosorbent assaySerum samplesRecombinant antigensAntibodiesSerumB. microtiMiceFluorescent antibody
2005
Seroprevalence of antibodies against Borrelia burgdorferi and Anaplasma phagocytophilum in cats.
Magnarelli LA, Bushmich SL, IJdo JW, Fikrig E. Seroprevalence of antibodies against Borrelia burgdorferi and Anaplasma phagocytophilum in cats. American Journal Of Veterinary Research 2005, 66: 1895-9. PMID: 16334946, DOI: 10.2460/ajvr.2005.66.1895.Peer-Reviewed Original ResearchConceptsRecombinant antigensSerum antibodiesB. burgdorferiHealthy catsBorrelia burgdorferiSeroprevalence of antibodiesWestern blot testingAnaplasma phagocytophilumA. phagocytophilumB. burgdorferi antigensWhole cell antigenA. phagocytophilum infectionIndirect fluorescent antibody stainingWestern blot analysisIxodes scapularis ticksSeropositivity rateWhole-cell B. burgdorferiSerum reactivityIll catsFluorescent antibody stainingClinical relevanceSera reactiveSpecific recombinant antigensMost catsOuter surface proteinsDetection of antibodies to Borrelia burgdorferi in naturally infected horses in the USA by enzyme-linked immunosorbent assay using whole-cell and recombinant antigens
Magnarelli L, Fikrig E. Detection of antibodies to Borrelia burgdorferi in naturally infected horses in the USA by enzyme-linked immunosorbent assay using whole-cell and recombinant antigens. Research In Veterinary Science 2005, 79: 99-103. PMID: 15924926, DOI: 10.1016/j.rvsc.2004.11.009.Peer-Reviewed Original Research
2004
TROSPA, an Ixodes scapularis Receptor for Borrelia burgdorferi
Pal U, Li X, Wang T, Montgomery RR, Ramamoorthi N, deSilva AM, Bao F, Yang X, Pypaert M, Pradhan D, Kantor FS, Telford S, Anderson JF, Fikrig E. TROSPA, an Ixodes scapularis Receptor for Borrelia burgdorferi. Cell 2004, 119: 457-468. PMID: 15537536, DOI: 10.1016/j.cell.2004.10.027.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibodies, BacterialAntigens, SurfaceBacterial Outer Membrane ProteinsBacterial VaccinesBase SequenceBorrelia burgdorferiCloning, MolecularGene Expression RegulationHost-Parasite InteractionsIntestinesIxodesLipoproteinsMiceMice, Inbred C3HMolecular Sequence DataReceptors, Cell SurfaceRecombinant ProteinsConceptsLyme disease agent Borrelia burgdorferiSurvival of spirochetesTick receptorMammalian hostsRNA interferenceEfficient colonizationTROSPAOuter surface protein ABorrelia burgdorferiPathogen adherencePathogen transmissionProtein AB. burgdorferi outer surface protein AMRNA levelsIxodes scapularisB. burgdorferiColonizationSurface protein AReceptorsArthropodsBurgdorferiMammalsRepressionSpirochetesTicksBorrelia burgdorferi Changes Its Surface Antigenic Expression in Response to Host Immune Responses
Liang FT, Yan J, Mbow ML, Sviat SL, Gilmore RD, Mamula M, Fikrig E. Borrelia burgdorferi Changes Its Surface Antigenic Expression in Response to Host Immune Responses. Infection And Immunity 2004, 72: 5759-5767. PMID: 15385475, PMCID: PMC517580, DOI: 10.1128/iai.72.10.5759-5767.2004.Peer-Reviewed Original ResearchConceptsSurface antigenic expressionAntigenic expressionImmune attackImmune responseSevere combined immunodeficiency miceHostile immune environmentVigorous immune responseB. burgdorferiCombined immunodeficiency miceHost immune responseOspC antibodiesOuter surface protein CImmune environmentDecorin-binding protein AImmunocompetent miceImmune conditionsImmunodeficiency miceSurface protein CImmune pressureVlsE expressionLyme disease spirocheteProtein CBorrelia burgdorferiOspC expressionMammalian infectionA comparison of serologic tests for the detection of serum antibodies to whole-cell and recombinant Borrelia burgdorferi antigens in cattle.
