2003
Invasion and survival strategies of Anaplasma phagocytophilum
Carlyon JA, Fikrig E. Invasion and survival strategies of Anaplasma phagocytophilum. Cellular Microbiology 2003, 5: 743-754. PMID: 14531890, DOI: 10.1046/j.1462-5822.2003.00323.x.Peer-Reviewed Original ResearchConceptsAnaplasma phagocytophilumA. phagocytophilumHuman granulocytic ehrlichiosisHostile intracellular environmentTick-borne zoonosisObligate intracellular bacteriumHost neutrophilsNeutrophil adhesionInnate immunityPolymorphonuclear leucocytesAetiological agentGranulocytic ehrlichiosisIntracellular bacteriumNeutrophilsMicrobicidal activityPhagocytophilumHost cellsProteolytic compoundsAcidic lysosomesBacteria-containing phagosomesKey playersLeucocytesImmunityEhrlichiosisPhagocytes
2002
Differential Expression of the p44 Gene Family in the Agent of Human Granulocytic Ehrlichiosis
IJdo JW, Wu C, Telford SR, Fikrig E. Differential Expression of the p44 Gene Family in the Agent of Human Granulocytic Ehrlichiosis. Infection And Immunity 2002, 70: 5295-5298. PMID: 12183586, PMCID: PMC128253, DOI: 10.1128/iai.70.9.5295-5298.2002.Peer-Reviewed Original ResearchConceptsP44 geneDifferential expressionHuman granulocytic ehrlichiosisGene familyAntigenic variationHGE bacteriaTransmission feedingGranulocytic ehrlichiosisGenesHypervariable regionInfected C3H miceP44 expressionTick transmissionExpressionSCID miceC3H miceHGE infectionMurine modelInfected ticksSalivary glandsNymphal ticksMiceTicksEhrlichiosisBacteria
2001
Evaluation of a polyvalent enzyme-linked immunosorbent assay incorporating a recombinant p44 antigen for diagnosis of granulocytic ehrlichiosis in dogs and horses.
Magnarelli LA, Ijdo JW, Van Andel AE, Wu C, Fikrig E. Evaluation of a polyvalent enzyme-linked immunosorbent assay incorporating a recombinant p44 antigen for diagnosis of granulocytic ehrlichiosis in dogs and horses. American Journal Of Veterinary Research 2001, 62: 29-32. PMID: 11197555, DOI: 10.2460/ajvr.2001.62.29.Peer-Reviewed Original ResearchConceptsEquine samplesPolyvalent ELISAPolyvalent enzyme-linked immunosorbentGranulocytic ehrlichiosisIFA stainingEquine serum samplesE risticiiEhrlichia canisCanine serum samplesCanine samplesSpecific recombinant antigensE equiHorsesEhrlichiosisPercentage of samplesIndirect fluorescent antibody stainingRecombinant antigensDogsWhole cell antigenCanisEquiELISA resultsSerum samplesPresent studyFluorescent antibody staining
2000
Granulocytic Ehrlichiosis in Mice Deficient in Phagocyte Oxidase or Inducible Nitric Oxide Synthase
Banerjee R, Anguita J, Fikrig E. Granulocytic Ehrlichiosis in Mice Deficient in Phagocyte Oxidase or Inducible Nitric Oxide Synthase. Infection And Immunity 2000, 68: 4361-4362. PMID: 10858261, PMCID: PMC101771, DOI: 10.1128/iai.68.7.4361-4362.2000.Peer-Reviewed Original ResearchThe Emergence of Another Tickborne Infection in the 12-Town Area around Lyme, Connecticut: Human Granulocytic Ehrlichiosis
IJdo J, Meek J, Cartter M, Magnarelli L, Wu C, Tenuta S, Fikrig E, Ryder R. The Emergence of Another Tickborne Infection in the 12-Town Area around Lyme, Connecticut: Human Granulocytic Ehrlichiosis. The Journal Of Infectious Diseases 2000, 181: 1388-1393. PMID: 10751139, DOI: 10.1086/315389.Peer-Reviewed Original ResearchConceptsHuman granulocytic ehrlichiosisTickborne infectionProspective population-based surveillanceGranulocytic ehrlichiosisCommon tickborne infectionPopulation-based surveillancePrimary care providersSubset of seraIndirect fluorescent antibody methodIllness suggestiveCare providersLaboratory evidenceImportant causeProbable casesFluorescent antibody methodImmunoblot assayLyme diseaseInfectionAntibody methodIncidenceEhrlichiosisLymeMorbidityDiseaseSuggestive
1998
Human granulocytic ehrlichiosis, a tick-borne disease.
