2019
The role of Mannose Binding Lectin in the immune response against Borrelia burgdorferi sensu lato
Coumou J, Wagemakers A, Narasimhan S, Schuijt TJ, Ersoz JI, Oei A, de Boer OJ, Roelofs JJTH, Fikrig E, Hovius JW. The role of Mannose Binding Lectin in the immune response against Borrelia burgdorferi sensu lato. Scientific Reports 2019, 9: 1431. PMID: 30723261, PMCID: PMC6363739, DOI: 10.1038/s41598-018-37922-8.Peer-Reviewed Original ResearchConceptsMannose-Binding LectinB. burgdorferiImmune responseComplement systemRole of MBLMBL-deficient miceWhole blood stimulationIgG serum antibodiesB. burgdorferi infectionB. burgdorferi numbersHost complement systemMechanism warrants further investigationSerum-sensitive isolatesBorrelia burgdorferi sensu lato groupWarrants further investigationBorrelia burgdorferi sensu latoLater time pointsBinding lectinsSevere courseBlood stimulationDetectable antibodiesBurgdorferi sensu latoSerum antibodiesMBL deficiencyDeficient mice
2017
Advances in Serodiagnostic Testing for Lyme Disease Are at Hand
Branda JA, Body BA, Boyle J, Branson BM, Dattwyler RJ, Fikrig E, Gerald NJ, Gomes-Solecki M, Kintrup M, Ledizet M, Levin AE, Lewinski M, Liotta LA, Marques A, Mead PS, Mongodin EF, Pillai S, Rao P, Robinson WH, Roth KM, Schriefer ME, Slezak T, Snyder J, Steere AC, Witkowski J, Wong SJ, Schutzer SE. Advances in Serodiagnostic Testing for Lyme Disease Are at Hand. Clinical Infectious Diseases 2017, 66: 1133-1139. PMID: 29228208, PMCID: PMC6019075, DOI: 10.1093/cid/cix943.Peer-Reviewed Original ResearchConceptsSerodiagnostic testingEnzyme immunoassayLyme diseaseIndirect fluorescence antibodySerologic assaysImmunoglobulin MEarly infectionFluorescence antibodyTesting protocolAlternative testing protocolsBorrelia burgdorferiB. burgdorferiWestern immunoblotAntigenDiseaseAntibodiesBurgdorferiLow sensitivitySynthetic peptidesAssays
2016
Variable Major Proteins as Targets for Specific Antibodies against Borrelia miyamotoi
Wagemakers A, Koetsveld J, Narasimhan S, Wickel M, Deponte K, Bleijlevens B, Jahfari S, Sprong H, Karan LS, Sarksyan DS, van der Poll T, Bockenstedt LK, Bins AD, Platonov AE, Fikrig E, Hovius JW. Variable Major Proteins as Targets for Specific Antibodies against Borrelia miyamotoi. The Journal Of Immunology 2016, 196: 4185-4195. PMID: 27076681, PMCID: PMC5008243, DOI: 10.4049/jimmunol.1600014.Peer-Reviewed Original ResearchConceptsVariable major proteinsB. miyamotoiSerologic testsC3H/HeN miceBorrelia miyamotoiHumoral immune responseInfected SCID miceTick-borne relapsing feverIgM reactivityHumoral immunityHeN miceIgM productionPassive transferSCID miceSerologic assaysCurrent serologic assaysImmune responseImmune serumRelapsing feverSpecific antibodiesProtein 1MiyamotoiIxodes ticksPatientsFever spirochetes
2014
Borrelia miyamotoi sensu lato Seroreactivity and Seroprevalence in the Northeastern United States - Volume 20, Number 7—July 2014 - Emerging Infectious Diseases journal - CDC
