2009
Passage through Ixodes scapularis Ticks Enhances the Virulence of a Weakly Pathogenic Isolate of Borrelia burgdorferi
Adusumilli S, Booth CJ, Anguita J, Fikrig E. Passage through Ixodes scapularis Ticks Enhances the Virulence of a Weakly Pathogenic Isolate of Borrelia burgdorferi. Infection And Immunity 2009, 78: 138-144. PMID: 19822652, PMCID: PMC2798202, DOI: 10.1128/iai.00470-09.Peer-Reviewed Original ResearchConceptsIxodes scapularis ticksCommon tick-borne illnessScapularis ticksTick-borne illnessB. burgdorferi sensu strictoC3H miceBurgdorferi sensu strictoLyme diseaseMiceHigh expression levelsBorrelia burgdorferiB. burgdorferiTick engorgementExpression levelsSpirochetesDiseaseArray analysisSyringe inoculationBurgdorferiPathogenic isolatesIsolatesI. scapularisArthritisVirulencePathogenesis
2002
Differential Expression of the p44 Gene Family in the Agent of Human Granulocytic Ehrlichiosis
IJdo JW, Wu C, Telford SR, Fikrig E. Differential Expression of the p44 Gene Family in the Agent of Human Granulocytic Ehrlichiosis. Infection And Immunity 2002, 70: 5295-5298. PMID: 12183586, PMCID: PMC128253, DOI: 10.1128/iai.70.9.5295-5298.2002.Peer-Reviewed Original ResearchConceptsP44 geneDifferential expressionHuman granulocytic ehrlichiosisGene familyAntigenic variationHGE bacteriaTransmission feedingGranulocytic ehrlichiosisGenesHypervariable regionInfected C3H miceP44 expressionTick transmissionExpressionSCID miceC3H miceHGE infectionMurine modelInfected ticksSalivary glandsNymphal ticksMiceTicksEhrlichiosisBacteria
2000
Borrelia burgdorferi Gene Expression In Vivo and Spirochete Pathogenicity
Anguita J, Samanta S, Revilla B, Suk K, Das S, Barthold S, Fikrig E. Borrelia burgdorferi Gene Expression In Vivo and Spirochete Pathogenicity. Infection And Immunity 2000, 68: 1222-1230. PMID: 10678930, PMCID: PMC97271, DOI: 10.1128/iai.68.3.1222-1230.2000.Peer-Reviewed Original ResearchConceptsC3H/HeN miceBorrelia burgdorferi spirochetesLyme disease pathogenesisNonpathogenic spirochetesSpirochete disseminationHumoral responseImmunocompetent miceHeN miceSCID miceC3H miceImmunodeficient miceImmune serumDisease pathogenesisCarditisArthritisMiceGene expressionGenomic expression libraryDiseaseB. burgdorferiB. burgdorferi N40Borrelia burgdorferi gene expressionMammalian infectionSubsequent developmentInfection
1998
Granulocytic Ehrlichiosis in the Laboratory Mouse
Hodzic E, IJdo J, Feng S, Katavolos P, Sun W, Maretzki C, Fish D, Fikrig E, Telford S, Barthold S. Granulocytic Ehrlichiosis in the Laboratory Mouse. The Journal Of Infectious Diseases 1998, 177: 737-745. PMID: 9498456, DOI: 10.1086/514236.Peer-Reviewed Original ResearchConceptsHuman granulocytic ehrlichiosisMouse passageGranulocytic ehrlichiosisHL-60 cell culturesPeripheral bloodC3H miceLate infectionEarly infectionCell culturesDay 17Day 5Blood smearsNormal valuesDay 24Polymerase chain reaction amplificationHGE agentChain reaction amplificationSpleen smearsMiceInfectionInfectivity assaysBloodLaboratory miceSmearsReaction amplification
1997
Temporal pattern of Borrelia burgdorferi p21 expression in ticks and the mammalian host.