Ijdo JW, Fikrig E. Human granulocytic ehrlichiosis, a tick-borne disease. Nederlands Tijdschrift Voor Geneeskunde 1998, 142: 1778-81. PMID: 9856144.Peer-Reviewed Original ResearchConceptsPresence of leukopeniaRecent tick biteDay of admissionWhite cell countPeripheral blood smearHuman granulocytic ehrlichiosisElevated transaminasesTick-borne diseaseSevere headachePlatelet countTick biteNormal limitsCell countBlood smearsGranulocytic ehrlichiosisDays durationFull recoveryMyalgiaEhrlichiosisHeadacheWeeksLaboratory resultsLaboratory testsDurationCountCloning of the Gene Encoding the 44-Kilodalton Antigen of the Agent of Human Granulocytic Ehrlichiosis and Characterization of the Humoral Response
Ijdo J, Sun W, Zhang Y, Magnarelli L, Fikrig E. Cloning of the Gene Encoding the 44-Kilodalton Antigen of the Agent of Human Granulocytic Ehrlichiosis and Characterization of the Humoral Response. Infection And Immunity 1998, 66: 3264-3269. PMID: 9632594, PMCID: PMC108341, DOI: 10.1128/iai.66.7.3264-3269.1998.Peer-Reviewed Original ResearchConceptsHuman granulocytic ehrlichiosisGranulocytic ehrlichiosisAgent of HGESera of patientsBorrelia burgdorferi infectionMsp-2 genesElicit specific antibodiesHumoral responseBurgdorferi infectionMurine infectionHealthy individualsPatientsSerumSpecific antibodiesHL-60 cellsInfectionAntibodiesEhrlichia chaffeensisAntigenMiceEhrlichiosisGenomic DNA expression libraryMultigene familyGlutathione transferase fusion proteinFusion proteinGranulocytic Ehrlichiosis in Tick-Immune Guinea Pigs
Das S, Deponte K, Marcantonio N, Ijdo J, Hodzic E, Katavolos P, Barthold S, Telford S, Kantor F, Fikrig E. Granulocytic Ehrlichiosis in Tick-Immune Guinea Pigs. Infection And Immunity 1998, 66: 1803-1805. PMID: 9529119, PMCID: PMC108126, DOI: 10.1128/iai.66.4.1803-1805.1998.Peer-Reviewed Original Research
1997
Immunization against the agent of human granulocytic ehrlichiosis in a murine model.
Sun W, IJdo JW, Telford SR, Hodzic E, Zhang Y, Barthold SW, Fikrig E. Immunization against the agent of human granulocytic ehrlichiosis in a murine model. Journal Of Clinical Investigation 1997, 100: 3014-3018. PMID: 9399947, PMCID: PMC508513, DOI: 10.1172/jci119855.Peer-Reviewed Original ResearchConceptsHuman granulocytic ehrlichiosisGranulocytic ehrlichiosisC3H/HeN miceAgent of HGEMimic human diseaseTick-borne infectionsTransient neutropeniaHeN miceMurine modelPolymorphonuclear leukocytesTick-borne pathogensAoHGEMiceImmunityEhrlichiosisSyringe inoculationHuman diseasesAgentsNeutropeniaImmunizationInfectionLeukocytesDiseaseAntibodiesAntibodies to Ehrlichia equi in dogs from the northeastern United States.
Magnarelli L, IJdo J, Anderson J, Madigan J, Dumler J, Fikrig E. Antibodies to Ehrlichia equi in dogs from the northeastern United States. Journal Of The American Veterinary Medical Association 1997, 211: 1134-7. PMID: 9364226, DOI: 10.2460/javma.1997.211.09.1134.Peer-Reviewed Original ResearchConceptsE equiIndirect fluorescent antibodyWestern blot analysisSerologic testing methodsFluorescent antibodyBlot analysisTick-infested areasSeropositive dogsIll dogsMarked leukopeniaNormal dogsSubclinical infectionSerologic analysisEtiologic agentLaboratory diagnosisEnd pointGranulocytic ehrlichiosisEhrlichia equiDogsAntibodiesHuman isolatesBorrelia burgdorferiDog serumMultiple pathogensEhrlichiosisThe Early Humoral Response in Human Granulocytic Ehrlichiosis
IJdo J, Zhang Y, Hodzic E, Magnarelli L, Wilson M, Telford S, Barthold S, Fikrig E. The Early Humoral Response in Human Granulocytic Ehrlichiosis. The Journal Of Infectious Diseases 1997, 176: 687-692. PMID: 9291316, DOI: 10.1086/514091.Peer-Reviewed Original ResearchConceptsHuman granulocytic ehrlichiosisAntibody responseGranulocytic ehrlichiosisHGE agentC3H/HeJ miceEarly humoral responseEarly antibody responseStrong antibody responseHumoral responseHeJ miceClinical infectionPatientsAntigenHL-60 cellsMiceInfectionIgGEhrlichiosisSerumWeeksResponseIgMAgentsDiseaseDiagnosis