Krause PJ, Narasimhan S, Wormser GP, Barbour AG, Platonov AE, Brancato J, Lepore T, Dardick K, Mamula M, Rollend L, Steeves TK, Diuk-Wasser M, Usmani-Brown S, Williamson P, Sarksyan DS, Fikrig E, Fish D, . Borrelia miyamotoi sensu lato Seroreactivity and Seroprevalence in the Northeastern United States - Volume 20, Number 7—July 2014 - Emerging Infectious Diseases journal - CDC. Emerging Infectious Diseases 2014, 20: 1183-1190. PMID: 24960072, PMCID: PMC4073859, DOI: 10.3201/eid2007.131587.Peer-Reviewed Original ResearchConceptsB. burgdorferiInfectious Diseases journal - CDCSeroprevalence of IgGB. burgdorferi antigensLyme disease endemic areaDisease-endemic areasAntibody testingHealthy personsLyme diseaseSerum samplesSeroprevalenceBurgdorferiInfectionSame tickUnited StatesSensu latoArea residentsPersonsBorrelia spSeroreactivityIgGAntigenDiseaseSerum
2006
γδ T Cells Facilitate Adaptive Immunity against West Nile Virus Infection in Mice
Wang T, Gao Y, Scully E, Davis CT, Anderson JF, Welte T, Ledizet M, Koski R, Madri JA, Barrett A, Yin Z, Craft J, Fikrig E. γδ T Cells Facilitate Adaptive Immunity against West Nile Virus Infection in Mice. The Journal Of Immunology 2006, 177: 1825-1832. PMID: 16849493, DOI: 10.4049/jimmunol.177.3.1825.Peer-Reviewed Original ResearchMeSH KeywordsAdoptive TransferAnimalsCD8-Positive T-LymphocytesGenetic Predisposition to DiseaseImmunity, CellularImmunity, InnateImmunization, SecondaryImmunoglobulin GImmunoglobulin MImmunologic MemoryLymphocyte DepletionMiceMice, Inbred C57BLMice, KnockoutReceptors, Antigen, T-Cell, gamma-deltaRecurrenceT-Lymphocyte SubsetsWest Nile FeverWest Nile virusConceptsGammadelta T cellsWild-type miceT cellsWN virus infectionPrimary infectionVirus infectionWN virusNaive miceSecondary challengeImmune responseAdaptive immunityCD8 memory T cellsWest Nile virus infectionMemory T cellsProtective immune responseAdaptive immune responsesAdoptive transferWest Nile virusAb responsesLethal infectionViral challengeFatal meningoencephalitisSecondary infectionInfectionMice
2005
A recombinant envelope protein vaccine against West Nile virus
Ledizet M, Kar K, Foellmer HG, Wang T, Bushmich SL, Anderson JF, Fikrig E, Koski RA. A recombinant envelope protein vaccine against West Nile virus. Vaccine 2005, 23: 3915-3924. PMID: 15917113, DOI: 10.1016/j.vaccine.2005.03.006.Peer-Reviewed Original Research
2004
Preferential Presence of Decorin-Binding Protein B (BBA25) and BBA50 Antibodies in Cerebrospinal Fluid of Patients with Neurologic Lyme Disease
Fikrig E, Coyle PK, Schutzer SE, Chen M, Deng Z, Flavell RA. Preferential Presence of Decorin-Binding Protein B (BBA25) and BBA50 Antibodies in Cerebrospinal Fluid of Patients with Neurologic Lyme Disease. Journal Of Clinical Microbiology 2004, 42: 1243-1246. PMID: 15004083, PMCID: PMC356844, DOI: 10.1128/jcm.42.3.1243-1246.2004.Peer-Reviewed Original Research
2003
An open-label, nonrandomized, single-center, prospective extension, clinical trial of booster dose schedules to assess the safety profile and immunogenicity of recombinant outer-surface protein A (OspA) Lyme disease vaccine
Schoen RT, Deshefy-Longhi T, Van-Hoecke C, Buscarino C, Fikrig E. An open-label, nonrandomized, single-center, prospective extension, clinical trial of booster dose schedules to assess the safety profile and immunogenicity of recombinant outer-surface protein A (OspA) Lyme disease vaccine. Clinical Therapeutics 2003, 25: 210-224. PMID: 12637121, DOI: 10.1016/s0149-2918(03)90027-0.Peer-Reviewed Original ResearchConceptsLyme disease vaccineFirst booster doseSecond booster doseBooster doseAdverse eventsBooster dosesPrimary seriesMonth 24Disease vaccineClinical trialsEfficacy trialsImmune responseIncidence of AEsPattern of AEsLyme diseaseMost adverse eventsGeometric mean titersTotal IgG antibodiesProportion of subjectsPositive test resultsSeroprotective levelsBooster vaccinationMonth 36Third vaccinationDiary cards
