2017
Mechanoregulated inhibition of formin facilitates contractile actomyosin ring assembly
Zimmermann D, Homa KE, Hocky GM, Pollard LW, De La Cruz EM, Voth GA, Trybus KM, Kovar DR. Mechanoregulated inhibition of formin facilitates contractile actomyosin ring assembly. Nature Communications 2017, 8: 703. PMID: 28951543, PMCID: PMC5614989, DOI: 10.1038/s41467-017-00445-3.Peer-Reviewed Original Research
2012
Actin Network Architecture Can Determine Myosin Motor Activity
Reymann AC, Boujemaa-Paterski R, Martiel JL, Guérin C, Cao W, Chin HF, De La Cruz EM, Théry M, Blanchoin L. Actin Network Architecture Can Determine Myosin Motor Activity. Science 2012, 336: 1310-1314. PMID: 22679097, PMCID: PMC3649007, DOI: 10.1126/science.1221708.Peer-Reviewed Original ResearchPlus‐end directed myosins accelerate actin filament sliding by single‐headed myosin VI
Ramamurthy B, Cao W, De La Cruz EM, Mooseker MS. Plus‐end directed myosins accelerate actin filament sliding by single‐headed myosin VI. Cytoskeleton 2012, 69: 59-69. PMID: 22213699, PMCID: PMC3327287, DOI: 10.1002/cm.21002.Peer-Reviewed Original Research
2011
Actin Filament Dynamics in the Actomyosin VI Complex Is Regulated Allosterically by Calcium–Calmodulin Light Chain
Prochniewicz E, Pierre A, McCullough BR, Chin HF, Cao W, Saunders LP, Thomas DD, De La Cruz EM. Actin Filament Dynamics in the Actomyosin VI Complex Is Regulated Allosterically by Calcium–Calmodulin Light Chain. Journal Of Molecular Biology 2011, 413: 584-592. PMID: 21910998, PMCID: PMC3633491, DOI: 10.1016/j.jmb.2011.08.058.Peer-Reviewed Original ResearchConceptsActin filament dynamicsMyosin VIFilament dynamicsMicrosecond dynamicsCaM-dependent mannerCalmodulin light chainsLight chainActin bindingActin filamentsDependent CaMIQ domainCaM-dependent regulationFluorescence microscopyEnzymatic activityTransient phosphorescence anisotropyATP utilizationFinal anisotropyMicrosecond rotational dynamicsPhosphorescence anisotropyMyosinStructural dynamicsAnisotropy decaySuch modulationActinRegulation
2008
Load-dependent ADP binding to myosins V and VI: Implications for subunit coordination and function
Oguchi Y, Mikhailenko SV, Ohki T, Olivares AO, De La Cruz EM, Ishiwata S. Load-dependent ADP binding to myosins V and VI: Implications for subunit coordination and function. Proceedings Of The National Academy Of Sciences Of The United States Of America 2008, 105: 7714-7719. PMID: 18509050, PMCID: PMC2409399, DOI: 10.1073/pnas.0800564105.Peer-Reviewed Original Research
2005
Thermodynamics of Nucleotide Binding to Actomyosin V and VI: A Positive Heat Capacity Change Accompanies Strong ADP Binding †
Robblee JP, Cao W, Henn A, Hannemann DE, De La Cruz EM. Thermodynamics of Nucleotide Binding to Actomyosin V and VI: A Positive Heat Capacity Change Accompanies Strong ADP Binding †. Biochemistry 2005, 44: 10238-10249. PMID: 16042401, DOI: 10.1021/bi050232g.Peer-Reviewed Original Research
2004
Mechanism of Nucleotide Binding to Actomyosin VI EVIDENCE FOR ALLOSTERIC HEAD-HEAD COMMUNICATION*
Robblee JP, Olivares AO, De La Cruz EM. Mechanism of Nucleotide Binding to Actomyosin VI EVIDENCE FOR ALLOSTERIC HEAD-HEAD COMMUNICATION*. Journal Of Biological Chemistry 2004, 279: 38608-38617. PMID: 15247304, DOI: 10.1074/jbc.m403504200.Peer-Reviewed Original ResearchActinsActomyosinAdenosine DiphosphateAdenosine TriphosphatasesAdenosine TriphosphateAllosteric SiteAnimalsCell LineDose-Response Relationship, DrugHydrogen-Ion ConcentrationInsectaKineticsModels, BiologicalModels, ChemicalModels, StatisticalMuscle, SkeletalMyosin Heavy ChainsNucleotidesProtein BindingPyrenesRabbitsTime FactorsRelating biochemistry and function in the myosin superfamily
De La Cruz EM, Ostap EM. Relating biochemistry and function in the myosin superfamily. Current Opinion In Cell Biology 2004, 16: 61-67. PMID: 15037306, DOI: 10.1016/j.ceb.2003.11.011.Peer-Reviewed Original Research
2002
Actin-induced Closure of the Actin-binding Cleft of Smooth Muscle Myosin*
Yengo CM, De La Cruz EM, Chrin LR, Gaffney DP, Berger CL. Actin-induced Closure of the Actin-binding Cleft of Smooth Muscle Myosin*. Journal Of Biological Chemistry 2002, 277: 24114-24119. PMID: 11959853, DOI: 10.1074/jbc.m111253200.Peer-Reviewed Original Research
2001
Kinetic Mechanism and Regulation of Myosin VI*
De La Cruz E, Ostap E, Sweeney H. Kinetic Mechanism and Regulation of Myosin VI*. Journal Of Biological Chemistry 2001, 276: 32373-32381. PMID: 11423557, DOI: 10.1074/jbc.m104136200.Peer-Reviewed Original ResearchConceptsHeavy chain phosphorylationMyosin VIPhysiological nucleotide concentrationsADP releaseHigh duty ratio motorMolecular basisUnique adaptationsActin filamentsATP bindsATPase cycleNative dimerRate-limiting stepDetailed kinetic analysisChain phosphorylationRegulationNucleotide concentrationsDiffusional encounterMyosinLow affinityMutantsProcessivityKinetic analysisPhosphorylationActinBinds