2001
OspA Immunization Decreases Transmission of Borrelia burgdorferi Spirochetes from Infected Peromyscus leucopus Mice to Larval Ixodes scapularis Ticks
Tsao J, Barbour A, Luke C, Fikrig E, Fish D. OspA Immunization Decreases Transmission of Borrelia burgdorferi Spirochetes from Infected Peromyscus leucopus Mice to Larval Ixodes scapularis Ticks. Vector-Borne And Zoonotic Diseases 2001, 1: 65-74. PMID: 12653137, DOI: 10.1089/153036601750137705.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, WildAntibodies, BacterialAntigens, SurfaceArachnid VectorsBacterial Outer Membrane ProteinsBacterial VaccinesBorrelia burgdorferiDisease ReservoirsDose-Response Relationship, ImmunologicEnzyme-Linked Immunosorbent AssayFluorescent Antibody Technique, DirectHost-Parasite InteractionsIxodesLarvaLipoproteinsLyme DiseasePeromyscusConceptsControl miceUninfected larval ticksInfection prevalenceLarval Ixodes scapularis ticksB. burgdorferiNumber of spirochetesEnzyme-linked immunosorbentBorrelia burgdorferi spirochetesLevel of immunizationReservoir hostsConcentration of antibodyBorrelia burgdorferi transmissionIxodes scapularis ticksSecond vaccinationImmunized miceInfected miceLarval ticksPrior infectionVaccination programSubsequent riskDecrease transmissionOspA vaccinationDirect immunofluorescenceImmune responseXenodiagnostic ticks
1999
Influence of Outer Surface Protein A Antibody onBorrelia burgdorferi within Feeding Ticks
de Silva A, Zeidner N, Zhang Y, Dolan M, Piesman J, Fikrig E. Influence of Outer Surface Protein A Antibody onBorrelia burgdorferi within Feeding Ticks. Infection And Immunity 1999, 67: 30-35. PMID: 9864192, PMCID: PMC96273, DOI: 10.1128/iai.67.1.30-35.1999.Peer-Reviewed Original ResearchConceptsAntibody levelsSalivary glandsAntibody titersOspA antibodiesBorrelia burgdorferiLow antibody levelsNumber of spirochetesDensity of spirochetesEnzyme-linked immunosorbentFeeding ticksSurface protein AAbility of spirochetesOuter surface protein AProtective epitopesCompetitive enzyme-linked immunosorbentOuter surface proteinsSpirochetal agentLyme diseaseAntibodiesB. burgdorferiTitersIxodes ticksGlandBurgdorferiSpirochetes
1995
Circumvention of outer surface protein A immunity by host-adapted Borrelia burgdorferi
Barthold SW, Fikrig E, Bockenstedt LK, Persing DH. Circumvention of outer surface protein A immunity by host-adapted Borrelia burgdorferi. Infection And Immunity 1995, 63: 2255-2261. PMID: 7768606, PMCID: PMC173294, DOI: 10.1128/iai.63.6.2255-2261.1995.Peer-Reviewed Original ResearchConceptsInfectious doseInfected skinCultured Borrelia burgdorferiBorrelia burgdorferiLow-dose infectionMedian infectious doseNumber of spirochetesSurface protein ASyringe challengeB. burgdorferi OspAOuter surface protein AChallenge doseDisseminated infectionHost-adapted spirochetesSkin transplantsSyngenic miceOuter surface proteinsMiceHyperimmune serumDoseImmunologic recognitionInfectionSeroconversionTransplantationDisease development
1994
Partial destruction of Borrelia burgdorferi within ticks that engorged on OspE- or OspF-immunized mice
Nguyen TP, Lam TT, Barthold SW, Telford SR, Flavell RA, Fikrig E. Partial destruction of Borrelia burgdorferi within ticks that engorged on OspE- or OspF-immunized mice. Infection And Immunity 1994, 62: 2079-2084. PMID: 8168973, PMCID: PMC186469, DOI: 10.1128/iai.62.5.2079-2084.1994.Peer-Reviewed Original ResearchConceptsEarly-stage disease patientsMajority of patientsLate-stage diseaseSerum of miceLyme disease vaccineB. burgdorferiB. burgdorferi organismsExamination of seraNumber of spirochetesOuter surface protein ESyringe challengeDisease patientsImmune responseDisease vaccinePatientsHigh titersLyme diseaseMiceAntibodiesDiseaseSerumBurgdorferiBorreliaDaysProtein E