2002
Cyclooxygenase 2 activity modulates the severity of murine Lyme arthritis
Anguita J, Samanta S, Ananthanarayanan SK, Revilla B, Geba GP, Barthold SW, Fikrig E. Cyclooxygenase 2 activity modulates the severity of murine Lyme arthritis. Pathogens And Disease 2002, 34: 187-191. PMID: 12423770, PMCID: PMC4307933, DOI: 10.1111/j.1574-695x.2002.tb00623.x.Peer-Reviewed Original ResearchConceptsJoint inflammationCOX-2B. burgdorferi-specific antibodyBurgdorferi-specific antibodiesOnset of arthritisDegree of inflammationNoninfectious inflammatory diseasesCyclooxygenase-2 activityCOX-2 activityB. burgdorferiCOX-2 gene expressionMurine Lyme arthritisSpecific inhibitionLyme arthritisCytokine responsesInfectious arthritisRheumatoid arthritisInfected miceInflammatory diseasesInducible isoformMurine jointsArthritisInflammationMRNA expressionLyme diseaseComparative reactivity of human sera to recombinant VlsE and other Borrelia burgdorferi antigens in class-specific enzyme-linked immunosorbent assays for Lyme borreliosis
Magnarelli LA, Lawrenz M, Norris SJ, Fikrig E. Comparative reactivity of human sera to recombinant VlsE and other Borrelia burgdorferi antigens in class-specific enzyme-linked immunosorbent assays for Lyme borreliosis. Journal Of Medical Microbiology 2002, 51: 649-655. PMID: 12171295, DOI: 10.1099/0022-1317-51-8-649.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayIgG antibodiesErythema migransIgM antibodiesLyme borreliosisPhysician-diagnosed erythema migransOnset of illnessVlsE antigenB. burgdorferi infectionBorrelia burgdorferi antigensEnzyme-linked immunosorbentHuman granulocytic ehrlichiosisHuman serumOuter surface protein CRheumatoid arthritisBorrelia burgdorferi sensu strictoOral infectionBurgdorferi infectionFalse-positive reactionsBurgdorferi sensu strictoSera crossLouse-borneLaboratory diagnosisNormal serumGranulocytic ehrlichiosisA Recombinant Envelope Protein-Based Enzyme-Linked Immunosorbent Assay for West Nile Virus Serodiagnosis
Wang T, Magnarelli LA, Anderson JF, Gould LH, Bushmich SL, Wong SJ, Fikrig E. A Recombinant Envelope Protein-Based Enzyme-Linked Immunosorbent Assay for West Nile Virus Serodiagnosis. Vector-Borne And Zoonotic Diseases 2002, 2: 105-109. PMID: 12653304, DOI: 10.1089/153036602321131904.Peer-Reviewed Original Research
2001
Salp25D, an Ixodes scapularis Antioxidant, Is 1 of 14 Immunodominant Antigens in Engorged Tick Salivary Glands
Das S, Banerjee G, DePonte K, Marcantonio N, Kantor F, Fikrig E. Salp25D, an Ixodes scapularis Antioxidant, Is 1 of 14 Immunodominant Antigens in Engorged Tick Salivary Glands. The Journal Of Infectious Diseases 2001, 184: 1056-1064. PMID: 11574922, DOI: 10.1086/323351.Peer-Reviewed Original ResearchAmino Acid SequenceAnimalsAntigensAntioxidantsCattleCloning, MolecularConsensus SequenceEnzyme-Linked Immunosorbent AssayFemaleGene LibraryGlutathione PeroxidaseGuinea PigsHumansImmunoglobulin GInsect ProteinsIxodesMiceMice, Inbred C3HMolecular Sequence DataNematodaRabbitsRNA, MessengerSalivary GlandsSequence Homology, Amino AcidSpecies SpecificityTime FactorsReactivity of serum samples of dogs and horses tested by use of class-specific recombinant-based enzyme-linked immunosorbent assays for detection of granulocytic ehrlichiosis.