Das S, Barthold SW, Giles SS, Montgomery RR, Telford SR, Fikrig E. Temporal pattern of Borrelia burgdorferi p21 expression in ticks and the mammalian host. Journal Of Clinical Investigation 1997, 99: 987-995. PMID: 9062357, PMCID: PMC507907, DOI: 10.1172/jci119264.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, BacterialAntigens, BacterialAntigens, SurfaceArthritis, InfectiousBacterial Outer Membrane ProteinsBacterial ProteinsBacterial VaccinesBorrelia burgdorferi GroupElectrophoresis, Gel, Pulsed-FieldEnzyme-Linked Immunosorbent AssayFemaleFlagellinFluorescent Antibody Technique, IndirectGene Expression Regulation, BacterialHumansImmunizationImmunization, PassiveImmunoblottingIxodesLipoproteinsLyme DiseaseMiceMice, Inbred C3HPlasmidsPolymerase Chain ReactionRecombinant ProteinsRNA, MessengerTime FactorsConceptsInfected miceHumoral responseLate-stage Lyme diseaseMarkers of infectionCourse of diseaseMurine Lyme borreliosisB. burgdorferiB. burgdorferi infectionHuman humoral responseIxodes dammini ticksBurgdorferi-infected miceLyme arthritisActive immunizationMammalian hostsPassive transferBurgdorferi infectionC3H miceMurine infectionDay 14P21 antibodyP21 expressionLyme borreliosisLyme diseaseMiceInfection
1996
P55, an immunogenic but nonprotective 55-kilodalton Borrelia burgdorferi protein in murine Lyme disease
Feng S, Barthold SW, Telford SR, Fikrig E. P55, an immunogenic but nonprotective 55-kilodalton Borrelia burgdorferi protein in murine Lyme disease. Infection And Immunity 1996, 64: 363-365. PMID: 8557366, PMCID: PMC173770, DOI: 10.1128/iai.64.1.363-365.1996.Peer-Reviewed Original ResearchConceptsProtective responseB. burgdorferi challengeMurine Lyme diseaseStrong antibody responseB. burgdorferi infectionBorrelia burgdorferi proteinsComplete Freund'sControl miceAntibody responseC3H miceBurgdorferi infectionImmunogenic antigensAnti-p55 antibodyLyme diseaseMiceP55B. burgdorferiInfectionAntigenResponseImmunizationFusion proteinTicksDiseaseFreund's
1993
OspA vaccination of mice with established Borrelia burgdorferi infection alters disease but not infection
Fikrig E, Barthold SW, Flavell RA. OspA vaccination of mice with established Borrelia burgdorferi infection alters disease but not infection. Infection And Immunity 1993, 61: 2553-2557. PMID: 8500891, PMCID: PMC280883, DOI: 10.1128/iai.61.6.2553-2557.1993.Peer-Reviewed Original ResearchConceptsBooster injectionComplete Freund's adjuvantVaccination of miceMurine Lyme borreliosisMurine Lyme arthritisFinal booster injectionCommencement of vaccinationNumber of miceSurface protein APostexposure immunizationOuter surface protein APostexposure vaccinationLyme arthritisFreund's adjuvantActive immunizationHeart diseaseC3H miceIncomplete adjuvantVaccinationInfectionLyme borreliosisResolution phaseMiceAdjuvantBorrelia burgdorferiLong-term study of cell-mediated responses to Borrelia burgdorferi in the laboratory mouse
de Souza MS, Smith AL, Beck DS, Terwilliger GA, Fikrig E, Barthold SW. Long-term study of cell-mediated responses to Borrelia burgdorferi in the laboratory mouse. Infection And Immunity 1993, 61: 1814-1822. PMID: 8478071, PMCID: PMC280770, DOI: 10.1128/iai.61.5.1814-1822.1993.Peer-Reviewed Original ResearchConceptsT cell responsesDelayed-type hypersensitivity responseVigorous T cell responseRecipient C3H miceCell-mediated responsesAntigen-specific proliferationBorrelia burgdorferi infectionB cell mitogensAntigen-specific assaysLaboratory miceAdoptive transferImmune dysfunctionHypersensitivity responseResistant miceLymphocyte proliferationLymphoid organsBurgdorferi infectionC3H miceInterleukin-2Prostaglandin productionB cellsDiminished numberLyme borreliosisImpaired proliferationLong-term studies