Magnarelli LA, Bushmich SL, Sherman BA, Fikrig E. A comparison of serologic tests for the detection of serum antibodies to whole-cell and recombinant Borrelia burgdorferi antigens in cattle. Canadian Veterinary Journal 2004, 45: 667-73. PMID: 15368740, PMCID: PMC546445.Peer-Reviewed Original ResearchMeSH KeywordsAnaplasma phagocytophilumAnimalsAntibodies, BacterialAntigens, BacterialBlotting, WesternBorrelia burgdorferiCattleCattle DiseasesConnecticutEnzyme-Linked Immunosorbent AssayFemaleFluorescent Antibody Technique, IndirectLyme DiseaseMaleMolecular WeightRecombinant ProteinsReproducibility of ResultsSensitivity and SpecificityTick InfestationsConceptsPolyvalent enzyme-linked immunosorbentVlsE antigenBorrelia burgdorferi antigensRecombinant antigensAntibody staining methodsHealthy dairyB. burgdorferi antigensIndirect fluorescent-antibody staining methodsCattle seraHigh specificityLeptospira interrogans serovarsBrucella spWhole cell antigenSerum samplesWestern blot procedureP39 antigenBorrelia burgdorferi outer surface protein (osp) B expression independent of ospA
Liang FT, Caimano MJ, Radolf JD, Fikrig E. Borrelia burgdorferi outer surface protein (osp) B expression independent of ospA. Microbial Pathogenesis 2004, 37: 35-40. PMID: 15194158, DOI: 10.1016/j.micpath.2004.02.007.Peer-Reviewed Original ResearchAnimalsAntibodies, BacterialAntigens, BacterialAntigens, SurfaceBacterial Outer Membrane ProteinsBacterial VaccinesBorrelia burgdorferiDisease Models, AnimalGene Expression Regulation, BacterialLipoproteinsLyme DiseaseMiceMice, Inbred BALB CMice, SCIDPromoter Regions, GeneticRNA, BacterialRNA, MessengerTicksTranscription, GeneticUSE OF RECOMBINANT ANTIGENS OF BORRELIA BURGDORFERI AND ANAPLASMA PHAGOCYTOPHILUM IN ENZYME-LINKED IMMUNOSORBENT ASSAYS TO DETECT ANTIBODIES IN WHITE-TAILED DEER
Magnarelli LA, IJdo JW, Ramakrishnan U, Henderson DW, Stafford KC, Fikrig E. USE OF RECOMBINANT ANTIGENS OF BORRELIA BURGDORFERI AND ANAPLASMA PHAGOCYTOPHILUM IN ENZYME-LINKED IMMUNOSORBENT ASSAYS TO DETECT ANTIBODIES IN WHITE-TAILED DEER. Journal Of Wildlife Diseases 2004, 40: 249-258. PMID: 15362824, DOI: 10.7589/0090-3558-40.2.249.Peer-Reviewed Original ResearchPreferential Presence of Decorin-Binding Protein B (BBA25) and BBA50 Antibodies in Cerebrospinal Fluid of Patients with Neurologic Lyme Disease
Fikrig E, Coyle PK, Schutzer SE, Chen M, Deng Z, Flavell RA. Preferential Presence of Decorin-Binding Protein B (BBA25) and BBA50 Antibodies in Cerebrospinal Fluid of Patients with Neurologic Lyme Disease. Journal Of Clinical Microbiology 2004, 42: 1243-1246. PMID: 15004083, PMCID: PMC356844, DOI: 10.1128/jcm.42.3.1243-1246.2004.Peer-Reviewed Original Research