Magnarelli LA, Ijdo JW, Van Andel AE, Wu C, Oliver JH, Fikrig E. Reactivity of serum samples of dogs and horses tested by use of class-specific recombinant-based enzyme-linked immunosorbent assays for detection of granulocytic ehrlichiosis. American Journal Of Veterinary Research 2001, 62: 1365-9. PMID: 11560261, DOI: 10.2460/ajvr.2001.62.1365.Peer-Reviewed Original ResearchConceptsIgG antibodiesSerum samplesHGE agentGranulocytic ehrlichiosisClient-owned dogsDog serum samplesHuman granulocytic ehrlichiosis (HGE) agentEnzyme-linked immunosorbentPositive resultsWestern blot analysisTick-infested areasEhrlichia phagocytophila genogroupTotal immunoglobulinHorse serum samplesPromyelocytic leukemia cellsClinical relevanceHuman promyelocytic leukemia cellsProtein antigensIgMELISADogsLeukemia cellsAntibodiesBlot analysisAntigenRecombinant Protein-44-Based Class-Specific Enzyme-Linked Immunosorbent Assays for Serologic Diagnosis of Human Granulocytic Ehrlichiosis
Magnarelli L, IJdo J, Wu C, Fikrig E. Recombinant Protein-44-Based Class-Specific Enzyme-Linked Immunosorbent Assays for Serologic Diagnosis of Human Granulocytic Ehrlichiosis. European Journal Of Clinical Microbiology & Infectious Diseases 2001, 20: 482-485. PMID: 11561804, DOI: 10.1007/s100960100542.Peer-Reviewed Original ResearchConceptsPolyvalent enzyme-linked immunosorbent assaysClass-specific enzyme-linked immunosorbent assaysEnzyme-linked immunosorbent assayIndirect fluorescent-antibody analysisFluorescent antibody analysisImmunosorbent assaySpecificity testsIgG antibodiesHigh sensitivityHuman granulocytic ehrlichiosisHuman serumGranulocytic ehrlichiosisWhole cell antigenAntibody analysisImmunoglobulin M antibodiesOnset of illnessSerologic diagnosisSerum samplesHuman granulocytic ehrlichiosis (HGE) agentPatient 3False-positive resultsIgM antibodiesM antibodiesTotal immunoglobulinTotal antibody
2000
Serologic Diagnosis of Lyme Borreliosis by Using Enzyme-Linked Immunosorbent Assays with Recombinant Antigens
Magnarelli L, Ijdo J, Padula S, Flavell R, Fikrig E. Serologic Diagnosis of Lyme Borreliosis by Using Enzyme-Linked Immunosorbent Assays with Recombinant Antigens. Journal Of Clinical Microbiology 2000, 38: 1735-1739. PMID: 10790090, PMCID: PMC86574, DOI: 10.1128/jcm.38.5.1735-1739.2000.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayHuman granulocytic ehrlichiosisRecombinant antigensClass-specific enzyme-linked immunosorbent assaysIgG antibodiesAntibody reactivityLyme borreliosisSerum samplesImmunoblotting of seraImmunoglobulin M antibodiesLow antibody titersB. burgdorferiEnzyme-linked immunosorbentWhole cell antigenAnalysis of serumWestern blot analysisOuter surface protein CErythema migransRheumatoid arthritisAntibody titersIgM antibodiesM antibodiesBorrelia burgdorferi sensu strictoOral infectionSurface protein CGamma Interferon Dominates the Murine Cytokine Response to the Agent of Human Granulocytic Ehrlichiosis and Helps To Control the Degree of Early Rickettsemia
Akkoyunlu M, Fikrig E. Gamma Interferon Dominates the Murine Cytokine Response to the Agent of Human Granulocytic Ehrlichiosis and Helps To Control the Degree of Early Rickettsemia. Infection And Immunity 2000, 68: 1827-1833. PMID: 10722570, PMCID: PMC97354, DOI: 10.1128/iai.68.4.1827-1833.2000.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBacteremiaConcanavalin ACytokinesDisease ProgressionDose-Response Relationship, DrugEhrlichiosisEnzyme-Linked Immunosorbent AssayFemaleHL-60 CellsHumansImmunoglobulin GInterferon-gammaMiceMice, Inbred C3HNeutrophilsReverse Transcriptase Polymerase Chain ReactionRickettsiaSpleenTime FactorsT-LymphocytesConceptsIFN-gamma-deficient miceHuman granulocytic ehrlichiosisIFN-gamma levelsDay 8Cytokine responsesIFN-gammaGamma interferonCells/HGE bacteriaGranulocytic ehrlichiosisIFN-gamma-independent mechanismMurine cytokine responsesTime pointsAgent of HGEMore IFN-gammaC3H/HeNLess interleukin-4IFN-gamma responsesMurine infection modelLater time pointsObligate intracellular bacteriumTh1 phenotypeC57BL/6 miceDNA burdenImmunocompetent mice
1999
The Immunoglobulin (IgG) Antibody Response to OspA and OspB Correlates with Severe and Prolonged Lyme Arthritis and the IgG Response to P35 Correlates with Mild and Brief Arthritis
Akin E, McHugh G, Flavell R, Fikrig E, Steere A. The Immunoglobulin (IgG) Antibody Response to OspA and OspB Correlates with Severe and Prolonged Lyme Arthritis and the IgG Response to P35 Correlates with Mild and Brief Arthritis. Infection And Immunity 1999, 67: 173-181. PMID: 9864212, PMCID: PMC96293, DOI: 10.1128/iai.67.1.173-181.1999.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAntigens, BacterialAntigens, SurfaceArthritis, InfectiousBacterial Outer Membrane ProteinsBacterial ProteinsBacterial VaccinesBorrelia burgdorferi GroupChildChild, PreschoolFemaleHumansImmunoglobulin GLipoproteinsLongitudinal StudiesLyme DiseaseMaleMiddle AgedSeverity of Illness IndexConceptsAntibody responseIgG antibodiesEarly arthritisLyme arthritisIgG responsesEarly infectionDuration of arthritisChronic Lyme arthritisIgG antibody responseSerial serum samplesBorrelia burgdorferi proteinsSubsequent arthritisB. burgdorferi proteinsSurface protein CC-terminal epitopeImmune responseArthritisSubsequent severityNatural historyLyme diseasePatientsSerum samplesImmunoglobulin GProtein CInfection
1998
Human Exposure to a Granulocytic Ehrlichia and Other Tick-Borne Agents in Connecticut
Magnarelli L, Ijdo J, Anderson J, Padula S, Flavell R, Fikrig E. Human Exposure to a Granulocytic Ehrlichia and Other Tick-Borne Agents in Connecticut. Journal Of Clinical Microbiology 1998, 36: 2823-2827. PMID: 9738027, PMCID: PMC105071, DOI: 10.1128/jcm.36.10.2823-2827.1998.Peer-Reviewed Original ResearchConceptsHuman monocytic ehrlichiosisWestern blot analysisTick-borne agentsProbable human casesTick-borne infectionsHuman granulocytic ehrlichiosis (HGE) agentBlot analysisSerologic testingMarked leukopeniaSeropositive specimensPositive seraHGE agentLyme borreliosisBabesia microtiHuman casesMonocytic ehrlichiosisEhrlichia equiBorrelia burgdorferiGranulocytic EhrlichiaEhrlichia chaffeensisFrequent detectionHuman exposurePatientsInfectionAntibodiesHeat shock protein 70 of the agent of human granulocytic ehrlichiosis binds to Borrelia burgdorferi antibodies.
Ijdo J, Zhang Y, Anderson M, Goldberg D, Fikrig E. Heat shock protein 70 of the agent of human granulocytic ehrlichiosis binds to Borrelia burgdorferi antibodies. MSphere 1998, 5: 118-20. PMID: 9455892, PMCID: PMC121403, DOI: 10.1128/cdli.5.1.118-120.1998.Peer-Reviewed Original ResearchConceptsHuman granulocytic ehrlichiosisEhrlichial antigenHSP-70Borrelia burgdorferi antibodiesSerologic test resultsHeat shock protein 70Shock protein 70Burgdorferi antibodiesPatient seraGranulocytic ehrlichiosisLyme diseaseFirst weekHeat shock proteinsLyme disease spirochetePrior exposureImmunoglobulin GProtein 70Immunoblot analysisAntigenShock proteinsSerumAntibodiesPatientsIllnessIgG
1997
The Early Humoral Response in Human Granulocytic Ehrlichiosis
IJdo J, Zhang Y, Hodzic E, Magnarelli L, Wilson M, Telford S, Barthold S, Fikrig E. The Early Humoral Response in Human Granulocytic Ehrlichiosis. The Journal Of Infectious Diseases 1997, 176: 687-692. PMID: 9291316, DOI: 10.1086/514091.Peer-Reviewed Original ResearchConceptsHuman granulocytic ehrlichiosisAntibody responseGranulocytic ehrlichiosisHGE agentC3H/HeJ miceEarly humoral responseEarly antibody responseStrong antibody responseHumoral responseHeJ miceClinical infectionPatientsAntigenHL-60 cellsMiceInfectionIgGEhrlichiosisSerumWeeksResponseIgMAgentsDiseaseDiagnosis